LOCUS       pMPS104-PB-EF1a-MG-DD-H 7478 bp ds-DNA     circular     16-AUG-2021
DEFINITION  .
ACCESSION   urn.local...2i-d5uj66i                                             
VERSION     urn.local...2i-d5uj66i                                             
FEATURES             Location/Qualifiers
     primer_bind        complement(29..51)
                        /label="pGEX 3'"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer_bind        151..170
                        /label="pRS-marker"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     CDS                complement(239..307)
                        /label="LacZ alpha"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     primer_bind        364..386
                        /label="M13/pUC Forward"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer_bind        378..395
                        /label="M13-fwd"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer_bind        378..395
                        /label="M13 Forward"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer_bind        379..395
                        /label="M13 fwd"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     repeat_region      418..480
                        /label="piggyBac right (3') inverted repeat"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       481..655
                        /label="Insulator"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer             complement(702..761)
                        /label="MPS320_Rox-Ins"
                        /note="sequence: tttaaagttaGGCGCGCCGATATCTAGgctaattacttattatcgattacaacatgactg"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       735..751
                        /label="primer 171"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             735..755
                        /label="MPS113_Rox_rev"
                        /note="sequence: CTAGATATCGGCGCGCCtaac"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     misc_feature       735..2640
                        /label="Rox-PGK-Puro-Rox"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     recombination site 752..783
                        /label="Rox"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     misc_feature       complement(831..1055)
                        /label="polyA"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             1053..1101
                        /label="MPS084_HygR_polyA_Gibson"
                        /note="sequence: CAGTCGAGGCTGATCAGCGAGCTCTAGAGCctattcctttgccctcgga"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     primer             complement(1060..1082)
                        /label="MPS082_Resistance_marker_switching_3'"
                        /note="sequence: GCTCTAGAGCTCGCTGATCAGCC"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     CDS                complement(1083..2108)
                        /label="HygR"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     CDS                complement(1083..2108)
                        /label="Translation 2108-1083"
     primer             complement(2089..2135)
                        /label="MPS083_PGK_HygR_Gibson"
                        /note="sequence: GCCTTTCGACCTGCAGCCAACGCCACCatgaaaaagcctgaactcac"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     primer             2106..2125
                        /label="MPS081_Resistance_marker_switching_5'"
                        /note="sequence: CATGGTGGCGTTGGCTGCAG"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     promoter           complement(2128..2627)
                        /label="PGK promoter"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             2641..2665
                        /label="MPS027_PGK_5'_rev"
                        /note="sequence: CGATATCAAGCTGAAGTTCCTATAC"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     primer             complement(2646..2670)
                        /label="MPS026_PGK_fwd"
                        /note="sequence: AGAAAGTATAGGAACTTCAGCTTGA"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     misc_feature       2677..2708
                        /label="Rox"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer             2704..2750
                        /label="MPS319_Rox-EF1a"
                        /note="sequence: agttattacttgtacagctcgtccatggctccggtgcccgtcagtgg"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     CDS                complement(2709..2729)
                        /label="Translation 2729-2709"
     primer             2709..2730
                        /label="MPS179_FP_3'"
                        /note="sequence: ttacttgtacagctcgtccatg"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     primer             complement(2709..2730)
                        /label="MPS178_FP_3'"
                        /note="sequence: catggacgagctgtacaagtaa"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     promoter           2731..3912
                        /label="EF-1α promoter"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     intron             2961..3903
                        /label="EF-1α intron A"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     primer_bind        3860..3880
                        /label="EF1a-F"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     primer_bind        3914..3938
                        /label="LNCX"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer             3958..3978
                        /label="mCMV_seq"
                        /note="sequence: GCTGTTTTGACCTCCATAGAA"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     primer_bind        3984..4009
                        /label="MegaGate-PolyA Cassette (1 F) - 3_PB-EF1a-TetOn3G(digested by ScaI, BsaI) Fragment 2 -AmpR_BsaI_Cleanup_gBlock (digested by SphI, SapI) Fragment2 - SapI_CleanUp_gBlock (digested by BbsI, EcoRV) Fragment2"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     protein_bind       3984..4108
                        /label="attR1"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     motif              4124..4141
                        /label="I-SceI"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     CDS                4142..4186
                        /label="Trademark"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     motif              complement(4187..4204)
                        /label="I-CeuI"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     motif              complement(4205..4213)
                        /label="I-CeuI"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             4205..4229
                        /label="MPS313_gBlock_attR2-DD_fwd"
                        /note="sequence: TTATAGTTAGAAGGAAAGCTCCATC"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     misc_feature       4205..4704
