LOCUS       Exported                9700 bp DNA     circular SYN 25-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Liza Brusman
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="/vntifkey=88"
     promoter        2209..3380
                     /label=EF-1a
                     /note="/vntifkey=29"
     primer_bind     3328..3348
                     /label=EF1a-F
     primer_bind     3391..3416
                     /label=Agpat2 C-V5 LIC F
     misc_feature    3391..3401
                     /label=C-term LIC
     CDS             3408..4241
                     /codon_start=1
                     /label=Agpat2
                     /translation="MDPWPWLTAALLLLLLLVQLSRTARFYAKVGLYCVLCLSFSAAAS
                     IVCLLRHGGRTVDNMSIISWFVRSFKYVYGLRFEVSGQKKLEVDGPCVIISNHQSILDM
                     MGLMEILPKRCVQIAKRELMFTGPVGLIMYLGGVYFINRQQARTAMSVMADLGDLMVKE
                     NLKVWIYPEGTRNDNGDLLPFKKGAFYLAIQAQVPIIPVVYSSFSSFYNVKTKLFTSGT
                     IKVQVLDAVPTNGLTDADVTKLVDTCYQSMRATFLQISQIPQENSAIKEPGVLPAQ"
     primer_bind     complement(4218..4258)
                     /label=Agpat2 C-V5 LIC R
     misc_feature    4245..4258
                     /label=C-term LIC
     misc_feature    4251..4268
                     /label=6X Gly
     CDS             4269..4310
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4340..4998
                     /label=IRES
                     /note="/vntifkey=21"
     primer_bind     complement(4519..4538)
                     /label=IRES-R Addgene
     primer_bind     complement(4591..4608)
                     /label=IRES-R Jakki
     CDS             4999..5598
                     /codon_start=1
                     /label=Puro
                     /note="/vntifkey=4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5622..6213
                     /label=WPRE
                     /note="/vntifkey=21"
     CDS             complement(8338..9198)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg gacccgtggc
     3421 catggctgac ggcggcgctg ctgctgctgt tgctgcttgt gcagctgagc cgcacagcca
     3481 ggttctacgc caaggtcggt ctctactgcg tgctctgcct gtccttctcc gccgcggcct
     3541 cgatcgtctg cctgctgcgc cacggcggcc gcaccgtgga taacatgagc atcatcagct
     3601 ggttcgttcg gtccttcaag tacgtgtatg gccttcgctt tgaggtcagc ggacagaaga
     3661 aactggaggt ggatggtccc tgtgtcatca tctctaatca ccagagcatc ctggacatga
     3721 tgggtctcat ggaaatactc cctaagcgct gtgtgcagat cgccaagcgt gagctaatgt
     3781 tcacagggcc tgtgggcctc atcatgtacc ttgggggtgt ctacttcatc aaccgccagc
     3841 aagccagaac tgccatgtct gtgatggccg acctgggcga cctcatggtc aaggagaatc
     3901 tcaaagtgtg gatctaccca gagggtacac gcaacgacaa tggggacctg ttgcccttta
     3961 aaaaaggagc cttctacttg gccatccagg cccaggtgcc catcatcccc gtggtgtact
     4021 cgtctttctc ttccttctac aatgtcaaga cgaagctctt cacctcagga acaatcaagg
     4081 tacaagtgct ggatgctgtc cctaccaatg gtctgacaga tgctgatgtc accaagctgg
     4141 tggacacttg ctaccagtcc atgagggcca cctttctaca gatttctcag attccccaag
     4201 agaactctgc cattaaggag cctggggtct tgccagccca gggtgggccg ggcgggggtg
     4261 gcgggggagg taagcctatc cctaaccctc tcctcggtct cgattctacg tgagtttaaa
     4321 cttcgaaacg cgtgaattcc tcgagggcgg ccgctctaga gtcgacgggc cgcggtaaca
     4381 attgttaact aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc
     4441 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4501 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4561 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4621 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     4681 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     4741 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     4801 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     4861 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     4921 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     4981 gaaaaacacg ataataccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5041 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5101 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5161 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5221 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5281 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5341 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5401 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5461 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     5521 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     5581 cgcaagcccg gtgcctgacc cgggcggggc gcgtctggaa caatcaacct ctggattaca
     5641 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5701 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5761 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5821 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5881 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5941 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6001 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6061 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6121 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6181 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
     6241 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     6301 agaagcacaa gaggaggagg aggtgggttt ttccagtcac acctcaggta cctttaagac
     6361 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
     6421 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     6481 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     6541 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     6601 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     6661 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     6721 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     6781 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     6841 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     6901 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6961 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7021 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7081 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
     7141 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7201 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7261 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7321 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7381 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7441 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7501 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7561 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7741 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7981 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8101 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8581 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8641 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8701 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8761 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8821 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8881 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8941 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9001 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9061 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9301 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta
     9361 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9421 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9481 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     9541 ttaccactgc caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta
     9601 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa
     9661 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
