LOCUS       Exported                6624 bp ds-DNA     circular SYN 13-AUG-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV hSyn FLEX iGluSnFR3 v857.GPI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   HHMI-Janelia
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 13, 2021 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(19..40)
                     /label=f.AAV backbone seq.01.61
                     /note="f.AAV backbone seq.01.61"
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     complement(19..40)
                     /label=f.AAV backbone seq.01.61
                     /note="color: black; sequence: TCCAACCCGGTAAGACACGACT; 
                     added: 2021-01-23"
     misc_feature    complement(124..147)
                     /label=f.AAV backbone seq.01.60
                     /note="f.AAV backbone seq.01.60"
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     complement(124..147)
                     /label=f.AAV backbone seq.01.60
                     /note="color: black; sequence: GTAGGTATCTCAGTTCGGTGTAGG; 
                     added: 2021-01-23"
     misc_feature    308..328
                     /label=f.ITR for 1.01.13
                     /note="f.ITR for 1.01.13"
                     /note="color: #ff8000"
     primer_bind     308..328
                     /label=f.ITR for 1.01.13
                     /note="color: black; sequence: GCGTCGATTTTTGTGATGCTC; 
                     added: 2021-01-23"
     misc_feature    406..429
                     /label=f.ITR for 2.01.14
                     /note="f.ITR for 2.01.14"
                     /note="color: #ff8000"
     primer_bind     406..429
                     /label=f.ITR for 2.01.14
                     /note="color: black; sequence: CTCACATGTTCTTTCCTGCGTTAT; 
                     added: 2021-01-23"
     misc_feature    593..757
                     /label=ITR
                     /note="ITR"
                     /note="color: #ffff00"
     primer_bind     complement(841..861)
                     /label=f.ITR for 4.01.16
                     /note="color: black; sequence: ATTTTATGTTTCAGGTTCAGG; 
                     added: 2021-01-23"
     primer_bind     complement(866..883)
                     /label=r.sv40 seq.01.69
                     /note="color: black; sequence: GTTAACAACAACAATTGC; added: 
                     2021-01-23"
     primer_bind     908..928
                     /label=r.AAV sv40 seq rev.01.5
                     /note="color: black; sequence: TACAAATAAAGCAATAGCATC; 
                     added: 2021-01-23"
     misc_feature    complement(977..1000)
                     /label=f.ITR for 3.01.15
                     /note="f.ITR for 3.01.15"
                     /note="color: #21ffff; direction: LEFT"
     primer_bind     complement(977..1000)
                     /label=f.ITR for 3.01.15
                     /note="color: black; sequence: AAGATACATTGATGAGTTTGGACA; 
                     added: 2021-01-23"
     misc_feature    complement(1052..1666)
                     /label=wpre
                     /note="wpre"
                     /note="color: #ffff00; direction: LEFT"
     primer_bind     complement(1111..1129)
                     /label=f.WPRE seq.01.81
                     /note="color: black; sequence: CTTCGCCTTCGCCCTCAGA; added: 
                     2021-01-23"
     primer_bind     1112..1131
                     /label=r.wpre seq.01.55
                     /note="r.wpre seq.01.55"
                     /note="color: #ff8080; direction: RIGHT"
     primer_bind     1112..1131
                     /label=r.wpre seq.01.55
                     /note="color: black; sequence: CTGAGGGCGAAGGCGAAGAC; added:
                     2021-01-23"
     primer_bind     complement(1222..1241)
                     /label=f.WPRE seq.01.80
                     /note="color: black; sequence: TGTTGCCACCTGGATTCTGC; added:
                     2021-01-23"
     primer_bind     1245..1265
                     /label=r.wpre pcr rev.32.63
                     /note="r.wpre pcr rev.32.63"
                     /note="color: #ff8080; direction: RIGHT"
     primer_bind     complement(1401..1419)
                     /label=f.wpre seq.01.50
                     /note="f.wpre seq.01.50"
                     /note="color: #ff8080; direction: LEFT"
     primer_bind     1401..1419
                     /label=r.wpre seq.01.71
                     /note="r.wpre seq.01.71"
                     /note="color: #ff8080; direction: RIGHT"
     primer_bind     complement(1401..1419)
                     /label=f.wpre seq.01.50
                     /note="color: black; sequence: GCATTGCCACCACCTGTCA; added: 
                     2021-01-23"
     primer_bind     1401..1419
                     /label=r.wpre seq.01.71
                     /note="color: black; sequence: TGACAGGTGGTGGCAATGC; added: 
                     2021-01-23"
