LOCUS       pTub-alpha1-EB3-mNeonGreen        6064 bp    DNA     circular     20-SEP-2017
ACCESSION   DNA_pEGFP-N1
VERSION     DNA_pEGFP-N1.0
SOURCE      
  ORGANISM  
COMMENT     
            Backbone pTub-alpha1-ZsGreen-N1 digested with PstI/NotI
            
            Insert:
            EB3-NeonGreen (PstI/NotI) from pCMV-EB3-mNeonGreen
            VNTEXTCHREPL|Animal/Other Eukaryotic
            VNTREPLTYPE|Plasmid
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-11948736|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTub-alpha1-EB3-mNeonGreen|
FEATURES             Location/Qualifiers
     promoter        3609..3877
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
                     /vntifkey="29"
                     /label=sv40
                     /note="Unknown feature type:eukaryotic"
     gene            3963..4751
                     /type="gene"
                     /vntifkey="60"
                     /note="Resistance gene"
     polyA_site      4929..5198
                     /type="polyA_site"
                     /vntifkey="26"
                     /label=TK_PA_Terminator
     oriT            5346..5965
                     /type="oriT"
                     /vntifkey="104"
                     /label=pB322_origen
     polyA_signal    2748..2973
                     /type="polyA_signal"
                     /vntifkey="25"
                     /label=SV40_PA
     oriT            3112..3418
                     /type="oriT"
                     /vntifkey="104"
                     /label=f1_origin
     promoter        6..1102
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
                     /vntifkey="29"
                     /label=Tub-alpha-1
                     /note="Unknown feature type:eukaryotic"
     misc_structure  1103..1146
                     /type="misc_structure"
                     /vntifkey="88"
                     /label=MCS
     mat_peptide     1159..2001
                     /type="mat_peptide"
                     /vntifkey="84"
                     /label=EB3
                     /note="+TIP End Binding protein 3"
     misc_marker     2017..2727
                     /type="misc_marker"
                     /vntifkey="22"
                     /label=mNeonGreen
                     /note="brigther version of GFP"
BASE COUNT     1525 a      1575 c      1534 g      1430 t 
ORIGIN
        1 tagttattat gctgaattcc gtattaggag ggatggctca tttctaggga caaaataaca 
       61 caggctctgg ggtgaggtag ggtcgggtaa gggggcgagc ggggaggggt gatgggagag 
      121 cactaccgct aaagaaagca agctctctag taaagcgtta agtactactt tgtatattgt 
      181 ttctcttttt tcctttctct ctctctctct ctttgtttgt ttttggtttt tgagacagga 
      241 tatcacacta tatatccagg ccgggtacaa aataaccgca gtcctccgct tcccaatgct 
      301 gggatttaca ggcataaacc actaagggcg gttatgatga ccttgagctg caagtcttcc 
      361 tgcctctgcc tcccgggtgc tgttgagggt cataggcgtg gtctattcat actgagcttc 
      421 tgaattttgc tcaaatatta ataataatag taataataat aaccacaata atacaactgt 
      481 aaaactaaac atttacccac gcctttttga ccatcattcc catagctctt gctactttat 
      541 ttaaagcgaa cagagatgtt gaatcctgac ggaacgtatt taaatttagt gtagtataaa 
      601 tgaaaagctg gaatttacca taaagaatct caacacaaat tctgtgatta agtgttgggg 
      661 aaaaccttaa attatcctaa ctacagttta aggtttaagc tccgtataat cacccaaccc 
      721 ccgttttctt tcttccctct ctacccctcc ccagctccac cccataatgg atgctcggct 
      781 agttgctttt gcgaggcctt tgtctgaagg atgcaaaatc tacggatgct agcgaggggg 
      841 ggaagggggg agagattacc tcataccatg tcgcttgcac caatcaccac tctcctgtcg 
      901 cggcttctct gggcagacgg aggggtctgg accaacagga aaaggccttg gcccatcccc 
      961 atggtgaccg agctgtatat aaggaggcgc atccgcccaa gtgccgcagg ttctcttaca 
     1021 tcgaccgctt aagagtcgcg ctgtaagaag caacacctcc tcctcgcctc cgccatccac 
     1081 ccggcagccg cgaagcagca acaccggtga gcgggggatc tcgagctcaa gcttcgaatt 
     1141 ctgcagtcga ctgccaccat ggccgtcaat gtgtactcca catctgtgac cagtgaaaat 
     1201 ctgagtcgcc atgatatgct tgcatgggtc aacgactccc tgcacctcaa ctataccaag 
     1261 atagaacagc tttgttcagg ggcagcctac tgccagttca tggacatgct cttccccggc 
     1321 tgtgtgcact tgaggaaagt gaagttccag gccaaactag agcatgaata catccacaac 
     1381 ttcaaggtgc tgcaagcagc tttcaagaag atgggtgttg acaaaatcat tcctgtagag 
     1441 aaattagtga aaggaaaatt ccaagataat tttgagttta ttcagtggtt taagaaattc 
     1501 tttgacgcaa actatgatgg aaaggattac aaccctctgc tggcgcggca gggccaggac 
     1561 gtagcgccac ctcctaaccc aggtgatcag atcttcaaca aatccaagaa actcattggc 
     1621 acagcagttc cacagaggac gtcccccaca ggcccaaaaa acatgcagac ctctggccgg 
     1681 ctgagcaatg tggccccccc ctgcattctc cggaagaatc ctccatcagc ccgaaatggc 
     1741 ggccatgaga ctgatgccca aattcttgaa ctcaaccaac agctggtgga cttgaagctg 
     1801 acagtggatg ggctggagaa ggaacgtgac ttctacttca gcaaacttcg tgacatcgag 
     1861 ctcatctgcc aggagcatga aagtgaaaac agccctgtta tctcaggcat cattggcatc 
     1921 ctctatgcca cagaggaagg attcgcaccc cctgaggacg atgagattga agagcatcaa 
     1981 caagaagacc aggacgagta cctggatccc gccaccatgg tgagcaaggg cgaggaggat 
     2041 aacatggcct ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg 
     2101 gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac 
     2161 ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg 
     2221 tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg 
     2281 gtagatggct ccggctacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt 
     2341 actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag 
     2401 gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcggactgg 
     2461 tgcaggtcga agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt 
     2521 tacaccactg gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc 
     2581 aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag 
     2641 ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg 
     2701 atgggcatgg acgagctgta caagtaatga gcggccgcga ctctagatca taatcagcca 
     2761 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 
     2821 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 
     2881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     2941 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt 
     3001 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 
     3061 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 
     3121 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 
     3181 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 
     3241 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 
     3301 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 
     3361 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 
     3421 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
     3481 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
     3541 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg 
     3601 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 
     3661 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 
     3721 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 
     3781 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
     3841 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 
     3901 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca 
     3961 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 
     4021 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 
     4081 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 
     4141 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 
     4201 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 
     4261 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 
     4321 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 
     4381 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 
     4441 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 
     4501 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 
     4561 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 
     4621 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 
     4681 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 
     4741 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 
     4801 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 
     4861 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 
     4921 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 
     4981 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg 
     5041 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca 
     5101 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg 
     5161 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact 
     5221 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 
     5281 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 
     5341 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 
     5401 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 
     5461 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 
     5521 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 
     5581 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 
     5641 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 
     5701 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 
     5761 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 
     5821 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 
     5881 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 
     5941 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 
     6001 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat 
     6061 gcat 
//
