LOCUS       Exported                9606 bp DNA     circular SYN 22-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Gerace Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3342..3465
                     /label=NEB_Koz_Myc
     primer_bind     3342..3401
                     /label=NEB_Koz_Myc_Bait_F
     primer_bind     3382..3465
                     /label=Koz_Myc_2xGS_F
     regulatory      3382..3390
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3391..3420
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      3391
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    3421..3465
                     /label=3xGSlinker
                     /note="3xGSlinker"
     primer_bind     3421..3437
                     /label=Myc_Bait_F
     primer_bind     complement(3446..3465)
                     /label=V5_TbID__3XGS_R
     primer_bind     3451..3467
                     /label=Myc_Bait_F
     misc_feature    3466..4263
                     /label=EMD_ORF_MM
     primer_bind     complement(4240..4274)
                     /label=Fragment.REV
     primer_bind     4253..4290
                     /label=Vector.FOR
     primer_bind     4264..4283
                     /label=V5_TbID__3XGS_F
     primer_bind     complement(4269..4291)
                     /label=Myc_Bait_R
     primer_bind     complement(4270..4331)
                     /label=NEB_Koz_Myc_Bait_R
     misc_feature    4290..4291
                     /label=IRES
                     /note="/vntifkey=21"
     primer_bind     complement(4544..4561)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4771..4790
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4952..5551
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4952..4971)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5448..5468
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5575..6163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5628..5648)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6046..6057)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6370..7003
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7131..7147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7131..7147)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7144..7166)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7155..7171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7179..7209)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7224..7245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7362..7379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7533..8121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7613..7632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8292..9152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8915..8934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9153..9257)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9305..9439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9342..9361)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9396..9415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggccgccatg gaacaaaaac tcatctcaga agaggatctc
     3421 ggcggatcag ggtcgggtgg ctctggatca ggcggatctg ggtcggggat atcggccggc
     3481 catgacgact atgcggtttt gtccgatact gagctggccg cagtgctacg ccagtacaac
     3541 atccctcatg ggcctattgt gggttccact cgcaagctct acgaaaagaa aatcttcgag
     3601 tacgagaccc agagaaggag actcttaccc cccaactcgt catcttcttc attctcctat
     3661 cagttctcag acttggattc agccgccgtg gactcagaca tgtatgatct gcccaaaaaa
     3721 gaagacgcct tactttacca gagcaaggac tataatgatg actactatga ggagagttat
     3781 ttgaccacca agacatacgg ggagcctgag tctgtgggca tgtccaagag cttccgccag
     3841 ccggggacct cacttgtaga tgctgatacc ttccatcacc aggtgcgtga tgacattttc
     3901 tcttctttag aagaggaagg caaggatagg gagcgcctca tctatggcca agacagtgcc
     3961 taccagagca tcgcacacta ccgccccatt tccaatgtct ccaggagctc tctgggctta
     4021 tcatattatc ctacatcctc cacctcttct gtgtcctcct cttcatcttc tccctcttca
     4081 tggcttaccc gacgtgccat tcggccagag aagcaggccc ctgctgctgc tctgggccag
     4141 gatcgacagg tcccactctg gggccagctg ttactcttcc tggtctttgc tgcctttctg
     4201 ctctttgttt actattccat acaagctgaa gaaggcaacc ccttctggat ggatcccgtt
     4261 taagtttaaa cttcgaaacg cgtgaattcc tcctcgaggg cggccgctct agagtcgacg
     4321 ggccgcggta acaattgtta actaacttaa gctagcaacg gtttccctct agcgggatca
     4381 attccgcccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     4441 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     4501 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     4561 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     4621 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     4681 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     4741 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     4801 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     4861 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     4921 gtggttttcc tttgaaaaac acgataatac catgaccgag tacaagccca cggtgcgcct
     4981 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5041 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5101 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5161 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5221 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5281 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5341 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5401 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5461 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5521 ctggtgcatg acccgcaagc ccggtgcctg acccgggcgg ggcgcgtctg gaacaatcaa
     5581 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5641 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5701 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5761 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5821 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5881 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5941 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6001 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6061 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6121 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca
     6181 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     6241 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     6301 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6361 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     6421 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     6481 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     6541 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     6601 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     6661 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     6721 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     6781 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     6841 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6901 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6961 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7021 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     7081 cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7141 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7201 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7261 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7321 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7381 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7441 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7501 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7561 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7621 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7681 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7741 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7801 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7861 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7921 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7981 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8041 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8101 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8161 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8281 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8341 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8401 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8461 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8521 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8581 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8641 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8701 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8761 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8821 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8881 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8941 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9001 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9061 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9121 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9181 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9241 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     9301 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9361 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9421 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     9481 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     9541 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     9601 agtagt
//
