LOCUS       Exported                6895 bp ds-DNA     circular SYN 13-AUG-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV hSyn FLEX iGluSnFR3 v857.SGZ
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 13, 2021 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      69..524
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(156..175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     366..387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     promoter        550..654
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             655..1515
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  655 ..  723 / #ccffcc / signal sequence
                        2:  724 .. 1515 / #ccffcc
                     Cleavage site after base 723"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(873..892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     rep_origin      1686..2274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     2175..2194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     2428..2445
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     repeat_region   2511..2640
                     /label=AAV2 ITR
                     /note="color: #ffcc99"
     primer_bind     complement(2522..2533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     2583..2594
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     polyA_signal    2802..2923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(2839..2858)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     2893..2912
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     misc_feature    3001..3589
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             3107..3118
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3118"
                     /translation="IEGR"
     primer_bind     3516..3536
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     primer_bind     complement(3586..3610)
                     /label=298-F-OpenVec23in4
                     /note="color: black; sequence: 
                     taggcggccgctcgagatTatcgataatc; added: 2019-11-17"
     protein_bind    complement(3661..3694)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(3765..3798)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #31849b; direction: LEFT"
     regulatory      3823..3832
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="This feature has 2 segments:
                        1: 3823 .. 3828 / #a6acb3
                        2: 3829 .. 3832 / #a6acb3"
     misc_feature    3829..3891
                     /label=Ig K-Chain
                     /note="Ig K-Chain"
                     /note="color: #ff8000"
     CDS             3829..3891
                     /codon_start=1
                     /label=Feature 29
                     /note="color: #993366"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     sig_peptide     3829..3891
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /note="color: #993366
                     Cleavage site after base 3888"
     CDS             3868..3891
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /note="color: #993366
                     Cleavage site after base 3888"
                     /translation="WVPGSTGD"
     primer_bind     complement(3871..3891)
                     /label=Vector.REV
                     /note="color: black; sequence: GTCACCAGTGGAACCTGGAAC; 
                     added: 2019-10-29"
     CDS             3892..5454
                     /codon_start=1
                     /note="color: #993366"
                     /translation="GSAAGSTLDKIAKNGVIVVGHRDSSVPFSYYDSQHKVVGYSQEYS
                     NAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTYDFECGSTTNNVERQKQAAFSDT
                     IFVVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAKDHG
                     DSFRTLESGRAVAFMMDDSLLAGERAKAKKPDDWEIVGKPQSQEAYGCMLRKDDPQFKK
                     LMDDTIAQVQTSGEAEKWFDKWFKNPILVNHTVYITADKQKNGIKANFKIHHNVEDGSV
                     QLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLEFVTATGNSLGMDE
                     LYKGGTGGSMSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTT
                     GKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTR
                     AEVKLEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNNPLNMNFELSDEMKALFKEPND
                     KALK"
     CDS             3892..4644
                     /codon_start=1
                     /label=ybeJ1-253
                     /note="ybeJ1-253"
                     /note="color: #8000ff"
                     /translation="GSAAGSTLDKIAKNGVIVVGHRDSSVPFSYYDSQHKVVGYSQEYS
                     NAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTYDFECGSTTNNVERQKQAAFSDT
                     IFVVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAKDHG
                     DSFRTLESGRAVAFMMDDSLLAGERAKAKKPDDWEIVGKPQSQEAYGCMLRKDDPQFKK
                     LMDDTIAQVQTSGEAEKWFDKWFKNPILV"
     primer_bind     complement(4875..4895)
                     /label=Primer 4a
                     /note="color: black; sequence: CGAGAGAGTTCCCGGTGGCGGTCACG; 
                     added: 2019-12-22"
     misc_feature    4921..4938