     primer_bind     1413..1429
                     /label=r.wpre seq.01.56
                     /note="r.wpre seq.01.56"
                     /note="color: #ff8080; direction: RIGHT"
     primer_bind     1413..1429
                     /label=r.wpre seq.01.56
                     /note="color: black; sequence: GCAATGCCCCAACCAGT; added: 
                     2021-01-23"
     primer_bind     1576..1595
                     /label=r.wpre rev.01.3
                     /note="color: black; sequence: TTAAAGCAGCGTATCCACAT; added:
                     2021-01-23"
     primer_bind     1594..1617
                     /label=r.wpre rev.01.79
                     /note="color: black; sequence: ATAGCGTAAAAGGAGCAACATAGT; 
                     added: 2021-01-23"
     primer_bind     complement(1641..1666)
                     /label=f.wpre seq for.01.4
                     /note="color: black; sequence: AATCAACCTCTGGATTACAAAATTTG; 
                     added: 2021-01-23"
     primer_bind     1760..1783
                     /label=r.syn flex seq.01.51
                     /note="color: black; sequence: GAAGTTATGGGTCGATGGTGAGAT; 
                     added: 2021-01-23"
     misc_feature    1838..1871
                     /label=loxp2272
                     /note="loxp2272"
                     /note="color: #ff8000"
     sig_peptide     1896..1958
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /note="color: #993366
                     Cleavage site after base 1955"
     misc_feature    1896..1958
                     /label=Ig K-Chain
                     /note="Ig K-Chain"
                     /note="color: #ff8000"
     CDS             1935..1958
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /note="color: #993366
                     Cleavage site after base 1955"
                     /translation="WVPGSTGD"
     CDS             1959..3521
                     /codon_start=1
                     /note="color: #993366"
                     /translation="GSAAGSTLDKIAKNGVIVVGHRDSSVPFSYYDSQHKVVGYSQEYS
                     NAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTYDFECGSTTNNVERQKQAAFSDT
                     IFVVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAKDHG
                     DSFRTLESGRAVAFMMDDSLLAGERAKAKKPDDWEIVGKPQSQEAYGCMLRKDDPQFKK
                     LMDDTIAQVQTSGEAEKWFDKWFKNPILVNHTVYITADKQKNGIKANFKIHHNVEDGSV
                     QLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLEFVTATGNSLGMDE
                     LYKGGTGGSMSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTT
                     GKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTR
                     AEVKLEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNNPLNMNFELSDEMKALFKEPND
                     KALK"
     CDS             1959..2711
                     /codon_start=1
                     /label=ybeJ1-253
                     /note="ybeJ1-253"
                     /note="color: #8000ff"
                     /translation="GSAAGSTLDKIAKNGVIVVGHRDSSVPFSYYDSQHKVVGYSQEYS
                     NAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTYDFECGSTTNNVERQKQAAFSDT
                     IFVVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAKDHG
                     DSFRTLESGRAVAFMMDDSLLAGERAKAKKPDDWEIVGKPQSQEAYGCMLRKDDPQFKK
                     LMDDTIAQVQTSGEAEKWFDKWFKNPILV"
     primer_bind     1984..2004
                     /label=f.GluSnFr seq.33.1934
                     /note="color: black; sequence: ACAAAATTGCTAAAAATGGTG; 
                     added: 2021-01-23"
     misc_feature    2085..2087
                     /label=A43E
                     /note="color: #00ff00"
     primer_bind     2318..2339
                     /label=f.GluSnFr seq.33.1935
                     /note="color: black; sequence: TAAAGACTTTGCCAATCTGAAG; 
                     added: 2021-01-23"
     primer_bind     2614..2634
                     /label=f.GluSnFr seq.33.1936
                     /note="color: black; sequence: AATTTAAGAAGCTGATGGATG; 
                     added: 2021-01-23"
     misc_feature    2658..3431
                     /label=synthsized gene
                     /note="color: #00ffff"
     misc_feature    2769..2771
                     /label=G271K
                     /note="color: #00ff00"
     misc_feature    2900..2905
                     /label=BamHI
                     /note="color: #ff00ff"
     primer_bind     2910..2928
                     /label=f.GluSnFr seq.33.1937
                     /note="color: black; sequence: GAAAAAAGGGACCATATGG; added: 
                     2021-01-23"
     misc_feature    2934..2939
                     /label=XhoI
                     /note="color: #ff0000"
     misc_feature    2988..3005
                     /label=cpsfGFP
                     /note="cpsfGFP"
                     /note="color: #ffff00"
     misc_feature    3006..3443
