LOCUS       Exported                7134 bp ds-DNA     circular SYN 17-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    ZLock-aTAT236
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 17, 2021 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    18..20
                     /label=Kozak sequence
     misc_feature    21..47
                     /label=FLAG
     misc_feature    60..764
                     /label=mCherry
     misc_feature    777..950
                     /label=ZA127
     misc_feature    987..1691
                     /label=aa[2-236]
     misc_feature    1698..1715
                     /label=L3
     misc_feature    1704..1715
                     /label=L4
     misc_feature    1716..2144
                     /label=LOV2WT
ORIGIN
        1 acaatcaaag gagatatacc atggactata aggacgatga tgacaaagga tctggatctg
       61 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      121 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      181 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
      241 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
      301 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      361 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
      421 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
      481 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
      541 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
      601 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
      661 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
      721 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggttct ggatccgtgg
      781 ataacaaatt caataaagaa aagacgcgtg ccggtgcgga aatccattct ctgccaaacc
      841 tgaatgttga gcagaagttt gccttcatcg tgagcctgtt tgatgatcca tctcagagcg
      901 ccaatctgct ggccgaagcc aaaaaactga acgatgccca ggccccaaaa ggttctggaa
      961 gtggttcggg ctcaggaagt gaattcgagt tcccgttcga tgtggatgcg ctgttcccgg
     1021 agcggatcac cgtgctggac cagcacctgc ggcctccggc ccgccgaccc ggaaccacaa
     1081 cgccggcccg tgtggatctg cagcagcaaa tcatgactat tgtagatgag ctgggcaagg
     1141 cttctgccaa ggcgcagcac ctccctgcac ccatcaccag cgctttgagg atgcaaagca
     1201 accggcacgt tatttacata ctgaaggaca cctcagcccg accggcaggg aaaggagcca
     1261 ttattggttt cctcaaagtt ggatacaaga agctctttgt actggatgac cgggaggctc
     1321 acaatgaggt agaacccctt tgcattctgg acttttacat ccacgagtcg gtgcaacggc
     1381 atggccacgg gcgagaactt tttcagcata tgttacagaa agagcgagtg gagccccacc
     1441 aactggccat tgaccgacca tcgccgaagc tgctcaagtt cctgaataag cactacaacc
     1501 tggagaccac agtcccacag gtgaacaact ttgtcatctt tgaaggcttc tttgcccatc
     1561 agcaccggcc ccccacttcc tctctgagag caactcgaca ctctcgtgct gctgtggccg
     1621 atcccatacc tgctgctcca gcacggaagc tgccaccaaa aagagcagag ggagacatta
     1681 agccatactc tggtaccggc tcaggaagtg gtagcttggc tactacactt gaacgtattg
     1741 agaagaactt tgtcattact gacccaagat tgccagataa tcccattata ttcgcgtccg
     1801 atagtttctt gcagttgaca gaatatagcc gtgaagaaat tttgggaaga aactgcaggt
     1861 ttctacaagg tcctgaaact gatcgcgcga cagtgagaaa aattagagat gccatagata
     1921 accaaacaga ggtcactgtt cagctgatta attatacaaa gagtggtaaa aagttctgga
     1981 acctctttca cttgcagcct atgcgagatc agaagggaga tgtccagtac tttattgggg
     2041 ttcagttgga tggaactgag catgtccgag atgctgccga gagagaggga gtcatgctga
     2101 ttaagaaaac tgcagaaaat attgatgagg cggcaaaaga actttaaaag cttgcggccg
     2161 cacagctgta tacacgtgca agccagccag aactcgcccc ggaagacccc gaggatctcg
     2221 agcaccacca tcaccatcac catcactaag tgattaacct caggtgcagg ctgcctatca
     2281 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atcgatcttt
     2341 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     2401 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     2461 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     2521 tggcaacata tgcccatatg taactagcat aaccccttgg ggcctctaaa cgggtcttga
     2581 ggggtttttt gctgaaagca tgcggaggaa attctccttg aagtttccct ggtgttcaaa
     2641 gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta aaacacgata
     2701 cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt tacagacaat
     2761 tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta tgttagagcg
     2821 aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc ctcaatagat
     2881 ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc gatggctgga
     2941 ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag cagcaatcta
     3001 gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc gttcaaaata
     3061 ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact cgacgtaaac
     3121 acgttaaata gagcttggac atatttaaca tcgggcgtgt tagctttatt aggccgatta
     3181 tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt tgccatagcc
     3241 acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt agttgagctt
     3301 tttggaattg cgatcgcata acttcgtata gcatacatta tacgaagtta taagctcgga
     3361 acgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3421 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3481 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgccccctg
     3541 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3601 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3661 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     3721 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3781 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3841 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3901 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3961 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4021 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4081 ttacgcgcag aaaaaaagga tctcaagaag atcctttgtt accaatgctt aatcagtgag
     4141 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4201 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4261 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4321 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4381 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4441 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4501 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4561 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4621 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4681 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4741 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4801 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4861 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4921 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4981 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gtccgcgcgt
     5041 ttcctgcatc ttttaatcaa atcccaagat gtgtataaac gcgccggtat gtacaggaag
     5101 aggtttatac taaactgtta cattgcaaac gtggtttcgt gtgccaagtg tgaaaaccga
     5161 tgtttaatca aggctctgac gcatttctac aaccacgact ccaagtgtgt gggtgaagtc
     5221 atgcatcttt taatcaaatc ccaagatgtg tataaaccac caaactgcca aaaaatgaaa
     5281 actgtcgaca agctctgtcc gtttgctggc aactgcaagg gtctcaatcc tatttgtaat
     5341 tattgaataa taaaacaatt ataaatgtca aatttgtttt ttattaacga tacaaaccaa
     5401 acgcaacaag aacatttgta gtattatcta taattgaaaa cgcgtagtta taatcgctga
     5461 ggtaatattt aaaatcattt tcaaatgatt cacagttaat ttgcgacaat ataattttat
     5521 tttcacataa actagacgcc ttgtcgtctt cttcttcgta ttccttctct ttttcatttt
     5581 tctcttcata aaaattaaca tagttattat cgtatccata tatgtatcta tcgtatagag
     5641 taaatttttt gttgtcataa atatatatgt cttttttaat ggggtgtata gtaccgctgc
     5701 gcatagtttt tctgtaattt acaacagtgc tattttctgg tagttcttcg gagtgtgttg
     5761 ctttaattat taaatttata taatcaatga atttgggatc gtcggttttg tacaatatgt
     5821 tgccggcata gtacgcagct tcttctagtt caattacacc attttttagc agcaccggat
     5881 taacataact ttccaaaatg ttgtacgaac cgttaaacaa aaacagttca cctccctttt
     5941 ctatactatt gtctgcgagc agttgtttgt tgttaaaaat aacagccatt gtaatgagac
     6001 gcacaaacta atatcacaaa ctggaaatgt ctatcaatat atagttgctg atggccggcc
     6061 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     6121 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     6181 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6241 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6301 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6361 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     6421 ctgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     6481 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     6541 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
     6601 tgggaggtct atataagcag acgtcgttta gtgaaccgtc agatcactag atgctttatt
     6661 gcggtagttt atcacagtta aattgctaac gccagtctcg aacttaacgt gcagaagttg
     6721 gtcgtgaggc actgggcagg taagtatcgg gccctttgtg cggggggagc ggctcggggc
     6781 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     6841 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     6901 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat
     6961 cggaccgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attccccgga
     7021 gttaatccgg gacctttaat tcaacccaac acaatatatt atagttaaat aagaattatt
     7081 atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt attt
//
