LOCUS       Exported                4696 bp DNA     circular SYN 27-AUG-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        160..630
                     /label=Syn
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    606..1575
                     /label=sequenced
                     /note="color: #a6acb3"
     CDS             659..1405
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /note="This feature has 8 segments:
                        1:  659 .. 1276 / #993366 / TetR
                        2: 1277 .. 1282 / #cc99b2
                        3: 1283 .. 1318 / #993366 / VP16 minimal transcriptio...
                        4: 1319 .. 1321 / #cc99b2
                        5: 1322 .. 1357 / #993366 / VP16 minimal transcriptio...
                        6: 1358 .. 1360 / #cc99b2
                        7: 1361 .. 1396 / #993366 / VP16 minimal transcriptio...
                        8: 1397 .. 1405 / #cc99b2"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    1443..1919
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   1959..2099
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      2174..2629
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        2911..3015
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             3016..3876
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3016 .. 3084 / #ccffcc / signal sequence
                        2: 3085 .. 3876 / #ccffcc
                     Cleavage site after base 3084"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4047..4635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgattaac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gccaccatgg gatccgattg aattcaccat
      661 gtctagactg gacaagagca aagtcataaa ctctgctctg gaattactca atgaagtcgg
      721 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
      781 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctggcaatcg agatgctgga
      841 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
      901 caacgccaag tcattccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
      961 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     1021 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     1081 actgggctgc gtattggagg atcaggagca tcaagtagca aaagaggaaa gagagacacc
     1141 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accatcaggg
     1201 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     1261 aaagtgcgaa agcggcgggc cggccgacgc ccttgacgat tttgacttag acatgctccc
     1321 agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt
     1381 tgaccttgac atgctccccg ggtaacaagc ttgcctcgag cagcgctgct cgagagatct
     1441 acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     1501 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     1561 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
     1621 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
     1681 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
     1741 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
     1801 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
     1861 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
     1921 tgattttgta ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     1981 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     2041 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     2101 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     2161 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2221 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     2281 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2341 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     2401 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2461 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     2521 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     2581 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     2641 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     2701 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     2761 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     2821 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     2881 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2941 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3001 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3061 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3121 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3181 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3241 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3301 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3361 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3421 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3481 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3541 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3601 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3661 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3721 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3781 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3841 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3901 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3961 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4021 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     4201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4621 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4681 ccttttgctc acatgt
//