                     /label=cpSF Venus
                     /note="cpSF Venus"
                     /note="color: #ffff00"
     misc_feature    3141..3143
                     /label=L395F
                     /note="color: #00ff00"
     misc_recomb     3198..3200
                     /label=G414S
                     /note="color: #00ff00"
     primer_bind     3220..3239
                     /label=f.GluSnFr seq.33.1938
                     /note="color: black; sequence: CAAGGTATCCAGACCATATG; added:
                     2021-01-23"
     misc_feature    3345..3350
                     /label=XhoI
                     /note="color: #ff0000"
     misc_feature    3426..3431
                     /label=XhoI
                     /note="color: #00ff00"
     misc_feature    3444..3521
                     /label=YbeJ254-280
                     /note="YbeJ254-280"
                     /note="color: #8000ff"
     primer_bind     3522..3544
                     /label=Vector.FOR
                     /note="color: black; sequence: ctgcaggtcgacgaacaaaaact; 
                     added: 2019-10-29"
     CDS             3534..3563
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /note="color: #cc99b2"
                     /translation="EQKLISEEDL"
     primer_bind     complement(3538..3563)
                     /label=Primer 1
                     /note="color: black; sequence: cagatcctcttctgagatgagttttt; 
                     added: 2019-11-11"
     CDS             3564..3680
                     /codon_start=1
                     /label=B16 GPI_COBL9 Anchor
                     /note="color: #33cccc"
                     /translation="SDNDYGKDGLAGASGGRRNGAITVLSFITFYVAAFMVLL"
     misc_feature    3603..3608
                     /label=BspEI
                     /note="color: #ff0000"
     misc_feature    complement(3690..3723)
                     /label=loxp
                     /note="loxp"
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     3749..3765
                     /label=r.Syn flex rev 1.01.32
                     /note="color: black; sequence: CGGGTCGATGGTGAAGC; added: 
                     2021-01-23"
     misc_feature    complement(3786..3819)
                     /label=loxp2272
                     /note="loxp2272"
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    complement(3835..3974)
                     /label=Intron
                     /note="Intron"
                     /note="color: #cc99ff; direction: LEFT"
     primer_bind     complement(3846..3869)
                     /label=f.AAV syn flex INTRON seq for.01.26
                     /note="color: black; sequence: CTGACATCCACTTTGCCTTTCTCT; 
                     added: 2021-01-23"
     primer_bind     complement(3903..3922)
                     /label=f.INTRON seq.01.77
                     /note="color: black; sequence: CTTGTCGAGACAGAGAAGAC; added:
                     2021-01-23"
     primer_bind     3903..3921
                     /label=r.INTRON seq.01.78
                     /note="color: black; sequence: GTCTTCTCTGTCTCGACAA; added: 
                     2021-01-23"
     misc_feature    complement(3975..4417)
                     /label=synapsin promoter
                     /note="synapsin promoter"
                     /note="color: #cc99ff; direction: LEFT"
     primer_bind     complement(4085..4105)
                     /label=f.hsyn seq for.01.7
                     /note="color: black; sequence: CGGTGGGCAGCGGAGGAGTCG; 
                     added: 2021-01-23"
     primer_bind     4085..4105
                     /label=r.syn seq.01.52
                     /note="color: black; sequence: CGACTCCTCCGCTGCCCACCG; 
                     added: 2021-01-23"
     primer_bind     4134..4150
                     /label=r.syn rev 2.01.45
                     /note="color: black; sequence: GCCGCAGCGCAGATGGT; added: 
                     2021-01-23"
     primer_bind     complement(4213..4229)
                     /label=f.synapsin_321_seq_for.01.33
                     /note="color: black; sequence: CACCTTGGTCGCGTCCG; added: 
                     2021-01-23"
     primer_bind     4213..4229
                     /label=r.syn seq.01.53
                     /note="color: black; sequence: CGGACGCGACCAAGGTG; added: 
                     2021-01-23"
     primer_bind     4313..4333
                     /label=r.ITR rev 2.01.18
                     /note="color: black; sequence: GCGGGGGCGAAGGCACTGTCC; 
                     added: 2021-01-23"
     primer_bind     complement(4376..4394)
                     /label=f.syn seq 1.01.44
                     /note="color: black; sequence: GAGAGGGGGAGGGGAAACA; added: 
                     2021-01-23"
     primer_bind     4418..4440
                     /label=r.ITR rev 1.01.17
                     /note="color: black; sequence: GAATGGGGGTTGGGTGCTTGTCC; 
                     added: 2021-01-23"
