LOCUS       Exported               10492 bp DNA     circular SYN 09-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Liza Brusman
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="/vntifkey=88"
     promoter        2209..3380
                     /label=EF-1a
                     /note="/vntifkey=29"
     primer_bind     3328..3348
                     /label=EF1a-F
     primer_bind     3391..3423
                     /label=Pigb C-V5 LIC F
     misc_feature    3391..3401
                     /label=C-term LIC
     CDS             3408..5033
                     /codon_start=1
                     /label=Pigb
                     /translation="MESQAADYNPASRNLHGSSGEMKLRRRKSRQYVSAQEKRSPRRGL
                     LGENTYLVLFTIALRILNCFLVQTSFVPDEYWQSLEVAHRMVFSYGYLTWEWTERLRGY
                     TYPLIFASIYKVLHLLGKDSVQFLIWIPRLGQALLSAVADIRLYSLLKQLENQEVAQWV
                     FLCQLCSWFTWYCCTRTLTNTMETSLTALALFYYPLEGSRSVNSVKYSLLVALACVVRP
                     TALIPWVPLLFRHFYQEQRKLHLTLHHFLPVGFITFSLSLIIDRIFFGQWTLVQLNFLK
                     FNVLQNLGTFYGSHPWHWYLSQGFPVVLGTHLPFFIHGCFLAPRRLHILLLTVLWTLLV
                     YSMLGHKEFRFIYPVLPFCMVFCGYSLAHLKTWRKAALSFLLLSNVPLAFYTGLVHQRG
                     TLDVMNHIQKVCPRGPDPASASVFIMMPCHSTPYYSHVHCPLSMRFLQCPPDLTGKTQY
                     LDEADMFYLNPLRWLQQEFHSNASLPTHLVTFNVLEKEINTFLTSGNYERAATFFHTHW
                     PERRTGSHIHVYERRLPGRVNTGGN"
     primer_bind     complement(5012..5050)
                     /label=Pigb C-V5 LIC R
     misc_feature    5037..5050
                     /label=C-term LIC
     misc_feature    5043..5060
                     /label=6X Gly
     CDS             5061..5102
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5132..5790
                     /label=IRES
                     /note="/vntifkey=21"
     primer_bind     complement(5311..5330)
                     /label=IRES-R Addgene
     primer_bind     complement(5383..5400)
                     /label=IRES-R Jakki
     CDS             5791..6390
                     /codon_start=1
                     /label=Puro
                     /note="/vntifkey=4"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6414..7005
                     /label=WPRE
                     /note="/vntifkey=21"
     CDS             complement(9130..9990)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg gagtcccagg
     3421 ccgccgacta taacccggcc tcccgcaatc tccacggcag ctcgggagaa atgaagctgc
     3481 ggaggaggaa gtccaggcag tacgtgagcg cgcaggagaa acgctctccg cgccgtggtc
     3541 ttcttggaga aaacacttac ctggtcttgt ttaccatcgc tctgcgaata cttaactgct
     3601 tcttagtgca gacaagcttt gttcctgatg aatactggca gtccctcgaa gttgcacacc
     3661 gcatggtttt cagttatggt tatttgacct gggaatggac ggagcgattg agaggctaca
     3721 cttaccccct catcttcgcc agtatttaca aggttctgca tcttctgggg aaagacagtg
     3781 ttcaattcct gatctggatt cccagacttg gccaagctct tctgtctgct gtagcggata
     3841 taaggctcta ctccctcctg aagcagctgg aaaaccagga agtggcacaa tgggtgttcc
     3901 tctgccagct gtgctcctgg ttcacatggt actgctgcac acggaccctc acgaacacca
     3961 tggagacctc cctcactgcc ctcgcccttt tctactatcc tctggaaggc tcgaggtccg
     4021 tgaacagcgt caagtactcg ctcctggtgg cacttgcttg tgtggtccgc cccacagctc
     4081 ttatcccatg ggtgccgctg ctcttcagac atttctacca agaacagaga aagctccatc
     4141 tgactctgca tcatttttta cctgtaggat ttataacctt cagtttgtct ctgataattg
     4201 accgtatctt ttttggccaa tggacgctgg tccaattaaa tttcttgaaa tttaacgtgc
     4261 tgcagaacct agggacgttt tatggctccc atccatggca ctggtacctc agtcaagggt
     4321 tcccagttgt cctggggacc cacttacctt tcttcattca cggttgcttc ctagccccaa
     4381 ggaggctcca catactgctg ctgaccgtgc tgtggacact gctggtatac agcatgttgg
     4441 gccacaaaga attcaggttt atctatccag ttttaccatt ctgcatggtg ttctgtggtt
     4501 actcactagc ccacctgaag acatggagga aggcagccct gagcttcctg ctcttgtcaa
     4561 atgtgccact ggcattctac actggcctag ttcatcagag aggcaccctg gatgtcatga
     4621 atcacattca gaaagtctgc ccccgaggtc ctgacccagc ttcggcctca gtattcataa
     4681 tgatgccctg ccactccacg ccttactaca gccatgttca ctgcccactg tccatgcggt
     4741 ttctccagtg tcccccagac ttgactggaa aaactcagta tcttgatgaa gcagatatgt
     4801 tctacttaaa ccccttaaga tggttacaac aagaatttca cagcaatgcg tcactcccca
     4861 ctcacttggt caccttcaat gttctggaga aggagatcaa taccttcctc acctcaggga
     4921 actacgagag ggccgctaca ttcttccaca ctcactggcc agagcgccga actggaagtc
     4981 acatacacgt ctacgagcga aggttacccg gcagggtcaa cacaggaggg aacggtgggc
     5041 cgggcggggg tggcggggga ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     5101 cgtgagttta aacttcgaaa cgcgtgaatt cctcgagggc ggccgctcta gagtcgacgg
     5161 gccgcggtaa caattgttaa ctaacttaag ctagcaacgg tttccctcta gcgggatcaa
     5221 ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5281 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5341 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5401 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5461 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5521 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5581 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5641 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5701 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5761 tggttttcct ttgaaaaaca cgataatacc atgaccgagt acaagcccac ggtgcgcctc
     5821 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     5881 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     5941 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6001 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6061 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6121 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6181 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6241 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6301 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6361 tggtgcatga cccgcaagcc cggtgcctga cccgggcggg gcgcgtctgg aacaatcaac
     6421 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6481 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6541 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6601 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6661 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6721 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6781 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     6841 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6901 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6961 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
     7021 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     7081 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt ttttccagtc acacctcagg
     7141 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7201 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     7261 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     7321 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     7381 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     7441 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     7501 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     7561 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     7621 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7681 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7741 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7801 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7861 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     7921 cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     7981 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8041 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8101 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8161 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8221 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8281 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8341 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8401 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8461 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8521 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8581 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8641 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8701 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8761 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8821 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8881 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg
     8941 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9001 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9061 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9121 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9181 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9241 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9301 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9361 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9421 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9481 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9541 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9601 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9661 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9721 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9781 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9841 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9901 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9961 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10021 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10081 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    10141 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10201 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10261 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    10321 ccccataccc tattaccact gccaattacc tagtggtttc atttactcta aacctgtgat
    10381 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    10441 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
