LOCUS       Exported                5345 bp ds-DNA     circular SYN 19-MAY-2020
DEFINITION  p1: CJ975-CJ976 1723 bp pCJ111
            
            p2: CJ977-CJ012 1608 bp pCJ130
            
            p3: CJ015-CJ978 2084 bp pCJ130
            
            
            cPCRs-----------------------------------
            CJ823-CJ342 713 bp.
ACCESSION   .
VERSION     .
KEYWORDS    pCJ155
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Brian Pfleger Group
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 11, 2021 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          859..1358
                     /organism="Synechococcus sp. PCC 7002"
                     /strain="PCC 7002; ATCC 27264"
                     /mol_type="genomic DNA"
                     /db_xref="ATCC:27264"
                     /db_xref="taxon:32049"
     source          1359..1866
                     /organism="Synechococcus sp. PCC 7002"
                     /strain="PCC 7002; ATCC 27264"
                     /mol_type="genomic DNA"
                     /db_xref="ATCC:27264"
                     /db_xref="taxon:32049"
     source          1867..2895
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3718..4217
                     /organism="Synechococcus sp. PCC 7002"
                     /strain="PCC 7002; ATCC 27264"
                     /mol_type="genomic DNA"
                     /db_xref="ATCC:27264"
                     /db_xref="taxon:32049"
     misc_feature    1..858
                     /note="pUC19 backbone"
     rep_origin      88..676
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    859..1358
                     /note="psbEFLJ up"
     CDS             859..1255
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="SYNPCC7002_RS01140"
                     /old_locus_tag="SYNPCC7002_A0229"
                     /product="photosynthesis system II assembly factor Ycf48
                     
