LOCUS       Exported               10234 bp DNA     circular SYN 25-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Liza Brusman
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="/vntifkey=88"
     promoter        2209..3380
                     /label=EF-1a
                     /note="/vntifkey=29"
     primer_bind     3328..3348
                     /label=EF1a-F
     primer_bind     3391..3433
                     /label=Gpat4 C-V5 LIC F
     misc_feature    3391..3401
                     /label=C-term LIC
     CDS             3408..4775
                     /codon_start=1
                     /label=Gpat4
                     /translation="MFLLLPFDSLIVNLLGISLTVLFTLLLVFIIVPAIFGVSFGIRKL
                     YMKTLLKIFAWATLRMERGAKERNHQLYKPYTNGIIAKDPTSLEEEIKEIRRSGSSKAL
                     DKTPEFELSDIFYFCRKGMETIMDDEVTKRFSAEELESWNLLSRTNYNFQYISLRLTIL
                     WGLGVLIRYCFLLPLRIALAFTGIGLLVVGTTMVGYLPNGRFKEFLSKHVHLMCYRICV
                     RALTAIITYHNRKNRPRNGGICVANHTSPIDVIILASDGYYAMVGQVHGGLMGVIQRAM
                     VKACPHVWFERSEVKDRHLVAKRLTEHVQDKSKLPILIFPEGTCINNTSVMMFKKGSFE
                     IGATVYPVAIKYDPQFGDAFWNSSKYGMVTYLLRMMTSWAIVCSVWYLPPMTREKDEDA
                     VQFANRVKSAIARQGGLVDLLWDGGLKREKVKDTFKEEQQKLYSKMIVGNHEDRSRS"
     primer_bind     complement(4761..4792)
                     /label=Gpat4 C-V5 LIC R
     misc_feature    4779..4792
                     /label=C-term LIC
     misc_feature    4785..4802
                     /label=6X Gly
     CDS             4803..4844
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4874..5532
                     /label=IRES
                     /note="/vntifkey=21"
     primer_bind     complement(5053..5072)
                     /label=IRES-R Addgene
     primer_bind     complement(5125..5142)
                     /label=IRES-R Jakki
     CDS             5533..6132
                     /codon_start=1
                     /label=Puro
                     /note="/vntifkey=4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6156..6747
                     /label=WPRE
                     /note="/vntifkey=21"
     CDS             complement(8872..9732)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg ttcctgttgc
     3421 taccttttga tagcttgatt gtcaacctcc tgggtatctc cctgaccgtc ctcttcaccc
     3481 tgctgcttgt tttcatcatc gtccctgcca tctttggagt gtcctttggt atccgaaagc
     3541 tctacatgaa aacactgcta aagatctttg cgtgggccac cctgagaatg gagagaggag
     3601 ccaaggagag gaaccaccag ctgtacaagc cctacaccaa cggaatcatt gcaaaagatc
     3661 ccacttctct ggaagaagaa atcaaagaaa ttcgtcgaag tggtagtagt aaagctctgg
     3721 ataagacacc cgagttcgag ctctctgaca tcttctactt ttgccggaaa ggaatggaga
     3781 ctatcatgga tgatgaagtg acaaagagat tctcggcaga ggagctggag tcctggaact
     3841 tgctgagcag aaccaattac aacttccaat acataagcct gcggcttacc atcctctggg
     3901 gcttaggagt gctgattcgg tattgcttcc tcctgccact caggattgcg ctggcattca
     3961 cggggattgg cctcttggta gtgggaacta ctatggttgg atacctgcca aatgggagat
     4021 ttaaggagtt tttgagtaaa cacgttcact taatgtgcta ccgtatctgt gtgcgagccc
     4081 tgacggccat cattacgtac cacaacagga aaaacagacc aagaaatggt ggcatctgtg
     4141 tggctaacca tacatccccc attgatgtga tcatcttggc cagcgacggc tactacgcca
     4201 tggtgggaca ggttcacggg ggccttatgg gtgtgattca gagagcaatg gtgaaagcct
     4261 gcccccatgt ctggtttgag cgttctgagg tgaaagatcg ccacctggtg gctaagaggc
     4321 tgactgagca tgtccaggat aaaagcaagc tgcccatcct catcttccca gaaggaacct
     4381 gcatcaataa cacatcagtg atgatgttca agaagggaag ctttgaaatt ggagccactg
     4441 tttaccctgt ggctatcaag tatgaccctc agtttggtga cgccttctgg aacagcagca
     4501 agtatggcat ggtgacgtac cttctgagga tgatgaccag ttgggccatt gtctgcagcg
     4561 tttggtacct gcctcctatg actcgagaga aagatgaaga tgctgtgcag tttgctaaca
     4621 gagtgaagtc tgccattgcc cggcagggag gattggtaga cctgctgtgg gacggtggat
     4681 tgaagagaga aaaggtgaag gacacattca aggaggagca gcagaagcta tatagcaaga
     4741 tgatagtcgg aaaccatgag gaccgcagcc ggtccggtgg gccgggcggg ggtggcgggg
     4801 gaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtgagtt taaacttcga
     4861 aacgcgtgaa ttcctcgagg gcggccgctc tagagtcgac gggccgcggt aacaattgtt
     4921 aactaactta agctagcaac ggtttccctc tagcgggatc aattccgccc ccccccccta
     4981 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     5041 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     5101 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     5161 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     5221 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     5281 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     5341 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     5401 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     5461 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     5521 cacgataata ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5581 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5641 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5701 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5761 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5821 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5881 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5941 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6001 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6061 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6121 cccggtgcct gacccgggcg gggcgcgtct ggaacaatca acctctggat tacaaaattt
     6181 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6241 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6301 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6361 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6421 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6481 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6541 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6601 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6661 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6721 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     6781 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     6841 acaagaggag gaggaggtgg gtttttccag tcacacctca ggtaccttta agaccaatga
     6901 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     6961 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7021 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     7081 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     7141 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7201 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     7261 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     7321 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     7381 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     7441 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7501 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7561 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7621 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc
     7681 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7741 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7801 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7861 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7921 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7981 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8041 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8101 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8161 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8221 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8281 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8341 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8401 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8461 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8521 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8581 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8641 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8701 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8761 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8821 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8881 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8941 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9001 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9061 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9841 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
     9901 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9961 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    10021 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    10081 ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga attattttca
    10141 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt
    10201 gtatttgtta aatatgtact acaaacttag tagt
//
