LOCUS       Exported                5948 bp ds-DNA     circular SYN 28-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCJ-ZD1111
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 11, 2021 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1705..2733
                     /label=source
     rep_origin      74..662
                     /label=ori
                     /note="ori"
     misc_feature    845..1704
                     /label=US 6803 rbcL trans. fusion
                     /note="US 6803 rbcL trans. fusion"
     CDS             1669..2733
                     /codon_start=1
                     /note="rbcL-CRE"
                     /translation="MVQAKAGFKAGVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDR
                     QAFSEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLG
                     QLNMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENS
                     DRCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK
                     ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHR
                     LIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLD
                     SETGAMVRLLEDGD"
     misc_feature    1669..1704
                     /label=6803 rbcL
                     /note="6803 rbcL"
     misc_feature    1705..3990
                     /label=prbcLCRE-kan cassette
                     /note="prbcLCRE-kan cassette"
     CDS             1705..2733
                     /label=CRE
                     /note="CRE"
     misc_feature    2734..3990
                     /label=YFP kan fusion
                     /note="YFP kan fusion"
     misc_feature    2734..2895
                     /label=clac94_10XHisYFP
                     /note="clac94_10XHisYFP"
     terminator      2817..2864
                     /label=T7 terminator
                     /note="T7 terminator"
     misc_feature    2896..3990
                     /label=kan casette
                     /note="kan casette"
     CDS             3010..3825
                     /label=KanR
                     /note="KanR"
     terminator      3876..3903
                     /label=T7Te terminator
                     /note="T7Te terminator"
     terminator      3919..3990
                     /label=rrnB T1 terminator
                     /note="rrnB T1 terminator"
     misc_feature    3991..4806
                     /label=DS 6803 rbcLXS
                     /note="DS 6803 rbcLXS"
     misc_feature    4807..844
                     /label=pUC19 backbone
                     /note="pUC19 backbone"
     promoter        4886..4990
                     /label=AmpR promoter
                     /note="AmpR promoter"
     CDS             4991..5059
                     /label=AmpR
                     /note="AmpR"
     CDS             5060..5851
                     /label=AmpR
                     /note="AmpR"
ORIGIN
        1 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
       61 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      181 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      721 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      781 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      841 aagaggccga cggttatatc ttggatttgc gtaacaaccc cggtggctta ctccaggctg
      901 gtattgacat tgctcggttg tggttaccgg aaagcaccat tgtctacacc gttaatcgcc
      961 aaggcaccca ggaaagtttc actgccaatg gagaagcggc gaccgatcgc ccgttggtgg
     1021 tgttggtcaa ccagggtact gccagtgcca gcgaaatttt agccggagct ttgcaggata
     1081 atcagcgggc cactctagtg ggggaaaaaa cctttggtaa gggtttgatt caatccttgt
     1141 ttgaactatc cgatggggcc ggcattgccg tcacggtggc caaatacgaa accccccaac
     1201 atcacgacat ccataaactg ggcattatgc ccgatgaagt ggtggagcaa cccctgatta
     1261 gctttgcgga aattacttcc cccgccgatg tgcaatacca agccgcctta gatttgctca
     1321 ccggaggagt ggcaatcgcc cataaatctt cttcaattcc cgccatggca acggctcaca
     1381 agcccaacta atcaccattt ggacaaaaca tcaggaattc taattagaaa gtccaaaaat
     1441 tgtaatttaa aaaacagtca atggagagca ttgccataag taaaggcatc ccctgcgtga
     1501 taagattacc ttcagaaaac agatagttgc tgggttatcg cagatttttc tcgcaaccaa
     1561 ataactgtaa ataataactg tctctggggc gacggtaggc tttatattgc caaatttcgc
     1621 ccgtgggaga aagctaggct attcaatgtt tatggaggac tgacctagat ggtacaagcc
     1681 aaagcagggt ttaaggcggg cgtatccaac ctgctgacgg tgcaccagaa tctgccggca
     1741 ctgccggtcg atgcaaccag tgacgaagtg cgcaaaaatc tgatggatat gtttcgtgac
     1801 cgccaagcct tcagcgaaca tacgtggaaa atgctgctgt cggtttgccg tagctgggcg
     1861 gcctggtgta aactgaacaa tcgcaaatgg tttccggcag aaccggaaga tgtgcgtgac
