LOCUS       Exported               10689 bp DNA     circular SYN 29-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Gerace Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3342..3401
                     /label=Fragment.FOR
     regulatory      3382..3390
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3391..3420
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      3391
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    3421..3465
                     /label=3xGSlinker
                     /note="3xGSlinker"
     primer_bind     3421..3437
                     /label=Myc_Bait_F
     primer_bind     3426..3490
                     /label=LBR_TurboID_NEB_F
     primer_bind     complement(3446..3465)
                     /label=V5_TbID__3XGS_R
     misc_feature    3466..5346
                     /label=LBR_ORF_MM
     primer_bind     complement(4596..4615)
                     /label=Lbr Sequencing Primer R
     primer_bind     complement(5318..5389)
                     /label=LBR_TurboID_NEB_R
     primer_bind     5347..5366
                     /label=V5_TbID__3XGS_F
     primer_bind     complement(5352..5374)
                     /label=Myc_Bait_R
     primer_bind     complement(5353..5414)
                     /label=Fragment.REV
     misc_feature    5373..5374
                     /label=IRES
                     /note="/vntifkey=21"
     primer_bind     complement(5627..5644)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5854..5873
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6035..6634
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6035..6054)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6531..6551
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6658..7246
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6711..6731)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7129..7140)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7453..8086
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8214..8230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8214..8230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8227..8249)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8238..8254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8262..8292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8307..8328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8445..8462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8616..9204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8696..8715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9375..10235)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9998..10017
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10236..10340)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10388..10522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10425..10444)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10479..10498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggccgccatg gaacaaaaac tcatctcaga agaggatctc
     3421 ggcggatcag ggtcgggtgg ctctggatca ggcggatctg ggtcgatgcc aagtaggaag
     3481 tttgttgagg gtgaagtggt cagaggccga tggcctggga gctcccttta ttatgaagta
     3541 gagattctga gccacgacaa caaatcccag ctctacactg taaaatacaa agatggcacc
     3601 gaactggaac tgaaggagag tgatatcaag cctttaaaat cctttaagca aagaaaaagt
     3661 ggctcgattt ccagctctcc ctctcgacgc cgtggcagcc gcagccgcag ccgctcaaga
     3721 tcacgttccc gatccccggg cagagcaccg aaaggctcca gaagatccgt ctctgcttcc
     3781 catgagggtg acgtgaagga gaagaaggag aaggagatga ggagggaaat tctgcaagtt
     3841 aagctgactc cgcttgtgtt gaagccgttc ggaaacagcg tcagcgtgta caacggggag
     3901 cccgaacaca tggagaagaa cgctacgccg tataaagaca agcaggaaag aatcattttg
     3961 tcaacagaag acagatatat agttacacag tacagccttc gtcccaggag agaagaggtc
     4021 aaagccaaag aaatagagtc tgaggaacaa aaccttgtca ccaaaggacc cgcacctttg
     4081 ggaacctttc aagtgaccac tccacagagg aaggacttgg agtttggagg agtacctggt
     4141 gcggtcctca tcatgctggg cctgcccgcc tgcgtcctct tgctgctgct gcagtgcagg
     4201 cagaaggacc cggggctgct ccacttccct ccacctctgc cggctctcca cgagctgtgg
     4261 gaacccaggg tgtgcggcgt ttacctcctc tggtttttcg ttcaagctct cttccacctt
     4321 ctaccagttg ggaaggttgc agaaggaaca cctcttgttg atggaagaag actccagtat
     4381 agactgaatg gattgtatgc cttcattctg acatctgcag ccctgggagc agctgtcttc
     4441 tggggtgttg agctgtgcta cctgtacact cacttcctgc agttggcact ggcagccacc
     4501 gggttctccg tgctcctgag tgcttacctc tatgttcgct ctctgcgagc cccgagggag
     4561 gagctgtcgc cagccagttc cggaaatgct gtctacgact tcttcattgg ccgagagtta
     4621 aacccgcgac ttggtgcttt cgatctcaag ttcttttgtg agttgcgccc tggattgatt
     4681 ggatgggtgg ttattaactt agtgatgctt ctgatggaaa tgaaaattca ggagcgagct
     4741 gctccgtcct tggcaatgat cttggttaac agtttccagc tcttatatgt ggtggacgcc
     4801 ctctggaatg aggaagcact gctgacctcc atggacatca tgcacgacgg ctttggcttc
     4861 atgctggcct ttggggattt agtgtgggtt ccatttacct acagcctcca ggccttttac
     4921 ctggtcagcc atccccatga cttgtcctgg ccattggctt ctgtcatcat tgctctgaaa
     4981 ttgtgtggat atgtaatctt ccgttgtgca aactctcaga aaaatgcatt ccggaagaat
     5041 cccactgatc caaagcttgc acatttgaag accattcata cttccacggg gaaaagtctg
     5101 cttgtttccg gatggtgggg ctttgttcgc catcccaatt acttgggtga cctcatcatg
     5161 gctctggcgt ggtccctccc atgtggtttc aaccacctcc tgccatactt ctacattatc
     5221 tacttcaccg ccttgcttat ccatcgagag gcccgggatg aacaccagtg caggaggaag
     5281 tacggcctgg cctgggagaa gtactgccag cgagtgccct accgcatatt cccctacatt
     5341 tactgagttt aaacttcgaa acgcgtgaat tcctcctcga gggcggccgc tctagagtcg
     5401 acgggccgcg gtaacaattg ttaactaact taagctagca acggtttccc tctagcggga
     5461 tcaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     5521 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     5581 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     5641 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     5701 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     5761 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5821 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5881 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     5941 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     6001 gacgtggttt tcctttgaaa aacacgataa taccatgacc gagtacaagc ccacggtgcg
     6061 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6121 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6181 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6241 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6301 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6361 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6421 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6481 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6541 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6601 cacctggtgc atgacccgca agcccggtgc ctgacccggg cggggcgcgt ctggaacaat
     6661 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6721 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6781 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6841 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6901 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6961 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7021 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7081 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7141 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7201 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     7261 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     7321 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     7381 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7441 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     7501 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     7561 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     7621 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     7681 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     7741 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     7801 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     7861 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     7921 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7981 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8041 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     8101 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     8161 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8221 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8281 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8341 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8401 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8821 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8881 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8941 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9001 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9061 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9241 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9301 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9361 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9421 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9481 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9541 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9601 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9661 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9721 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9781 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9841 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9901 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9961 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10021 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10081 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10141 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10201 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10261 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10321 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    10381 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10441 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10501 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    10561 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    10621 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10681 cttagtagt
//
