LOCUS       Exported                9439 bp DNA     circular SYN 18-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Gerace Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="/vntifkey=88"
     promoter        2209..3380
                     /label=EF-1a
                     /note="/vntifkey=29"
     primer_bind     3328..3348
                     /label=EF1a-F
     primer_bind     3391..3430
                     /label=Primer 1
     misc_feature    3391..3401
                     /label=C-term LIC
     misc_feature    3408..3980
                     /label=Cbx5
     primer_bind     complement(3948..3997)
                     /label=Primer 2
     misc_feature    3984..3997
                     /label=C-term LIC
     misc_feature    3990..4007
                     /label=6X Gly
     CDS             4008..4049
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4079..4737
                     /label=IRES
                     /note="/vntifkey=21"
     primer_bind     complement(4258..4277)
                     /label=IRES-R Addgene
     primer_bind     complement(4330..4347)
                     /label=IRES-R Jakki
     CDS             4738..5337
                     /codon_start=1
                     /label=Puro
                     /note="/vntifkey=4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5361..5952
                     /label=WPRE
                     /note="/vntifkey=21"
     CDS             complement(8077..8937)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg ggaaagaaga
     3421 ccaagaggac agccgacagc tcttcttcag aggatgagga ggaatatgtg gtggaaaagg
     3481 tgttggacag gcgcatggtt aaggggcaag tggaatatct gttgaagtgg aaaggctttt
     3541 ctgaggagca caatacttgg gaacctgaga agaacttgga ttgtcctgaa ctaatttctg
     3601 agtttatgaa aaagtataag aagatgaagg agggtgaaaa caataagccc agggagaaat
     3661 cagaaggaaa caagaggaaa tccagtttct ccaacagcgc tgatgatatt aaatctaaaa
     3721 aaaagagaga gcaaagcaat gatatcgctc ggggctttga gagaggactg gaaccagaaa
     3781 agatcatcgg agcaacagat tcctgcggtg acttaatgtt cttaatgaaa tggaaagaca
     3841 cagatgaagc tgacctggtt cttgcaaaag aagctaacgt gaagtgtcca cagattgtga
     3901 tagcatttta tgaagagaga ctgacgtggc acgcatatcc agaggatgcg gaaaacaaag
     3961 aaaaagaaag cgcgaagagc ggtgggccgg gcgggggtgg cgggggaggt aagcctatcc
     4021 ctaaccctct cctcggtctc gattctacgt gagtttaaac ttcgaaacgc gtgaattcct
     4081 cgagggcggc cgctctagag tcgacgggcc gcggtaacaa ttgttaacta acttaagcta
     4141 gcaacggttt ccctctagcg ggatcaattc cgcccccccc ccctaacgtt actggccgaa
     4201 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     4261 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     4321 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     4381 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     4441 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     4501 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     4561 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     4621 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     4681 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg
     4741 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     4801 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     4861 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     4921 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4981 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5041 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5101 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5161 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5221 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5281 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccc
     5341 gggcggggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     5401 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5461 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5521 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5581 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5641 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5701 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     5761 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5821 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5881 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5941 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     6001 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6061 ggtgggtttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     6121 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     6181 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     6241 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     6301 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     6361 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     6421 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     6481 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     6541 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     6601 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6781 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6841 tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta
     6901 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt
     7861 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7921 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7981 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8041 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8101 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8161 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8221 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8281 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8341 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8401 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8461 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8521 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8581 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8641 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8701 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8761 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8821 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8881 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8941 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9001 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9061 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca
     9121 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9181 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     9241 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag
     9301 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     9361 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat
     9421 gtactacaaa cttagtagt
//