                     /label=cpsfGFP
                     /note="cpsfGFP"
                     /note="color: #ffff00"
     misc_feature    4939..5376
                     /label=cpSF Venus
                     /note="cpSF Venus"
                     /note="color: #ffff00"
     misc_feature    5377..5454
                     /label=YbeJ254-280
                     /note="YbeJ254-280"
                     /note="color: #8000ff"
     primer_bind     5455..5477
                     /label=Vector.FOR
                     /note="color: black; sequence: ctgcaggtcgacgaacaaaaact; 
                     added: 2019-10-29"
     primer_bind     complement(5466..5496)
                     /label=299-R-OpenVec23in4
                     /note="color: black; sequence: 
                     cagatcctcttctgagatgagtttttgttcg; added: 2019-11-17"
     CDS             5467..5496
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /note="color: #cc99b2"
                     /translation="EQKLISEEDL"
     primer_bind     complement(5471..5496)
                     /label=Primer 1
                     /note="color: black; sequence: cagatcctcttctgagatgagttttt; 
                     added: 2019-11-11"
     CDS             5497..5849
                     /codon_start=1
                     /label=gBlock replacing part
                     /note="color: #ffff00"
                     /translation="NAVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLW
                     QKKPRDRHKQLRATARATDYLQADAITRIPDYRYRYQRRDRDDDRDTEPDHDRDADPVG
                     VKGFNTLPDTEID"
     CDS             5497..5559
                     /codon_start=1
                     /label=PDGFR-beta hinge
                     /note="color: #33cccc"
                     /translation="NAVGQDTQEVIVVPHSLPFKV"
     CDS             5560..5622
                     /codon_start=1
                     /label=PDGFR-beta TM domain
                     /note="color: #993366"
                     /translation="VVISAILALVVLTIISLIILI"
     CDS             5624..5647
                     /codon_start=1
                     /label=gBlock replacing part
                     /note="color: #ffff00"
                     /translation="LWQKKPR"
     CDS             5648..5847
                     /codon_start=1
                     /label=Stargazin 203-319 targeting S->D
                     /note="color: #ff6600"
                     /translation="RHKQLRATARATDYLQADAITRIPDYRYRYQRRDRDDDRDTEPDH
                     DRDADPVGVKGFNTLPDTEID"
     CDS             5848..5849
                     /codon_start=1
                     /label=PDZ domain
                     /note="color: #ff00ff"
                     /translation=""
     protein_bind    5896..5929
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    5992..6025
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(6036..6055)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
                     /note="color: black; sequence: TTGCCTTTCTCTCCACAGGT"
     intron          6038..6170
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
                     /note="color: #------"
     primer_bind     complement(6398..6408)
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     CTGGGTTCCAGGTTCCACTGGTGACGGATCCgccgcaggc; added: 
                     2019-10-29"
     repeat_region   6763..6892
                     /label=AAV2 ITR
                     /note="color: #ffcc99"
     primer_bind     complement(6809..6820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     6870..6881
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
ORIGIN
        1 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
       61 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      121 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      181 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      241 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      301 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      361 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
      421 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
      481 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
      541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1681 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1741 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1801 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1861 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1921 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1981 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2041 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2101 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2161 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2221 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2281 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2341 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2401 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2461 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgcgcgctc
     2521 gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg
     2581 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
