LOCUS       pAMP238_(PB-T3G-ERP2)        5954 bp    DNA     circular UNA 01-APR-2021
DEFINITION  All-in-One piggyBac transposon Destination vector for dox-inducible
            expression of Gateway cloned elements (constitutive rtTA and
            puromycin resistance).
ACCESSION   urn.local...8m-cxzvufb
VERSION     urn.local...8m-cxzvufb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        9..376
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G, modified to
                     eliminate binding sites for endogenous mammalian
                     transcription factors"
                     /locus_tag="TRE3GS promoter"
                     /ApEinfo_label="TRE3GS promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TRE3GS promoter"
     protein_bind    17..35
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /locus_tag="tet operator"
                     /ApEinfo_label="tet operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="tet operator"
     protein_bind    53..71
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /locus_tag="tet operator(1)"
                     /ApEinfo_label="tet operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="tet operator(1)"
     protein_bind    89..107
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /locus_tag="tet operator(2)"
                     /ApEinfo_label="tet operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="tet operator(2)"
     protein_bind    125..143
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /locus_tag="tet operator(3)"
                     /ApEinfo_label="tet operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="tet operator(3)"
     protein_bind    161..179
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /locus_tag="tet operator(4)"
                     /ApEinfo_label="tet operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="tet operator(4)"
     protein_bind    197..215
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /locus_tag="tet operator(5)"
                     /ApEinfo_label="tet operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="tet operator(5)"
     protein_bind    233..251
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /locus_tag="tet operator(6)"
                     /ApEinfo_label="tet operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="tet operator(6)"
     source          383..388
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /locus_tag="source:synthetic DNA construct(1)"
                     /ApEinfo_label="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="source:synthetic DNA construct(1)"
     misc_feature    383..388
                     /locus_tag="kozak"
                     /ApEinfo_label="kozak"
                     /ApEinfo_fwdcolor="#6666fe"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="kozak"
     misc_feature    389..409
                     /locus_tag="BsaI cloning site"
                     /ApEinfo_label="BsaI cloning site"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BsaI cloning site"
     source          410..418
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /locus_tag="source:synthetic DNA construct(2)"
                     /ApEinfo_label="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="source:synthetic DNA construct(2)"
     misc_feature    410..411
                     /locus_tag="stops(1)"
                     /ApEinfo_label="stops"
                     /ApEinfo_fwdcolor="#fc8008"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="stops(1)"
     misc_feature    411..418
                     /locus_tag="stops"
                     /ApEinfo_label="stops"
                     /ApEinfo_fwdcolor="#fc8008"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="stops"
     misc_feature    421..426
                     /locus_tag="BbsI"
                     /ApEinfo_label="BbsI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BbsI"
     misc_feature    435..440
                     /locus_tag="BbsI(1)"
                     /ApEinfo_label="BbsI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BbsI(1)"
     polyA_signal    623..757
                     /note="SV40 polyadenylation signal"
                     /locus_tag="SV40 poly(A) signal"
                     /ApEinfo_label="SV40 poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SV40 poly(A) signal"
     repeat_region   1033..1067
                     /note="piggyBac transposon-specific inverted terminal
                     repeat sequence (ITR)"
                     /locus_tag="piggyBac left (5') inverted repeat"
                     /ApEinfo_label="piggyBac left (5') inverted repeat"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="piggyBac left (5') inverted repeat"
     primer_bind     complement(1078..1095)
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
                     /locus_tag="M13 Forward"
                     /ApEinfo_label="M13 Forward"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13 Forward"
     primer_bind     complement(1078..1094)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13 fwd"
     primer_bind     complement(1087..1109)
                     /note="In lacZ gene"
                     /locus_tag="M13/pUC Forward"
                     /ApEinfo_label="M13/pUC Forward"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13/pUC Forward"
     primer_bind     complement(1303..1322)
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
                     /locus_tag="pRS-marker"
                     /ApEinfo_label="pRS-marker"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pRS-marker"
     misc_feature    1417..1424
                     /locus_tag="bsmbi"
                     /ApEinfo_label="bsmbi"
                     /ApEinfo_fwdcolor="#fb0106"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="bsmbi"
     primer_bind     1424..1446
                     /note="pGEX vectors, reverse primer"
                     /locus_tag="pGEX 3'"
                     /ApEinfo_label="pGEX 3'"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pGEX 3'"
     misc_feature    1473..1479
                     /locus_tag="bsmbi(1)"
                     /ApEinfo_label="bsmbi"
                     /ApEinfo_fwdcolor="#fb0106"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="bsmbi(1)"
     primer_bind     complement(1485..1503)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
                     /locus_tag="pBRforEco"
