LOCUS       Exported               10953 bp DNA     circular SYN 12-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10953
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    330..989
                     /label=AMPICILIN
                     /note="AMPICILIN"
     misc_feature    4012..4129
                     /label=cPPT
                     /note="cPPT"
     misc_feature    4152..4678
                     /label=CMV promoter
                     /note="CMV promoter"
     misc_feature    4737..7829
                     /label=mCherry-linker-DDX1_18F151R PCR product
     misc_feature    4737..7829
                     /label=DNA*_cloneInsert 1
     misc_feature    4737..4769
                     /label=HY18F
                     /note="HY18F"
     CDS             4755..5462
                     /label=mCherry
     misc_feature    5463..5492
                     /label=GS linker
                     /note="GS linker"
     misc_feature    5493
                     /label=HY_F primer to clone DDX1 into pCR2.1
                     /note="HY_F primer to clone DDX1 into pCR2.1 vector"
     misc_feature    5494..7721
                     /label=TagRFP-linker-DDX1 in pcDNA3.1
     misc_feature    5499..7718
                     /label=DDX1
                     /note="DDX1"
     misc_feature    7719..7720
                     /label=Add
                     /note="Add ""GC"" to avoid frameshift"
     misc_feature    7741..7790
                     /label=V5 Epitope
                     /note="V5 Epitope"
     misc_feature    7800..7817
                     /label=Poly His Tag
                     /note="Poly His Tag"
     misc_feature    7803..7829
                     /label=HY151R
                     /note="HY151R"
     misc_feature    8957..9556
                     /label=PURO
                     /note="PURO"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
     4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     4681 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctagaggatc
     4741 caccggtgcc aaccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     4801 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     4861 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     4921 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     4981 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     5041 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     5101 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     5161 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     5221 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     5281 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     5341 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     5401 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     5461 agggcggagg tgggtcgggt ggcggcggat cagctagcat ggcggccttc tccgagatgg
     5521 gtgtaatgcc tgagattgca caagctgtgg aagagatgga ttggctcctc ccaactgata
     5581 tccaggctga atctatccca ttgatcttag gaggaggtga tgtacttatg gctgcagaaa
     5641 caggaagtgg caaaactggt gcttttagta ttccagttat ccagatagtt tatgaaactc
     5701 tgaaagacca acaggaaggc aaaaaaggaa aaacaacaat taaaactggt gcttcagtgc
     5761 tgaacaaatg gcagatgaac ccatatgaca gaggatctgc ttttgcaatt gggtcagatg
     5821 gtctttgttg tcaaagcaga gaagtaaagg aatggcatgg gtgtagagct actaaaggat
     5881 taatgaaagg gaaacactac tatgaagtat cctgtcatga ccaagggtta tgcagggtcg
     5941 ggtggtctac catgcaggcc tctttggacc taggtactga caagtttgga tttggctttg
     6001 gtggaacagg aaagaaatcc cataacaaac aatttgataa ttatggagag gaattcacta
     6061 tgcatgatac cattggatgt tacctggata tagataaggg acatgtcaag ttctccaaaa
     6121 atggaaaaga tcttggtctg gcatttgaaa taccaccaca tatgaaaaac caagccctct
     6181 ttcctgcctg tgttttgaag aatgctgaac tgaaatttaa cttcggtgaa gaggaattta
     6241 agtttccacc aaaagatggc tttgttgctc tttccaaggc accggatggt tacattgtca
     6301 aatcacagca ctcaggtaat gcacaggtga cacaaacaaa gtttctcccc aatgctccga
     6361 aagctctcat tgttgaacct tcccgggagt tagctgaaca aactttgaac aacatcaagc
     6421 agtttaagaa atacattgat aatcctaaat taagggagct tctgataatt ggaggtgttg
     6481 cagcccggga tcagctctct gttttggaaa atggagtaga tatagttgta ggtactccgg
     6541 gaagactaga tgacttggtg tcaactggaa agctgaactt atctcaagtt agattcctgg
     6601 tcctggatga agctgatggg cttctttctc aaggttattc tgattttata aataggatgc
     6661 acaatcagat tcctcaggtt acctctgatg gaaaaagact tcaggtgatt gtttgctctg
     6721 ccactttgca ttctttcgat gtaaagaaac tgtccgagaa gataatgcat tttcctacat
     6781 gggttgactt aaaaggagaa gactctgttc cagatactgt acaccatgtt gttgtcccag
     6841 taaatcccaa aactgacaga ctctgggaaa ggcttggaaa gagccacatt agaactgatg
     6901 atgtacatgc aaaagataac acaagacctg gtgctaatag tccagagatg tggtctgaag
     6961 ctattaaaat cctgaaaggg gagtatgctg tccgggcaat caaggaacat aagatggatc
     7021 aagcaattat cttctgtaga accaaaattg actgtgataa cttggagcag tactttatac
     7081 aacaaggagg aggacctgat aaaaaaggac accagttctc atgtgtttgt cttcatggtg
     7141 acagaaagcc tcatgagaga aagcaaaact tggaaagatt taagaaagga gatgtaagat
     7201 tcttgatttg cacagatgta gctgctagag gaattgatat ccacggtgtt ccttatgtta
     7261 taaatgtcac tctgcccgat gaaaagcaaa actacgtaca tcgaattggc agagtaggaa
     7321 gagctgaaag gatgggtctg gcaatttccc tggtggcaac agaaaaagaa aaggtttggt
     7381 accatgtatg tagcagccgt ggaaaagggt gttataacac aagactcaag gaagatggag
     7441 gctgtaccat atggtacaac gagatgcagt tactatctga gatagaagaa cacctgaact
     7501 gtaccatttc tcaggttgag ccggatataa aggtaccagt ggatgaattt gatgggaaag
     7561 ttacctacgg tcagaaaagg gctgctggtg gtggaagcta taaaggccat gtggatattt
     7621 tggcacctac tgttcaagag ttggctgccc ttgaaaagga ggcgcagaca tctttcctgc
     7681 atcttggcta ccttcctaac cagctgttca gaaccttcgc ctcgagtcta gagggcccgc
     7741 ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc
     7801 atcatcacca tcaccattga gtttaaacgt cgacaatcaa cctctggatt acaaaatttg
     7861 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7921 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7981 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     8041 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8101 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8161 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8221 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     8281 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8341 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8401 ctccctttgg gccgcctccc cgcctggaat tctaccgggt aggggaggcg cttttcccaa
     8461 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     8521 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     8581 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     8641 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     8701 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     8761 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     8821 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     8881 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     8941 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     9001 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     9061 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     9121 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     9181 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     9241 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     9301 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     9361 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     9421 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     9481 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     9541 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     9601 accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag
     9661 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa
     9721 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9781 agctctctgg ctgcctaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9841 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9901 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     9961 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
    10021 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10081 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
    10141 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    10201 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10261 cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg
    10321 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    10381 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    10441 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg
    10501 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10561 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10621 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10681 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10741 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10801 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10861 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10921 aattttaaca aaatattaac gtttacaatt tcc
//