                        /label="gBlock"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     extracted region   4205..4704
                        /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer             complement(4210..4237)
                        /label="MPS314_attR2_Gibson_rev"
                        /note="sequence: GTCACTATGATGGAGCTTTCCTTCTAAC"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     attR2              complement(4229..4352)
                        /label="attR2"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     protein_bind       complement(4229..4353)
                        /label="attR2"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     CDS                4355..4675
                        /label="DD-C"
                        /ApEinfo_revcolor=#993366
                        /ApEinfo_fwdcolor=#993366
     CDS                4355..4678
                        /label="Translation 4355-4678"
     primer             4676..4704
                        /label="MPS311_BC_fwd2"
                        /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer             complement(4679..4704)
                        /label="MPS312-gBlock_attR2-DD_rev"
                        /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     primer_bind        4682..4704
                        /label="oAMP123-PCR1_NGS_BC_Fwd"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     primer_bind        4682..4704
                        /label="BC_Fwd"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     primer             4682..4704
                        /label="MPS287-barcode-fwd"
                        /note="sequence: ATACTCAGAAGATGTCACTCACC"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     motif              4710..4728
                        /label="BsaI_BC_Cloning"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     primer             complement(4734..4756)
                        /label="MPS288-barcode-rev"
                        /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     motif              4757..4781
                        /label="FP NotI_BgIII cloning"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer             complement(4787..4808)
                        /label="MPS294_barcode_rev2"
                        /note="sequence: TCTTATCATGTCTGGATCGCGG"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
     polyA_signal       4791..4925
                        /label="SV40 poly(A) signal"
                        /ApEinfo_revcolor=#ff3eee
                        /ApEinfo_fwdcolor=#ff3eee
     primer_bind        complement(4899..4925)
                        /label="MegaGate-PolyA Cassette (654 R) -3_PB-EF1a-TetOn3G (digested by ScaI, BsaI) Fragment 2 -AmpR_BsaI_Cleanup_gBlock (digested by SphI, SapI) Fragment2 - SapI_CleanUp_gBlock (digested by BbsI, EcoRV) Fragment2"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     repeat_region      5197..5231
                        /label="piggyBac left (5') inverted repeat"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer_bind        complement(5253..5273)
                        /label="M13-rev"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     primer_bind        complement(5257..5273)
                        /label="M13 Reverse"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer_bind        complement(5257..5273)
                        /label="M13 rev"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     primer_bind        complement(5270..5292)
                        /label="M13/pUC Reverse"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_binding       complement(5279..5301)
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     protein_bind       5281..5297
                        /label="lac operator"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     promoter           complement(5305..5311)
                        /label="lac promoter"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     promoter           complement(5305..5335)
                        /label="lac promoter"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     promoter           complement(5312..5329)
                        /label="lac promoter"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     promoter           complement(5330..5335)
                        /label="lac promoter"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     protein_bind       5350..5371
                        /label="CAP binding site"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     primer_bind        complement(5488..5505)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     rep_origin         complement(5641..6323)
                        /label="ColE1 origin"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
     rep_origin         complement(5659..6247)
                        /label="ori"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
     primer_bind        complement(5739..5758)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     CDS                complement(6418..7278)
                        /label="AmpR"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     primer_bind        7041..7060
                        /label="Amp-R"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     primer             7041..7060
                        /label="MPS101_Amp_rev"
                        /note="sequence: ataataccgcgccacatagc"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     promoter           complement(7279..7383)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     primer             complement(7376..7395)
                        /label="MPS112_AmpR_prom_fwd"
                        /note="sequence: cggggaaatgtgcgcggaac"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     primer_bind        7451..7469
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caagctttat tatttaaccc
      421 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
      481 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
      541 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
      601 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
      661 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtaatcgat
      721 aataagtaat tagcCTAGAT ATCGGCGCGC Ctaactttaa ataattggca ttatttaaag
      781 ttaagtactC TAGTATGTCG AGCCCCAGCT GGTTCTTTCC GCCTCAGAAG CCATAGAGCC
      841 CACCGCATCC CCAGCATGCC TGCTATTGTC TTCCCAATCC TCCCCCTTGC TGTCCTGCCC
      901 CACCCCACCC CCCAGAATAG AATGACACCT ACTCAGACAA TGCGATGCAA TTTCCTCATT
      961 TTATTAGGAA AGGACAGTGG GAGTGGCACC TTCCAGGGTC AAGGAAGGCA CGGGGGAGGG
     1021 GCAAACAACA GATGGCTGGC AACTAGAAGG CACAGTCGAG GCTGATCAGC GAGCTCTAGA