                     /ApEinfo_label="pBRforEco"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pBRforEco"
     promoter        1571..1675
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR promoter"
     CDS             1676..2536
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGAS LIKHW"
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     primer_bind     complement(1894..1913)
                     /note="Ampicillin resistance gene, reverse primer"
                     /locus_tag="Amp-R"
                     /ApEinfo_label="Amp-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Amp-R"
     misc_feature    2391..2396
                     /locus_tag="bsaI"
                     /ApEinfo_label="bsaI"
                     /ApEinfo_fwdcolor="#fb0106"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="bsaI"
     misc_feature    2392
                     /locus_tag="recoded"
                     /ApEinfo_label="recoded"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="recoded"
     misc_feature    2395
                     /locus_tag="recoded(1)"
                     /ApEinfo_label="recoded"
                     /ApEinfo_fwdcolor="#6666fe"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="recoded(1)"
     rep_origin      2707..3295
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ori"
     primer_bind     3196..3215
                     /note="pBR322 origin, forward primer"
                     /locus_tag="pBR322ori-F"
                     /ApEinfo_label="pBR322ori-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pBR322ori-F"
     primer_bind     3449..3466
                     /note="L4440 vector, forward primer"
                     /locus_tag="L4440"
                     /ApEinfo_label="L4440"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L4440"
     misc_feature    3475
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3487..3575
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     protein_bind    3672..3693
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
                     /locus_tag="CAP binding site"
                     /ApEinfo_label="CAP binding site"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="CAP binding site"
     promoter        3708..3738
                     /note="promoter for the E. coli lac operon"
                     /locus_tag="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="lac promoter"
     protein_bind    3746..3762
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="lac operator"
     primer_bind     3751..3773
                     /note="In lacZ gene"
                     /locus_tag="M13/pUC Reverse"
                     /ApEinfo_label="M13/pUC Reverse"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13/pUC Reverse"
     primer_bind     3770..3786
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13 rev"
     primer_bind     3770..3786
                     /note="In lacZ gene. Also called M13-rev"
                     /locus_tag="M13 Reverse"
                     /ApEinfo_label="M13 Reverse"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13 Reverse"
     repeat_region   3812..3874
                     /note="piggyBac transposon-specific inverted terminal
                     repeat sequence (ITR)"
                     /locus_tag="piggyBac right (3') inverted repeat"
                     /ApEinfo_label="piggyBac right (3') inverted repeat"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="piggyBac right (3') inverted repeat"
     polyA_signal    4050..4131
                     /note="SV40 polyadenylation signal"
                     /locus_tag="SV40 poly(A) signal(1)"
                     /ApEinfo_label="SV40 poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SV40 poly(A) signal(1)"
     misc_feature    <4248..4861
                     /Source="referenceToDocument: urn:local:.:cc-cr5zr92"
                     /label="PB-Tet-Puro-Gblock_Gibson_KpNI Silent
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     CDS             complement(4248..4847)
                     /created_by="wyssuser"
                     /label="Puromycin"
     misc_feature    4296
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     ligation        <4848..4861
                     /label="Ligation"
     CDS             complement(4848..4904)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /locus_tag="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="P2A"
     misc_feature    4847^4848
                     /Original_Bases="CCAT"
                     /label="CCAT"
                     /note="Geneious type: Editing History Deletion"
     CDS             complement(4914..5657)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
                     /locus_tag="Tet-On(R) 3G"
                     /ApEinfo_label="Tet-On(R) 3G"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Original_Translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLG
                     VEQPTLYWHVKNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSH
                     RDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEH
                     QVAKEERETPTTDSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPT
                     DALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG"
                     /label="Tet-On(R) 3G"
     misc_feature    5376
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     CDS             complement(5658..5681)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an
                     enterokinasecleavage site"
                     /translation="DYKDDDDK"
                     /locus_tag="FLAG"
                     /ApEinfo_label="FLAG"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="FLAG"
     regulatory      5681..5690
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong
                     initiationof translation (Kozak, 1987)"
                     /locus_tag="vertebrate consensus sequence for strong
                     initiation of translation (Kozak, 1987)"
                     /ApEinfo_label="vertebrate consensus sequence for strong
                     initiation of translation (Kozak, 1987)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="vertebrate consensus sequence for strong
                     initiation of translation (Kozak, 1987)"
     promoter        complement(5710..5921)
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
                     /locus_tag="EF-1-alpha core promoter"