     2641 tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct aggaagatcc
     2701 ttatcgattt taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacatctcc
     2761 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2821 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2881 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgctcgaagc
     2941 ggccgcccgg gtcgactcta gatgcatgct cgagcggccg cccgggtcga cggtatcgat
     3001 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa
     3061 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
     3121 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag
     3181 gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
     3241 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
     3301 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
     3361 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
     3421 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
     3481 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
     3541 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct
     3601 tctcgagtgg atcatctgca gaattcgccc ttttcagtgt ttcgaacctc ttcgagggac
     3661 ataacttcgt ataatgtatg ctatacgaag ttatgggtcg atggtgagat ctggactaga
     3721 gggtcgatgg tgatgcttgg atcaaaatag gaagaatccc aatgataact tcgtataaag
     3781 tatcctatac gaagttatac tagtggtacc accggtgata tcgccaccat ggagacagac
     3841 acactcctgc tatgggtact gctgctctgg gttccaggtt ccactggtga cggatccgcc
     3901 gccggaagta ctcttgataa gatcgctaaa aatggtgtaa ttgttgtcgg ccaccgagac
     3961 tcttctgtgc cattcagcta ttatgattct caacacaaag tggtgggcta ttcacaagag
     4021 tattctaacg ccattgttga agcagttaag aagaagttga ataaaccaga ccttcaagta
     4081 aaactcattc ctataacatc ccaaaaccgc atacctctct tgcaaaacgg cacttacgat
     4141 ttcgagtgtg ggagcactac taataatgtc gaacgccaga aacaagccgc attctccgac
     4201 accatcttcg tggtgggcac tcgactgttg acaaagaaag gcggtgatat taaggatttc
     4261 gcaaatctga aggataaggc agtcgtggtc accagcggga ccacctcaga agtactcctt
     4321 aataaactca atgaagaaca aaagatgaat atgcgaatca tctctgctaa ggaccacggt
     4381 gactcttttc gcacattgga aagtgggcgc gccgtagcat ttatgatgga cgactctctg
     4441 ctcgctggcg agcgcgccaa agccaaaaaa ccagacgact gggaaattgt aggtaaacct
     4501 caaagccaag aggcatatgg ctgcatgttg agaaaagatg acccccaatt caagaaactg
     4561 atggacgaca ctatagctca ggtccagact tcaggggaag ctgagaagtg gtttgataaa
     4621 tggttcaaga acccaatctt ggtaaatcat accgtttata ttaccgctga caaacaaaag
     4681 aatgggataa aggcaaattt caagattcac cataacgtag aggatggaag cgtgcagttg
     4741 gcagaccatt atcaacagaa tacccctatt ggcgacggac ccgtgctcct ccctgacaac
     4801 cactacttgt cctatcaaag tgttctctca aaggatccca atgagaagcg agaccacatg
     4861 gtacttctcg aattcgttac cgccaccggg aattcacttg gcatggatga attgtataag
     4921 gggggtactg gcggcagcat gtccaagggt gaggagcttt ttaccggagt agtaccaatt
     4981 ctcgttgaac ttgacggaga cgtcaacggg cacaagttta gcgtacgagg tgagggggag
     5041 ggtgatgcaa ccaacggtaa gcttactctc aaattcatat gcactaccgg aaagctgcca
     5101 gtaccttggc caacactggt cactaccctc tcctatggcg ttcaatgttt tgctcgatac
     5161 cctgaccaca tgaaacagca tgacttcttt aaatcagcca tgcctgaagg gtatgtccag
     5221 gagcggacta tatcatttaa agacgatgga acctataaga ctcgggctga agtgaagctc
     5281 gagggtgaca cattggttaa tcggatcgag cttaagggca tagattttaa agaagatggt
     5341 aatattcttg gccacaaatt ggagtacaat tttaataatc ctttgaacat gaactttgag
     5401 ctctccgatg aaatgaaggc actctttaaa gagccaaacg acaaagctct gaaactgcag
     5461 gtcgacgaac aaaaactcat ctcagaagag gatctgaatg ctgtgggcca ggacacgcag
     5521 gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg
     5581 gccctggtgg tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag
     5641 ccacgtgata gacacaagca gttgcgagct acagcacgcg ccaccgatta tctgcaagct
     5701 gatgcaataa ctagaatccc tgattataga tacagatatc aacgaaggga tcgcgatgat
     5761 gatcgagata cagagccaga tcatgatcgg gatgctgatc ccgtcggggt gaaaggtttc
     5821 aacactcttc cagatacaga gatagataat ccccagacga cttcagtgta ggatatcgga
     5881 tccgctagca ctagtataac ttcgtatagc atacattata cgaagttatg tgtcgatggt
     5941 gatgcttggc aattcgggtc gatggtgaag cattggtctt cctattttga tataacttcg
     6001 tataggatac tttatacgaa gttatgggga tcatcacctg tggagagaaa ggcaaagtgg
     6061 atgtcagtaa gaccaatagg tgcctatcag aaacgcaaga gtcttctctg tctcgacaag
     6121 cccagtttct attggtctcc ttaaacctgt cttgtaacct tgatacttac ctgatccaac
     6181 ggctgcgact tggggcaggg ggtcctaggg gtggtctggc caggagccgc gggggcggac
     6241 tgcgcggtgc ccaggagcac agctgcgctc tcaggcacga cacgactcct ccgctgccca
     6301 ccgcagactg aggcagcgct gagtcgccgg cgccgcagcg cagatggtcg cgcccgtgcc
     6361 cccctatctc gcgcctcgcg tggtgcggtc cggctgggcc ggcggcggcg cggacgcgac
     6421 caaggtggcc gggaagggga gtttgcgggg gaccggcgag tgacgtcagc gcgccttcag
     6481 tgctgaggcg gcggtggcgc gcgccgccag gcgggggcga aggcactgtc cgcggtgctg
     6541 aagctggcag tgcgcacgcg cctcgccgca tcctgtttcc cctccccctc tctgataggg
     6601 gatgcgcaat ttggggaatg ggggttgggt gcttgtccag tgggtcgggg tcggtcgtca
     6661 atttaaatac tgaaaagggc gaattccagc acactggagc tcggatcttc ctagagcatg
     6721 gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg
     6781 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     6841 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctg
//