     1081 GCctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     1141 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     1201 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     1261 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     1321 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     1381 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     1441 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     1501 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     1561 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     1621 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     1681 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     1741 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     1801 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     1861 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgatGa
     1921 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     1981 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     2041 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     2101 tttttcatGG TGGCGTTGGC TGCAGGTCGA AAGGCCCGGA GATGAGGAAG AGGAGAACAG
     2161 CGCGGCAGAC GTGCGCTTTT GAAGCGTGCA GAATGCCGGG CCTCCGGAGG ACCTTCGGGC
     2221 GCCCGCCCCG CCCCTGAGCC CGCCCCTGAG CCCGCCCCCG GACCCACCCC TTCCCAGCCT
     2281 CTGAGCCCAG AAAGCGAAGG AGCAAAGCTG CTATTGGCCG CTGCCCCAAA GGCCTACCCG
     2341 CTTCCATTGC TCAGCGGTGC TGTCCATCTG CACGAGACTA GTGAGACGTG CTACTTCCAT
     2401 TTGTCACGTC CTGCACGACG CGAGCTGCGG GGCGGGGGGG AACTTCCTGA CTAGGGGAGG
     2461 AGTAGAAGGT GGCGCGAAGG GGCCACCAAA GAACGGAGCC GGTTGGCGCC TACCGGTGGA
     2521 TGTGGAATGT GTGCGAGGCC AGAGGCCACT TGTGTAGCGC CAAGTGCCCA GCGGGGCTGC
     2581 TAAAGCGCAT GCTCCAGACT GCCTTGGGAA AAGCGCCTCC CCTACCCGGT AGAATTAATT
     2641 CGATATCAAG CTGAAGTTCC TATACTTTCT catatgtaac tttaaataat tggcattatt
     2701 taaagttatt acttgtacag ctcgtccatg gctccggtgc ccgtcagtgg gcagagcgca
     2761 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2821 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2881 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2941 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     3001 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt
     3061 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     3121 gccccttcgc ctcgtgcttg agttgaggcc tggcttgggc gctggggccg ccgcgtgcga
     3181 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     3241 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     3301 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     3361 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     3421 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     3481 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     3541 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     3601 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3661 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3721 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3781 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3841 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3901 caggtgtcgt gagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     3961 gttttgacct ccatagaaga cacacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     4021 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     4081 gtaaaacaca acatatccag tcactatggc gacagaagaa gtatagggat aacagggtaa
     4141 ttgttgtaag cgcgctatga tggaggctat gccactagaa tctgcgttcg ctaccttagg
     4201 accgTTATAG TTAGAAGGAA AGCTCCATCA TAGTGACTGG ATATGTTGTG TTTTACAGTA
     4261 TTATGTAGTC TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA
     4321 CGTTTCTCGT TCAGCTTTCT TGTACAAAGT GGTAGGAGTG CAGGTGGAAa ccatctcccc
     4381 aggagaTggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat
     4441 gcttggagat ggaaagaaag ttgactcctc ccgggacaga aacaagccct ttaagtttat
     4501 gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg
     4561 tcagggagcc aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg
     4621 catcatccca ccacatgcca ctctcgtGtt cgatGTGGAG CTTCTAGAAC TGGAATGAGA
     4681 AATACTCAGA AGATGTCACT CACCatagag agaccattaa tgggtctcct aggccactga
     4741 gcaagaacta tgaatgagat ctttagacat attgcggccg ctttctccgc gatccagaca
     4801 tgataagata cattgatgag tttggacaaa ccaaaactag aatgcagtga aaaaaatgcc
     4861 ttatttgtga aatttgtgat gctattgcct tatttgtaac cattataagc tgcaataaac
     4921 aagttatcta taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa
     4981 taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt
     5041 cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc
     5101 acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg
     5161 cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta
     5221 tctttctagg gttaaaaaaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5281 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5341 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5401 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5461 tttgcgtatt gggcgcactt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5521 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5581 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5641 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5701 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5761 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5821 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5881 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5941 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6001 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6061 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6121 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6181 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6241 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6301 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6361 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6421 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6481 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6541 tgctgcaatg ataccgcgtg acccacgctc accggctcca gatttatcag caataaacca
     6601 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6661 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6721 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6781 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6841 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6901 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6961 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7021 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7081 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7141 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7201 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7261 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7321 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7381 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     7441 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//