                     /ApEinfo_label="EF-1-alpha core promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="EF-1-alpha core promoter"
ORIGIN      
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccgccacccg agaccattaa tgggtctcgt aatgataggc
      421 gtcttcgaat aatggaagac atactagtag accacctccc ctgcgagcta agctggacag
      481 ccaatgacgg gtaagagagt gacatttttc actaacctaa gacaggaggg ccgtcagagc
      541 tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa cctaagacag
      601 gcgcagcttc cgagggattt gagatccaga catgataaga tacattgatg agtttggaca
      661 aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc
      721 cttatttgta accattataa gctgcaataa acaagtttga tatctataac aagaaaatat
      781 atatataata agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt
      841 taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat
      901 agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg
      961 cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat
     1021 atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta agaattcact
     1081 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     1141 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     1201 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     1261 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     1321 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     1381 tctgctcccg gcatccgctt acagacaagc tgtgaccatg tctccgggag ctgcatgtgt
     1441 cagaggtttt caccgtcatc accgaaacac gcgagttcga aagggcctcg tgatacgcct
     1501 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1561 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1621 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1681 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1741 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1801 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1861 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1921 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1981 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2041 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2101 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2161 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2221 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2281 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2341 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gttcacgcgg
     2401 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2461 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2521 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2581 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2641 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2701 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2761 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2821 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2881 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2941 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3001 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3061 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3121 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3181 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3241 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3301 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3361 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3421 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggatgagcg
     3481 cccaatacgc aaaccgcctc tcaccgcgcg ttggccgatt cattaatgca gctggcacga
     3541 caggtttccc gactggaaag cgggcagtga gcgcaacgca aaccgcctct ccccgcgcgt
     3601 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3661 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3721 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     3781 tatgaccatg attacgccaa ggtcgactta accctagaaa gataatcata ttgtgacgta
     3841 cgttaaagat aatcatgcgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     3901 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     3961 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaaaca aaaactcaaa
     4021 atttcttcta taaagtaaca aaacttttag cagtgaaaaa aatgctttat ttgtgaaatt
     4081 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     4141 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     4201 taaaacctct acaaatgtgg tatggctgat tatgatcctc tggagattca tgcgccaggt
     4261 tttctggtca tgcaccacgt tcgtgggccc tcaggaacct ccacgtcagc ggtaactgtg
     4321 aagcccagtc gctcgtaaaa aggaagattc cggggagcac tggtttcgag gaacgccggc
     4381 acgccagccc gttccgcggc ctcaacacca ggaagaacga cagcgcttcc caaaccttta
     4441 ccttggtgat ctgggctgac accgacagtg gcaagaaacc acgcaggctc ctttggtcta
     4501 tgaggagcca gcaagccctc catttgctgt tgtgccgcca acctactccc tgacagctcg
     4561 gccatccgcg gacctatttc cgcgaacact gcgccggctt cgacgctttc cggagtagtc
     4621 cagaccgcaa ctgcggcacc atcgtccgca acccaaactt taccaatgtc cagacccacg
     4681 cgcgtaagaa acagctcttg caactcagtt accctctcta tgtgtctgtc cggatctacg
     4741 gtatggcggg tcgctggata atctgcaaat gctgcggcaa gtgttcggac agcccgtggt
     4801 acatcgtcac gggtagcgag cctaacagtg ggcttatatt ccgtcatggg gccggggttc
     4861 tcctccacgt cgccggcctg cttcagcagg ctgaagttgg tggcgccgct gcccccgggg
     4921 agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg
     4981 tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg
     5041 ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag gccgaaaagg
     5101 aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag aagtgggggc
     5161 atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc ctgttcctcc
     5221 aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc gttctccagg
     5281 gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta ctgtttctct
     5341 gttgggcggg tgccgagatg cactttagcc ccgtcacgat gtgagaggag agcacagcgg
     5401 tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag ggggcaggag
     5461 tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc ccgcttgttc
     5521 ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag tttccttgtc
     5581 gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat gactttgctc
     5641 ttgtccagtc tagacatctt atcgtcatcg tctttgtaat ccatggtggc ggatcccgcg
     5701 tcacgacacc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac
     5761 tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc
     5821 actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc cgacccctcc
     5881 ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc
     5941 actcgagtgg aatt
//
