LOCUS       Exported                5466 bp ds-DNA     circular SYN 05-FEB-2021
DEFINITION  p1: CJ710-CJ711 NL564 1055 bp
            
            p2: CJ712-CJ012 2204 bp pCJ100
            
            p3: CJ015-CJ713 2277 bp pCJ100
            
            cPCR primers
            CJ343-CJ714 779 bp.
ACCESSION   .
VERSION     .
KEYWORDS    pCJ111
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Brian Pfleger Group
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 11, 2021 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          845..1538
                     /organism="Synechococcus sp. PCC 7002"
                     /strain="PCC 7002; ATCC 27264"
                     /mol_type="genomic DNA"
                     /db_xref="ATCC:27264"
                     /db_xref="taxon:32049"
     source          1539..2567
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3825..4324
                     /organism="Synechococcus sp. PCC 7002"
                     /strain="PCC 7002; ATCC 27264"
                     /mol_type="genomic DNA"
                     /db_xref="ATCC:27264"
                     /db_xref="taxon:32049"
     rep_origin      74..662
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    845..1538
                     /note="upstream rbcL transl. fusion"
     misc_feature    845..1344
                     /note="rbcLXS up"
     CDS             845..1030
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="SYNPCC7002_RS09015"
                     /old_locus_tag="SYNPCC7002_A1799"
                     /product="hypothetical protein"
                     /note="SYNPCC7002_RS09015"
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:HMM"
                     /protein_id="WP_083764459.1"
                     /translation="AGSPPTETPNTEVMPTVPESKESLESGEKTPVVGQVYINQLLMTL
                     FPHQNSLNTPNQPDEP"
     gene            845..1030
                     /locus_tag="SYNPCC7002_RS09015"
                     /old_locus_tag="SYNPCC7002_A1799"
                     /note="SYNPCC7002_RS09015"
     misc_feature    1031..3824
                     /note="prbcLCRE-kan cassette"
     misc_feature    1252..1538
                     /note="rbcLXS comp"
     misc_feature    1430
                     /note="+1"
     misc_feature    1464..1538
                     /note="rbcLXS transcriptional fusion cassette"
     misc_feature    1464..1502
                     /note="5'-UTR"
                     /note="arbitrarily assigned"
     CDS             1503..1538
                     /codon_start=1
                     /transl_table=11
                     /gene="rbcL"
                     /locus_tag="SYNPCC7002_RS09010"
                     /old_locus_tag="SYNPCC7002_A1798"
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /note="rbcL"
                     /note="type III RuBisCO; involved in carbon fixation; 
                     Derived by automated computational analysis using gene 
                     prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_012307409.1"
                     /translation="MVQTKSAGFNAG"
     CDS             1539..2567
                     /codon_start=1
                     /note="CRE"
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    2568..2729
                     /note="clac94_10XHisYFP"
     terminator      2651..2698
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    2730..3824
                     /note="kan casette"
     misc_feature    2730..3824
                     /note="kan casette"
     CDS             2844..3659
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      3710..3737
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     terminator      3753..3824
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    3825..4324
                     /note="rbcLXS down"
     CDS             3986..4324
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="SYNPCC7002_RS08995"
                     /old_locus_tag="SYNPCC7002_A1795"
                     /product="DUF2059 domain-containing protein"
                     /note="SYNPCC7002_RS08995"
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_012307406.1"
                     /translation="MNLKFLKSLWATAAIAFAISVNPSLVFAETEPPSETKTALINELR
                     TLTFRDENATQILDLMLQQIQAQSTTMGDGFLGEETDPETLAAIQESVTRITDRIYTLM
                     QDRIDFVAL"
     gene            3986..4324
                     /locus_tag="SYNPCC7002_RS08995"
                     /old_locus_tag="SYNPCC7002_A1795"
                     /note="SYNPCC7002_RS08995"
     misc_feature    4325..844
                     /note="pUC19 backbone"
     promoter        4404..4508
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4509..5369
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
       61 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      181 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      721 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      781 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      841 aagagcagga tcccctccaa ctgaaacacc aaacaccgaa gtgatgccga cggttccaga
      901 atcaaaggaa tccctagaat cgggcgaaaa aacaccagtt gtcgggcaag tttatatcaa
