LOCUS       Exported               10644 bp DNA     circular SYN 21-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10644
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="/vntifkey=88"
     promoter        2209..3380
                     /label=EF-1a
                     /note="/vntifkey=29"
     primer_bind     3328..3348
                     /label=EF1a-F
     regulatory      3387..3395
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3438..3455
                     /label=6xGLY
                     /note="6xGLY"
     primer_bind     3447..3481
                     /label=Senp2 N-V5 LIC F
     misc_feature    3447..3458
                     /label=N-term LIC
     misc_feature    3459..5240
                     /label=Senp2
     primer_bind     4134..4153
                     /label=Senp2 N-V5 Sequencing F
     primer_bind     complement(5219..5256)
                     /label=Senp2 N-V5 LIC R
     misc_feature    5245..5256
                     /label=N-term LIC
     misc_feature    5284..5942
                     /label=IRES
                     /note="/vntifkey=21"
     primer_bind     complement(5463..5482)
                     /label=IRES-R Addgene
     primer_bind     complement(5535..5552)
                     /label=IRES-R Jakki
     CDS             5943..6542
                     /codon_start=1
                     /label=Puro
                     /note="/vntifkey=4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6566..7157
                     /label=WPRE
                     /note="/vntifkey=21"
     CDS             complement(9282..10142)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat gtacagatgg ctggctaagg
     3481 ttctcggcac cattcttcgc ttgtgtgagc ggcccgcgcc cggggcccgc gccctactca
     3541 agaggcggcg ctccagcagc actctgtttt ctactgcagt ggacactgat gagatcccag
     3601 ccaaaaggcc aagattagat tgctttattc accaagtgaa gaacagtctc tacaatgctg
     3661 ccagtttatt tggattcccg ttccagctga ccacaaaacc catggtgtct tctgcttgta
     3721 atggaacacg gaatgtggcc ccttctggag aggtcttttc gaactcttca tcttgtgagc
     3781 tgatgagttc tggatcctgc agcagcatgc tgaaactggg taataaatct cctaatggaa
     3841 taagcgacta tccaaagatc agagtgacag tgacccgcga tcagccacgg agagtcctgc
     3901 cctcctttgg ttttacgctg aaatcggaag gctataatag aaggccaagt ggtcgtcgcc
     3961 atagcaaaag caatccagag agttccttaa catggaagcc tcaggagcaa ggtgtgacag
     4021 agatgatttc tgaagagggt ggcaagggtg tgaggcgtcc ccactgcacc gtggaggagg
     4081 gtgtccaaaa ggatgaaaga gaaaagtatc gaaagttatt ggagcgactt aaagaaggtg
     4141 ctcatggaag cacgttccct cccacggttt cacaccacag ttctcagaga attcagatgg
     4201 acacattaaa gaccaaaggc tgggtggagg agcagaatca tggcgtcaga acaacccatt
     4261 ttgttccaaa acagtataga gttgttgaaa caagaggacc tctctgttca atgagaagtg
     4321 aaaagaggta ttcaaaaggg aaagctgata cagagaaggt ggtgggactc cgatttgaaa
     4381 aggaaggtac aagaggacac caaatggagc ctgatctgtc agaagaagtg tctgcccgac
     4441 ttcgtctagg aagtggaagc aatggcttac tcagaaggaa aatatcagtg cttgagataa
     4501 aggaaaagaa cttcccgagc aaagagaagg acagaagaac ggaagacctc tttgaattta
     4561 cagagcatcg tctaattcag gacatggaaa aggaaatcag taatgccctg ggccacggcc
     4621 caccagatga gattttgagc agcgctttca agttgcgaat cacccgcgga gacatccaga
     4681 ccttaaagaa ctatcattgg ctcaacgatg aggtcattaa tttttatatg aatcttttag
     4741 tggaaaggag taaaaagcaa ggttatccag cacttcatgc attcagtaca ttcttctatc
     4801 ctaagctaaa gtctggaggt taccaagcag tgaaaagatg gaccaaaggg gtaaatctct
     4861 tcgaacaaga acttgttctg gtacctattc atcggaaggt acattggagc ctggtggtga
     4921 tggacctaag aaaaaagtgt cttaaatatc tggattctat gggacaaaaa ggccacagga
     4981 tctgtgagat tcttcttcag tatttacagg atgaaagcaa gaccaaaaga aatacagatc
     5041 tcaacctctt agagtggacc cactacagca tgaagccaca tgagattcct cagcagttga
     5101 atgggagtga ctgtggaatg tttacttgta aatatgcaga ttatatttct agggacaaac
     5161 ctatcacatt cactcagcac cagatgcctc tcttccggaa gaagatggtg tgggagatcc
     5221 ttcaccagca gttgctgtga taatgggccg cgacgatgtt taaacttcga aacgcgtgaa
     5281 ttcctcgagg gcggccgctc tagagtcgac gggccgcggt aacaattgtt aactaactta
     5341 agctagcaac ggtttccctc tagcgggatc aattccgccc ccccccccta acgttactgg
     5401 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     5461 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     5521 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     5581 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     5641 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     5701 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     5761 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     5821 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     5881 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
     5941 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     6001 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     6061 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     6121 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     6181 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     6241 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     6301 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     6361 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     6421 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6481 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     6541 gacccgggcg gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     6601 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6661 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6721 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6781 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6841 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6901 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6961 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7021 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7081 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7141 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     7201 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7261 gaggaggtgg gtttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     7321 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     7381 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     7441 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     7501 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     7561 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     7621 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     7681 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     7741 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     7801 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7861 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7921 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7981 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8041 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct
     8101 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8161 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8221 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9061 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9121 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9181 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9241 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9301 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9361 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9421 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9481 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9541 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9601 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9661 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9721 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9781 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9841 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9901 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9961 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10021 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10081 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10141 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10201 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10261 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
    10321 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    10441 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    10501 cctagtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10561 attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta
    10621 aatatgtact acaaacttag tagt
//
