LOCUS pTub-alpha1-EB3-mNeonGreen 6064 bp DNA circular 20-SEP-2017 ACCESSION DNA_pEGFP-N1 VERSION DNA_pEGFP-N1.0 SOURCE ORGANISM COMMENT Backbone pTub-alpha1-ZsGreen-N1 digested with PstI/NotI Insert: EB3-NeonGreen (PstI/NotI) from pCMV-EB3-mNeonGreen VNTEXTCHREPL|Animal/Other Eukaryotic VNTREPLTYPE|Plasmid COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|-11948736| COMMENT LSOWNER| COMMENT VNTNAME|pTub-alpha1-EB3-mNeonGreen| FEATURES Location/Qualifiers promoter 3609..3877 /promoter_type="eukaryotic" /type="eukaryotic" /vntifkey="29" /label=sv40 /note="Unknown feature type:eukaryotic" gene 3963..4751 /type="gene" /vntifkey="60" /note="Resistance gene" polyA_site 4929..5198 /type="polyA_site" /vntifkey="26" /label=TK_PA_Terminator oriT 5346..5965 /type="oriT" /vntifkey="104" /label=pB322_origen polyA_signal 2748..2973 /type="polyA_signal" /vntifkey="25" /label=SV40_PA oriT 3112..3418 /type="oriT" /vntifkey="104" /label=f1_origin promoter 6..1102 /promoter_type="eukaryotic" /type="eukaryotic" /vntifkey="29" /label=Tub-alpha-1 /note="Unknown feature type:eukaryotic" misc_structure 1103..1146 /type="misc_structure" /vntifkey="88" /label=MCS mat_peptide 1159..2001 /type="mat_peptide" /vntifkey="84" /label=EB3 /note="+TIP End Binding protein 3" misc_marker 2017..2727 /type="misc_marker" /vntifkey="22" /label=mNeonGreen /note="brigther version of GFP" BASE COUNT 1525 a 1575 c 1534 g 1430 t ORIGIN 1 tagttattat gctgaattcc gtattaggag ggatggctca tttctaggga caaaataaca 61 caggctctgg ggtgaggtag ggtcgggtaa gggggcgagc ggggaggggt gatgggagag 121 cactaccgct aaagaaagca agctctctag taaagcgtta agtactactt tgtatattgt 181 ttctcttttt tcctttctct ctctctctct ctttgtttgt ttttggtttt tgagacagga 241 tatcacacta tatatccagg ccgggtacaa aataaccgca gtcctccgct tcccaatgct 301 gggatttaca ggcataaacc actaagggcg gttatgatga ccttgagctg caagtcttcc 361 tgcctctgcc tcccgggtgc tgttgagggt cataggcgtg gtctattcat actgagcttc 421 tgaattttgc tcaaatatta ataataatag taataataat aaccacaata atacaactgt 481 aaaactaaac atttacccac gcctttttga ccatcattcc catagctctt gctactttat 541 ttaaagcgaa cagagatgtt gaatcctgac ggaacgtatt taaatttagt gtagtataaa 601 tgaaaagctg gaatttacca taaagaatct caacacaaat tctgtgatta agtgttgggg 661 aaaaccttaa attatcctaa ctacagttta aggtttaagc tccgtataat cacccaaccc 721 ccgttttctt tcttccctct ctacccctcc ccagctccac cccataatgg atgctcggct 781 agttgctttt gcgaggcctt tgtctgaagg atgcaaaatc tacggatgct agcgaggggg 841 ggaagggggg agagattacc tcataccatg tcgcttgcac caatcaccac tctcctgtcg 901 cggcttctct gggcagacgg aggggtctgg accaacagga aaaggccttg gcccatcccc 961 atggtgaccg agctgtatat aaggaggcgc atccgcccaa gtgccgcagg ttctcttaca 1021 tcgaccgctt aagagtcgcg ctgtaagaag caacacctcc tcctcgcctc cgccatccac 1081 ccggcagccg cgaagcagca acaccggtga gcgggggatc tcgagctcaa gcttcgaatt 1141 ctgcagtcga ctgccaccat ggccgtcaat gtgtactcca catctgtgac cagtgaaaat 1201 ctgagtcgcc atgatatgct tgcatgggtc aacgactccc tgcacctcaa ctataccaag 1261 atagaacagc tttgttcagg ggcagcctac tgccagttca tggacatgct cttccccggc 1321 tgtgtgcact tgaggaaagt gaagttccag gccaaactag agcatgaata catccacaac 1381 ttcaaggtgc tgcaagcagc tttcaagaag atgggtgttg acaaaatcat tcctgtagag 1441 aaattagtga aaggaaaatt ccaagataat tttgagttta ttcagtggtt taagaaattc 1501 tttgacgcaa actatgatgg aaaggattac aaccctctgc tggcgcggca gggccaggac 1561 gtagcgccac ctcctaaccc aggtgatcag atcttcaaca aatccaagaa actcattggc 1621 acagcagttc cacagaggac gtcccccaca ggcccaaaaa acatgcagac ctctggccgg 1681 ctgagcaatg tggccccccc ctgcattctc cggaagaatc ctccatcagc ccgaaatggc 1741 ggccatgaga ctgatgccca aattcttgaa ctcaaccaac agctggtgga cttgaagctg 1801 acagtggatg ggctggagaa ggaacgtgac ttctacttca gcaaacttcg tgacatcgag 1861 ctcatctgcc aggagcatga aagtgaaaac agccctgtta tctcaggcat cattggcatc 1921 ctctatgcca cagaggaagg attcgcaccc cctgaggacg atgagattga agagcatcaa 1981 caagaagacc aggacgagta cctggatccc gccaccatgg tgagcaaggg cgaggaggat 2041 aacatggcct ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg 2101 gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac 2161 ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg 2221 tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg 2281 gtagatggct ccggctacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt 2341 actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag 2401 gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcggactgg 2461 tgcaggtcga agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt 2521 tacaccactg gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc 2581 aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag 2641 ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg 2701 atgggcatgg acgagctgta caagtaatga gcggccgcga ctctagatca taatcagcca 2761 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 2821 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 2881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 2941 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt 3001 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 3061 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 3121 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 3181 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 3241 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 3301 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 3361 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 3421 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 3481 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 3541 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg 3601 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 3661 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 3721 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 3781 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 3841 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 3901 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca 3961 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 4021 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 4081 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 4141 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 4201 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 4261 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 4321 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 4381 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 4441 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 4501 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 4561 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 4621 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 4681 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 4741 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 4801 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 4861 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 4921 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 4981 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg 5041 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca 5101 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg 5161 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact 5221 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 5281 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 5341 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 5401 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 5461 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 5521 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 5581 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 5641 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 5701 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 5761 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 5821 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 5881 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 5941 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 6001 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat 6061 gcat //