      961 tcaactatta atgaccctat tccctcacca aaattctctc aataccccaa atcaaccaga
     1021 tgagccttag ccccacaaaa ctttcatgat tgcttgagtg aaaattaaat gtttaaagtt
     1081 cttaaaggag attgttgaga aacataaata cttaattcat ctcatttgaa cgctttccct
     1141 ctctaaagat cccgacagaa aacggtttta gcccaatgtc tcattaggta gcatggctga
     1201 attcgagcgg gattttatgg cttttttagg tatttttgta agggtaaaat aggcccatca
     1261 aacagcatta gaaatgctaa tcagcccaaa aaacaaaagc aatctttttt tgttgctaaa
     1321 agataaaaat aagtcgaggc tgtggtaaca tatcccacag attaaagaaa gtcataagac
     1381 ttgaatcttc agaattttaa aaagcagttt tgccaacgta agatttttga agttttcgac
     1441 caacaatacc gttactggta tttgtctgtt aaagataagc atttttgctg gaggaaaacc
     1501 gcatggttca gaccaaatct gctgggttta atgccggttc caacctgctg acggtgcacc
     1561 agaatctgcc ggcactgccg gtcgatgcaa ccagtgacga agtgcgcaaa aatctgatgg
     1621 atatgtttcg tgaccgccaa gccttcagcg aacatacgtg gaaaatgctg ctgtcggttt
     1681 gccgtagctg ggcggcctgg tgtaaactga acaatcgcaa atggtttccg gcagaaccgg
     1741 aagatgtgcg tgactatctg ctgtacctgc aggcacgtgg tctggcagtt aaaaccatcc
     1801 agcaacatct gggccaactg aacatgctgc accgtcgctc tggtctgccg cgtccgagtg
     1861 attccaatgc cgtcagtctg gtgatgcgtc gcattcgtaa agaaaacgtg gatgcaggcg
     1921 aacgcgctaa acaggcactg gcttttgaac gtaccgattt cgaccaagtt cgctctctga
     1981 tggaaaacag tgatcgttgc caggacatcc gcaatctggc attcctgggt attgcttata
     2041 acaccctgct gcgcattgca gaaatcgctc gtattcgcgt gaaagatatc agccgtacgg
     2101 acggcggtcg catgctgatt cacatcggcc gtaccaaaac gctggtttcc accgcgggcg
     2161 tcgaaaaagc cctgtcactg ggtgtcacga aactggtgga acgctggatt tcagtttcgg
     2221 gcgtcgcaga tgacccgaac aattacctgt tttgtcgtgt gcgcaaaaat ggtgttgcag
     2281 ctccgagcgc tacctctcag ctgagtacgc gtgcgctgga aggcatcttc gaagccaccc
     2341 atcgcctgat ttatggcgcg aaagatgaca gcggtcagcg ttacctggca tggtccggtc
     2401 actcagctcg tgttggtgca gcacgtgata tggcacgtgc aggtgtctct atcccggaaa
     2461 ttatgcaggc cggcggttgg acgaacgtga atattgttat gaactatatt cgtaacctgg
     2521 actctgaaac gggtgcgatg gtgcgtctgc tggaagatgg cgactgacga gggcggtgct
     2581 ttggcaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     2641 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgacgg gttttttgtc
     2701 tagatcaacg gcctcaacct actactgggc aacaaagcca cgttgtgtct caaaatctct
     2761 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     2821 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctccaggc
     2881 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     2941 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt
     3001 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     3061 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     3121 atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat
     3181 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     3241 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     3301 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     3361 ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag
     3421 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     3481 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     3541 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     3601 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     3661 cagaattggt taattggttg taacactggc agagcctcta gtatataaac gcagaaaggc
     3721 ccacccgaag gtgagccagt gtgactctag tagagagcgt tcaccgacaa acaacagata
     3781 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgggtttt gttggttttt
     3841 gtgacctgaa tttaatttga atcatgcgga atgcgatcgc cttaggacgg tcgcattttt
     3901 tgtttacgtc taaaattagt cgaaatcccc catcaacgcc atggtagtga ttttgtctta
     3961 acgttattca cccatcaatt tcaaaatgaa cctgaagttt cttaaatctc tctgggctac
     4021 ggcggcgatc gcctttgcca ttagcgtaaa tccgagcctt gtctttgctg aaacggaacc
     4081 cccaagcgaa accaaaactg ccctgatcaa tgaactccgc accttaactt tccgggatga
     4141 aaacgcgacc cagattttgg acttgatgct gcaacagatc caagctcaat ccaccaccat
     4201 gggcgatggc tttttgggtg aagaaacaga tcccgaaacc ctggccgcta tccaagaaag
     4261 tgtcacccgc atcaccgacc gcatttatac cctgatgcaa gacagaattg atttcgttgc
     4321 cctcgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4381 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4501 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4561 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4621 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4681 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4741 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4801 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4861 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4921 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4981 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5041 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5101 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5161 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5221 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5281 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5341 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5401 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5461 atctca
//