     primer_bind     4425..4444
                     /label=r.hsyn rev.01.25
                     /note="color: black; sequence: GGTTGGGTGCTTGTCCAGTG; added:
                     2021-01-23"
     primer_bind     complement(4426..4444)
                     /label=g.synapsin_for.01.48
                     /note="color: black; sequence: CACTGGACAAGCACCCAAC; added: 
                     2021-01-23"
     misc_feature    4533..4697
                     /label=ITR
                     /note="ITR"
                     /note="color: #ffff00"
     misc_feature    complement(4868..4891)
                     /label=r.ITR rev 4.01.20
                     /note="r.ITR rev 4.01.20"
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     complement(4868..4891)
                     /label=r.ITR rev 4.01.20
                     /note="color: black; sequence: TGGCGAGAAAGGAAGGGAAGAAAG; 
                     added: 2021-01-23"
     misc_feature    complement(4915..4933)
                     /label=r.ITR rev 3.01.19
                     /note="r.ITR rev 3.01.19"
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     complement(4915..4933)
                     /label=r.ITR rev 3.01.19
                     /note="color: black; sequence: GGAGCCCCCGATTTAGAGC; added: 
                     2021-01-23"
     misc_feature    complement(5368..5385)
                     /label=f.AAV backbone seq.01.65
                     /note="f.AAV backbone seq.01.65"
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     complement(5368..5385)
                     /label=f.AAV backbone seq.01.65
                     /note="color: black; sequence: GGGCGACACGGAAATGTT; added: 
                     2021-01-23"
     misc_feature    complement(6031..6051)
                     /label=f.AAV backbone seq.01.64
                     /note="f.AAV backbone seq.01.64"
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     complement(6031..6051)
                     /label=f.AAV backbone seq.01.64
                     /note="color: black; sequence: TATCAGCAATAAACCAGCCAG; 
                     added: 2021-01-23"
     misc_feature    complement(6342..6358)
                     /label=f.AAV backbone seq.01.63
                     /note="f.AAV backbone seq.01.63"
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     complement(6342..6358)
                     /label=f.AAV backbone seq.01.63
                     /note="color: black; sequence: GACGCTCAGTGGAACGA; added: 
                     2021-01-23"
ORIGIN
        1 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
       61 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      421 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      481 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      541 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgcgcgctcg
      601 ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc
      661 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctt
      721 gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta ggaagatcct
      781 tatcgatttt accacatttg tagaggtttt acttgcttta aaaaacctcc cacatctccc
      841 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      901 taatggttac aaataaagca atagcatcac aaattcacaa ataaagcatt tttttcactg
      961 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg ctcgaagcgg
     1021 ccggccgccc gggcgaccta gatgcattcg acggccgccc gggtcgacgg tatcgatgcg
     1081 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     1141 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     1201 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccaaggaa
     1261 aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     1321 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     1381 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     1441 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     1501 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     1561 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     1621 gaataccagt caatctttca caaattttgt aatccagagg ttgattctta agagcttcga
     1681 gtggatcatc tgcaaattcg cccttttcag tgtttcgaac ctcttcgagg gacataactt
     1741 cgtataatgt atgctatacg aagttatggg tcgatggtga gatctggact agagggtcga
     1801 tggtgatgct tggatcaaaa taggaagaat cccaatgata acttcgtata aagtatccta
     1861 tacgaagtta tactagtcat atgggatccg ccaccatgga gacagacaca ctcctgctat
     1921 gggtactgct gctctgggtt ccaggttcca ctggtgacgg aagcgctgca ggatcaacac
     1981 tggacaaaat tgctaaaaat ggtgtcatag tagttggaca cagagactct tccgtaccat
     2041 tttcttatta cgatagtcag cataaggtgg tcggatattc acaagaatac agcaatgcca
     2101 tcgtggaagc tgtgaagaag aaactgaaca aaccagatct tcaagttaag ttgattccaa
     2161 ttacttccca gaatcggata cccctcctgc aaaacgggac ttatgatttc gagtgcggta
     2221 gtactacaaa caacgtcgaa cgacaaaagc aagcagcttt ttctgacact atctttgttg
     2281 tgggaaccag actgctcacc aagaagggag gggatattaa agactttgcc aatctgaagg