     1921 tatctgctgt acctgcaggc acgtggtctg gcagttaaaa ccatccagca acatctgggc
     1981 caactgaaca tgctgcaccg tcgctctggt ctgccgcgtc cgagtgattc caatgccgtc
     2041 agtctggtga tgcgtcgcat tcgtaaagaa aacgtggatg caggcgaacg cgctaaacag
     2101 gcactggctt ttgaacgtac cgatttcgac caagttcgct ctctgatgga aaacagtgat
     2161 cgttgccagg acatccgcaa tctggcattc ctgggtattg cttataacac cctgctgcgc
     2221 attgcagaaa tcgctcgtat tcgcgtgaaa gatatcagcc gtacggacgg cggtcgcatg
     2281 ctgattcaca tcggccgtac caaaacgctg gtttccaccg cgggcgtcga aaaagccctg
     2341 tcactgggtg tcacgaaact ggtggaacgc tggatttcag tttcgggcgt cgcagatgac
     2401 ccgaacaatt acctgttttg tcgtgtgcgc aaaaatggtg ttgcagctcc gagcgctacc
     2461 tctcagctga gtacgcgtgc gctggaaggc atcttcgaag ccacccatcg cctgatttat
     2521 ggcgcgaaag atgacagcgg tcagcgttac ctggcatggt ccggtcactc agctcgtgtt
     2581 ggtgcagcac gtgatatggc acgtgcaggt gtctctatcc cggaaattat gcaggccggc
     2641 ggttggacga acgtgaatat tgttatgaac tatattcgta acctggactc tgaaacgggt
     2701 gcgatggtgc gtctgctgga agatggcgac tgacgagggc ggtgctttgg caggatccgg
     2761 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
     2821 cataacccct tggggcctct aaacgggtct tgacgggttt tttgtctaga tcaacggcct
     2881 caacctacta ctgggcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca
     2941 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     3001 aggggtgtta tgagccatat tcaacgggaa acgtcttgct ccaggccgcg attaaattcc
     3061 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     3121 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     3181 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     3241 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     3301 accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt
     3361 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     3421 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     3481 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     3541 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt
     3601 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     3661 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     3721 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     3781 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3841 tggttgtaac actggcagag cctctagtat ataaacgcag aaaggcccac ccgaaggtga
     3901 gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3961 gtctttcgac tgagcctttc gttttatttg gttacagttt tggcaattac taaaaaactg
     4021 acttcaattc aatgttagcc cgctcccgcg ggttttttgt tgctttttca cagtgactat
     4081 aggtaatcag caacacaata cggccctgtt ctttggacag tttttgtata atgttgaccg
     4141 catcctgacc ggatttttta tctaagtggg gaattgtcaa ttgtcaatta aagctaagtt
     4201 ctactaatgt tttagaaggc attgtcgatt gaaaataagg gttgaatgga gaaaattttg
     4261 agcctttgtc aaagataaaa atttatttca acagtttttt aactagccga accagagaat
     4321 gacccagtgg cgctgacttt gctcccgagt ttttgttaga aattaccctc aagaagtaat
     4381 ctaataataa acctaaccga ataatttccc aggggagtat tccggaaaac catggttaaa
     4441 cttacttgcc atcccccatg gtaaaattgc aacgattttg atcaaagtcc taattttttt
     4501 gtaaagcttt tagtaatcct tctgattttc ccatgaagct catcgattcc cgtggcagaa
     4561 ttttcggcat agtcagcctg ttggatctgg gggccgcctt gatcatcctc atggtagctg
     4621 tgggaatttt cgttctgccg ggcagttctg gcaaaagcat tcttgcccaa gccaacgccg
     4681 cttccattga attgacgacc attgtccggg gattaaacgt attagatccc caggtggtgc
     4741 tggatgagtt taaagccgaa aaaaccaaca tcattattcg caatcaaccg gctggccagg
     4801 tggagggata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4861 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4921 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4981 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5041 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5101 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5161 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5221 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5281 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5341 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5401 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5461 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5521 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5581 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5641 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5701 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5761 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5821 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5881 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5941 taatctca
//