                     SYNPCC7002_RS01140"
                     /note="photosynthesis system II assembly factor Ycf48"
                     /note="similar to Arabidopsis thaliana photosystem II 
                     assembly protein; Derived by automated computational 
                     analysis using gene prediction method: Protein Homology."
                     /db_xref="GI:501262847"
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_012305865.1"
                     /translation="PHNRNSSRRLQSMGFNGEDKLWLLARGGVVQFSDDTNPDNAEAWS
                     EPVTPQYRNSVGLLHIGYRTPAELWAVGGSGSVVVSKDGGDTWFRDAALEEIPTNFYRV
                     VFLNENKGFILGQQGVILRYDTSTEAA"
     gene            859..1255
                     /locus_tag="SYNPCC7002_RS01140"
                     /old_locus_tag="SYNPCC7002_A0229"
                     /note="SYNPCC7002_RS01140"
     misc_feature    1359..3717
                     /note="prbcL-CRE-zeo cassette"
     misc_feature    1359..3057
                     /note="prbcL-CRE casette"
     misc_feature    1359..3057
                     /note="prbcLCRE-kan cassette"
     misc_feature    1359..1866
                     /note="upstream rbcL transl. fusion"
     misc_feature    1359..1672
                     /note="rbcLXS up"
     misc_feature    1580..1866
                     /note="rbcLXS comp"
     misc_feature    1792..1866
                     /note="rbcLXS transcriptional fusion cassette"
     misc_feature    1792..1830
                     /note="5'-UTR"
                     /note="arbitrarily assigned"
     CDS             1831..1866
                     /codon_start=1
                     /transl_table=11
                     /gene="rbcL"
                     /locus_tag="SYNPCC7002_RS09010"
                     /old_locus_tag="SYNPCC7002_A1798"
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /note="rbcL"
                     /note="type III RuBisCO; involved in carbon fixation; 
                     Derived by automated computational analysis using gene 
                     prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_012307409.1"
                     /translation="MVQTKSAGFNAG"
     gene            1831..1866
                     /gene="rbcL"
                     /locus_tag="SYNPCC7002_RS09010"
                     /old_locus_tag="SYNPCC7002_A1798"
                     /note="rbcL"
     CDS             1867..2895
                     /codon_start=1
                     /note="CRE"
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    2896..3057
                     /note="YFP kan fusion"
     misc_feature    2896..3057
                     /note="clac94_10XHisYFP"
     terminator      2979..3026
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    3058..3717
                     /note="zeo"
     promoter        3058..3105
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             3124..3495
                     /codon_start=1
                     /note="bleoR"
                     /translation="MKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV
                     TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE
                     FALRDPAGNCVHFVAEEQD"
     misc_feature    3718..4217
                     /note="psbEFLJ down"
     CDS             3845..4217
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="SYNPCC7002_RS01155"
                     /old_locus_tag="SYNPCC7002_A0234"
                     /product="alpha/beta fold hydrolase"
                     /note="SYNPCC7002_RS01155"
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Protein Homology."
                     /db_xref="GI:501262852"
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_012305870.1"
                     /translation="MTMKRQLRDLKVWVMVGLLSLGGAWGQRAIAAETLRLKLGPLQQT
                     LQLEDLETFAETGQVPRNLRPYGVFLDGNLQKFLQRRLQIEPEMADQFFDQLWRSPTGK
                     QILAQIQTALPGTSIQDLQA"
     gene            3845..4217
                     /locus_tag="SYNPCC7002_RS01155"
                     /old_locus_tag="SYNPCC7002_A0234"
                     /note="SYNPCC7002_RS01155"
     misc_feature    4218..5345
                     /note="pUC19 backbone"
     promoter        4297..4401
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4402..5262
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
       61 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      241 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      721 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      781 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      841 gagcgaggaa gcggaagagc cccataaccg gaattcttcc cgtcgcctcc aaagcatggg
      901 ctttaatggc gaagataagc tctggttgtt agcccgtggg ggagttgtgc aatttagcga
      961 cgatacaaat ccagacaatg cagaagcctg gagcgaacca gtgacaccgc agtaccgcaa
     1021 cagtgttggt ttgttgcaca ttggctatcg tacccccgca gaactatggg ccgtcggtgg
     1081 tagtggcagt gttgttgtga gtaaagatgg tggcgacact tggttccggg acgctgccct
     1141 agaagagatc ccgacaaact tttaccgagt cgttttctta aatgagaata aaggcttcat
     1201 ccttgggcaa cagggggtga ttttacggta cgatacatcc acggaagcgg cctaagggca
     1261 acccattcta ttgtgggagt gtctgttaat tccgtatgat ataggctaat ttgtttaata
     1321 agtttttttt cattaacaat tgagaggaga atagtcgccc ccacaaaact ttcatgattg
     1381 cttgagtgaa aattaaatgt ttaaagttct taaaggagat tgttgagaaa cataaatact
     1441 taattcatct catttgaacg ctttccctct ctaaagatcc cgacagaaaa cggttttagc
     1501 ccaatgtctc attaggtagc atggctgaat tcgagcggga ttttatggct tttttaggta
     1561 tttttgtaag ggtaaaatag gcccatcaaa cagcattaga aatgctaatc agcccaaaaa
     1621 acaaaagcaa tctttttttg ttgctaaaag ataaaaataa gtcgaggctg tggtaacata
     1681 tcccacagat taaagaaagt cataagactt gaatcttcag aattttaaaa agcagttttg
     1741 ccaacgtaag atttttgaag ttttcgacca acaataccgt tactggtatt tgtctgttaa
     1801 agataagcat ttttgctgga ggaaaaccgc atggttcaga ccaaatctgc tgggtttaat
     1861 gccggttcca acctgctgac ggtgcaccag aatctgccgg cactgccggt cgatgcaacc
     1921 agtgacgaag tgcgcaaaaa tctgatggat atgtttcgtg accgccaagc cttcagcgaa
     1981 catacgtgga aaatgctgct gtcggtttgc cgtagctggg cggcctggtg taaactgaac
     2041 aatcgcaaat ggtttccggc agaaccggaa gatgtgcgtg actatctgct gtacctgcag
     2101 gcacgtggtc tggcagttaa aaccatccag caacatctgg gccaactgaa catgctgcac
     2161 cgtcgctctg gtctgccgcg tccgagtgat tccaatgccg tcagtctggt gatgcgtcgc
     2221 attcgtaaag aaaacgtgga tgcaggcgaa cgcgctaaac aggcactggc ttttgaacgt
     2281 accgatttcg accaagttcg ctctctgatg gaaaacagtg atcgttgcca ggacatccgc
     2341 aatctggcat tcctgggtat tgcttataac accctgctgc gcattgcaga aatcgctcgt
     2401 attcgcgtga aagatatcag ccgtacggac ggcggtcgca tgctgattca catcggccgt
     2461 accaaaacgc tggtttccac cgcgggcgtc gaaaaagccc tgtcactggg tgtcacgaaa
     2521 ctggtggaac gctggatttc agtttcgggc gtcgcagatg acccgaacaa ttacctgttt
     2581 tgtcgtgtgc gcaaaaatgg tgttgcagct ccgagcgcta cctctcagct gagtacgcgt
     2641 gcgctggaag gcatcttcga agccacccat cgcctgattt atggcgcgaa agatgacagc
     2701 ggtcagcgtt acctggcatg gtccggtcac tcagctcgtg ttggtgcagc acgtgatatg
     2761 gcacgtgcag gtgtctctat cccggaaatt atgcaggccg gcggttggac gaacgtgaat
     2821 attgttatga actatattcg taacctggac tctgaaacgg gtgcgatggt gcgtctgctg
     2881 gaagatggcg actgacgagg gcggtgcttt ggcaggatcc ggctgctaac aaagcccgaa
     2941 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     3001 ctaaacgggt cttgacgggt tttttgtcta gatcaacggc ctcaacctac tactggggtt
     3061 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     3121 accatgaagc tgaccagcgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     3181 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     3241 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     3301 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     3361 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     3421 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     3481 gaggagcagg actaaccgac gtcgacccac tctagaggat ccccgctccg tgtaaatgga
     3541 ggcgctcgtt gatctgagcc ttgccccctg acgaacggcg gtggatggaa gatactgctc
     3601 tcaagtgctg aagcggtagc ttagctcccc gtttcgtgct gatcagtctt tttcaacacg
     3661 taaaaagcgg aggagttttg caattttgtt ggttgtaacg atcctccgtt gcaattgatg
     3721 cacaattcgc tctaattaat tgttgagaca ccacctgctc taattgaggg gtggttttgt
     3781 tttaggaact tctggggcaa tagggagtcc aaaaaagttt ggggataatc gtcaacggtg
     3841 cagtatgact atgaaaagac agttgcggga tctgaaagta tgggtgatgg tgggtctgtt
     3901 gagtttaggt ggagcctggg gtcaaagggc gatcgccgct gaaaccctcc gactaaaatt
     3961 agggccactt caacaaactt tacaattaga agatttagaa acctttgccg aaacgggtca
     4021 agtcccccgc aacctccgac cctatggggt atttttggat ggtaatttgc agaaatttct
     4081 ccaacgacgc ttgcagatcg agccagagat ggcggatcag ttttttgatc agttgtggcg
     4141 atcgcctacg ggaaaacaga ttttggcgca aattcaaacg gccctaccgg gaacttctat
     4201 tcaggatctc caggcgagat acgcctattt ttataggtta atgtcatgat aataatggtt
     4261 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4321 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4381 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4441 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4501 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4561 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4621 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4681 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4741 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4801 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4861 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4921 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4981 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5041 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5101 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5161 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5221 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5281 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5341 atcct
//
