LOCUS       Exported               10030 bp DNA     circular SYN 21-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Gerace Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="/vntifkey=88"
     promoter        2209..3380
                     /label=EF-1a
                     /note="/vntifkey=29"
     primer_bind     3328..3348
                     /label=EF1a-F
     primer_bind     3391..3429
                     /label=Primer 1
     misc_feature    3391..3401
                     /label=C-term LIC
     misc_feature    3408..4571
                     /label=Rbmxl1
     primer_bind     complement(4544..4588)
                     /label=Primer 2
     misc_feature    4575..4588
                     /label=C-term LIC
     misc_feature    4581..4598
                     /label=6X Gly
     CDS             4599..4640
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4670..5328
                     /label=IRES
                     /note="/vntifkey=21"
     primer_bind     complement(4849..4868)
                     /label=IRES-R Addgene
     primer_bind     complement(4921..4938)
                     /label=IRES-R Jakki
     CDS             5329..5928
                     /codon_start=1
                     /label=Puro
                     /note="/vntifkey=4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5952..6543
                     /label=WPRE
                     /note="/vntifkey=21"
     CDS             complement(8668..9528)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg gttgaagcag
     3421 atcgcccagg aaagctcttc attggtgggc ttaatacaga aacaaatgag aaagcccttg
     3481 aagcagtatt tggcaagtat ggacgaatag tggaaatact tttgatgaaa gaccgggaaa
     3541 ccaacaaatc aagaggattt gcttttgtta ccttcgaaag cccagcagat gctaaagatg
     3601 cagctagaga tatgaatgga aagtccttgg atggaaaagc catcaaggtg gagcaagcta
     3661 ccaaaccatc ttttgaaagt ggcaggcgtg gaccacctcc acctccaaga agcaggggcc
     3721 ctcccagagg tcttcgagga ggaagtggag gaactagggg acccccttca cgtggaggat
     3781 acatggatga tggtggttat tccatgaact ttaacatgag ttcttccagg ggaccacttc
     3841 cagtaaagag aggaccacca ccacgaagcg ggggtccccc tcctaaaaga tcaacacctt
     3901 ccggaccagt tcgaagcagc agtggaatgg gcggaagaac accagtgtcc cgtggaagag
     3961 atagctacgg gggtccacca cgaagggaac ccctgccatc tcgcagagat gtttatttgt
     4021 ccccaagaga tgatggatat tctacaaaag atagctattc aagcagagat tacccaagct
     4081 cccgagacac cagagattat gcaccgccac caagagatta tacttaccgt gattacagtc
     4141 attccagttc ccgtgatgac tatccatcaa gaggctacgg tgatagagat ggatatggtc
     4201 gggatcgtga ctattcagat catccaagtg gaggttccta cagagattca tatgagagtt
     4261 atggaaactc acgcagtgca ccccctacac gagggccacc accatcttat ggaggaagca
     4321 gtcgctatga tgattacagc agctcacgtg atggctacgg tggaagtcga gacagttact
     4381 caagcagcag aagtgatctc tactcaagtg gccgtgatcg agttggcaga caagaacgag
     4441 ggcttccccc ttctatggaa agggggtacc ctcctccacg tgattcctac agcagttcaa
     4501 gccgaggagc gccaagagga ggtggccgtg gagggagccg atctgataga gggggaggca
     4561 gaagcagata tggtgggccg ggcgggggtg gcgggggagg taagcctatc cctaaccctc
     4621 tcctcggtct cgattctacg tgagtttaaa cttcgaaacg cgtgaattcc tcgagggcgg
     4681 ccgctctaga gtcgacgggc cgcggtaaca attgttaact aacttaagct agcaacggtt
     4741 tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg
     4801 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     4861 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     4921 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     4981 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     5041 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     5101 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     5161 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     5221 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     5281 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat gaccgagtac
     5341 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5401 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5461 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5521 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5581 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5641 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5701 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     5761 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     5821 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5881 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc cgggcggggc
     5941 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6001 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6061 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6121 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6181 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6241 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6301 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6361 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6421 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6481 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6541 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6601 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6661 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     6721 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     6781 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     6841 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     6901 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     6961 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     7021 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     7081 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     7141 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     7201 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7261 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7321 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7381 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7441 gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc
     7501 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     8461 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8521 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8581 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8641 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8701 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8761 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8821 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8881 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8941 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9001 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9061 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9121 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9181 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9241 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9301 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9361 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9421 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9481 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9541 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9601 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9661 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
     9721 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9781 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     9841 atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat
     9901 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     9961 atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata tgtactacaa
    10021 acttagtagt
//