     2341 ataaggcagt ggtggtgact agtggaacaa cttcagaggt tttgctgaac aaactcaatg
     2401 aggaacaaaa aatgaacatg cgcattatta gcgcaaaaga tcatggcgat tccttcagaa
     2461 cattggaaag tgggcgcgct gttgccttca tgatggatga cagcctgctc gccggagaac
     2521 gcgccaaggc aaagaaacca gatgattggg agatcgtcgg caagccacaa agccaggagg
     2581 cttatgggtg catgctcagg aaagatgacc cccaatttaa gaagctgatg gatgacacta
     2641 tagcacaagt ccaaacctcc ggagaagctg agaagtggtt cgacaaatgg tttaaaaacc
     2701 ccatcctggt caaccatacc gtctatatca cagccgataa gcagaaaaat ggaattaaag
     2761 ccaattttaa gatccaccat aatgtagaag atgggagcgt gcagttggca gaccactacc
     2821 agcaaaatac acctatcggg gatggtccag tgctgctgcc tgacaaccac tatctctcat
     2881 atcaatctgt gttgtcaaaa gatccaaatg aaaaaaggga ccatatggtg ctgctcgaat
     2941 ttgtaacagc cacagggaac tcactgggca tggacgagct ctacaaggga gggactggcg
     3001 gctcaatgtc caaaggggaa gaattgttta ccggagtcgt gcctatcctt gtcgaactgg
     3061 atggagatgt taatgggcac aaattctccg tacgcggcga gggcgaaggg gacgccacta
     3121 atgggaagct gactcttaaa tttatctgca caactggtaa attgccagtg ccctggccta
     3181 cccttgtcac caccttgagc tacggcgtcc agtgtttcgc aaggtatcca gaccatatga
     3241 agcaacatga cttttttaaa tccgctatgc ccgagggtta cgtacaagag agaaccatca
     3301 gtttcaaaga tgatggtact tacaaaactc gcgcagaagt caagctcgaa ggagacaccc
     3361 ttgtaaatcg aatagagttg aagggcattg attttaagga agacggtaat atcctcggcc
     3421 acaaactcga gtataatttt aacaacccct tgaatatgaa ctttgaactc tcagatgaaa
     3481 tgaaagccct gtttaaagag ccaaacgaca aagcacttaa actgcaggtc gacgaacaaa
     3541 aactcatctc agaagaggat ctgtcagata acgactacgg taaagatggg ttggctgggg
     3601 cttcaggagg cagacgaaat ggcgctataa ctgtgctcag tttcataacg ttctacgtgg
     3661 cagcatttat ggtattgttg tagaagctta taacttcgta tagcatacat tatacgaagt
     3721 tatgtgtcga tggtgatgct tggcaattcg ggtcgatggt gaagcattgg tcttcctatt
     3781 ttgatataac ttcgtatagg atactttata cgaagttatg gggggcgcgc catcatcacc
     3841 tgtggagaga aaggcaaagt ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa
     3901 gagtcttctc tgtctcgaca agcccagttt ctattggtct ccttaaacct gtcttgtaac
     3961 cttgatactt acctgatcca acggctgcga cttggggcag ggggtcctag gggtggtctg
     4021 gccaggagcc gcgggggcgg actgcgcggt gcccaggagc acagctgcgc tctcaggcac
     4081 gacacgactc ctccgctgcc caccgcagac tgaggcagcg ctgagtcgcc ggcgccgcag
     4141 cgcagatggt cgcgcccgtg cccccctatc tcgcgcctcg cgtggtgcgg tccggctggg
     4201 ccggcggcgg cgcggacgcg accaaggtgg ccgggaaggg gagtttgcgg gggaccggcg
     4261 agtgacgtca gcgcgccttc agtgctgagg cggcggtggc gcgcgccgcc aggcgggggc
     4321 gaaggcactg tccgcggtgc tgaagctggc agtgcgcacg cgcctcgccg catcctgttt
     4381 cccctccccc tctctgatag gggatgcgca atttggggaa tgggggttgg gtgcttgtcc
     4441 agtgggtcgg ggtcggtcgt caagcatgct ttaaatactg aaaagggcca attccagcac
     4501 actggagctc ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc
     4561 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4621 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     4681 gtgagcgagc gagcgcgcag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4741 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4801 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4861 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4921 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4981 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5041 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5101 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5161 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5221 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5281 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5341 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5401 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5461 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5521 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5581 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5641 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5701 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5761 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5821 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5881 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5941 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6001 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6061 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6121 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6181 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6241 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6301 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6361 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6421 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6481 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6541 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6601 ctctgctaat cctgttacca gtgg
//
