LOCUS       pExpreS2-2_verified     2864 bp ds-DNA     linear       18-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    6..113
                     /label="pKanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    114..932
                     /label="KanR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(942..1135)
                     /label="sv40 pAA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1177..1613
                     /label="pActin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1620..1757
                     /label="HSP70 core"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1758..1818
                     /label="MCS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1821..1983
                     /label="opie2.pAA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1990..2761
                     /label="ColE1/Origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2768..2858
                     /label="Actin/5c/promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 AATTCGGATC TCTGCAAGGG ATTTTGGTCA TGAACAATAA AACTGTCTGC TTACATAAAC
       61 AGTAATACAA GGGGTGTTCA TAGTATAATA CGACTCACTA TAGGAGGGCC ACCATGAGCC
      121 ACATCCAGCG CGAAACCAGC TGCAGCCGTC CGCGCCTGAA CAGCAACATG GATGCCGATC
      181 TGTACGGCTA CAAATGGGCC CGCGATAACG TGGGCCAGAG CGGCGCTACC ATCTACCGCC
      241 TGTACGGCAA ACCGGATGCC CCGGAACTGT TCCTGAAACA CGGCAAAGGC AGCGTGGCCA
      301 ACGATGTGAC CGATGAAATG GTGCGCCTGA ACTGGCTGAC CGAGTTCATG CCGCTGCCGA
      361 CCATCAAACA CTTCATCCGC ACCCCGGATG ATGCCTGGCT GCTGACCACC GCCATTCCGG
      421 GCAAAACCGC CTTCCAGGTG CTGGAAGAAT ACCCGGATAG CGGCGAAAAC ATCGTGGATG
      481 CCCTGGCCGT GTTCCTGCGC CGCCTGCACA GCATCCCGGT GTGCAACTGC CCGTTCAACA
      541 GCGATCGCGT GTTCCGCCTG GCTCAGGCCC AGAGCCGCAT GAACAACGGC CTGGTGGATG
      601 CCAGCGATTT CGATGATGAA CGCAACGGCT GGCCGGTGGA ACAGGTGTGG AAAGAGATGC
      661 ACAAACTGCT GCCGTTCAGC CCGGATTCCG TGGTGACCCA CGGCGATTTC AGCCTGGATA
      721 ACCTGATCTT CGATGAGGGC AAACTGATCG GCTGCATCGA TGTGGGCCGC GTGGGCATTG
      781 CCGATCGCTA CCAGGATCTG GCCATCCTGT GGAACTGCCT GGGCGAGTTC AGCCCGAGCC
      841 TGCAGAAACG CCTGTTCCAG AAGTACGGCA TCGATAACCC GGATATGAAC AAACTGCAGT
      901 TCCACCTGAT GCTGGATGAG TTCTTCTAAT AAGTCGACCT CGAAACTTGT TTATTGCAGC
      961 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
     1021 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTTCACGTA
     1081 ATAAGTGTGC GGCTAGCAGT CAACTAGTCC AAACTCATCA ATGTATCTTA TCATGTCTTC
     1141 ACGTAATAAG TGTGCGGCTA GCAGTCAACT ACTAGTGAAT GCCCTACTAG AAGATGTGTG
     1201 TTGCACAAAA TGTCCCTGGA ATAACCAATT TGAAGTGCAG ATAGCAGTAA ACGTAAGCTA
     1261 ATATGAATAT TATTTAACTG TAATGTTTTA ATATCGCTGG ACATTACTAA TAAACCCACT
     1321 ATAAACACAT GTACATATGT ATGTTTTGGC ATACAATGAG TAGTTGGGGA AAAAATGTGT
     1381 AAAAGCACCG TGACCATCAC AGCATAAAGA TAACCAGCTG AAGTATCGAA TATGAGTAAC
     1441 CCCCAAATTG AATCACATGC CGCAACTGAT AGGACCCATG GAAGTACACT CTTCATGGCG
     1501 ATATACAAGA CACACACAAG CACGAACACC CAGTTGCGGA GGAAATTCTC CGTAAATGAA
     1561 AACCCAATCG GCGAACAATT CATACCCATA TATGGTAAAA GTTTTGAACG CGAGCGAACT
     1621 TAAGAGCGCC GGAGTATAAA TAGAGGCGCT TCGTCTACGG AGCGACAATT CAATTCAAAC
     1681 AAGCAAAGTG AACACGTCGC TAAGCGAAAG CTAAGCAAAT AAACAAGCGC AGCTGAACAA
     1741 GCTAAACAAT CTGCAGAAGC TTGGTACCCT CGAGCTCAGC TGAATTCTGG ATCCTCTAGA
     1801 CCGGTCATAT GCGGCCGCGG ATCGATCGAT ATCTGACTAA ATCTTAGTTT GTATTGTCAT
     1861 GTTTTAATAC AATATGTTAT GTTTAAATAT GTTTTTAATA AATTTTATAA AATAATTTCA
     1921 ACTTTTATTG TAACAACATT GTCCATTTAC ACACTCCTTT CAAGCGCGTG GGACTCGATG
     1981 CTCGGCGCCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
     2041 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
     2101 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
     2161 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
     2221 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
     2281 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
     2341 GCTCCAAGCT GGGCTGTGTG CACGAACCCC Ccgttcagcc cgaccgctgc gccttatccg
     2401 gtaactatcg tcttgagtcc aacCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
     2461 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
     2521 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
     2581 TTACCTTCGG AAAAAGAGTT GGTAGGTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
     2641 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
     2701 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
     2761 TGCATGCGCT AAGCGGGCTT TATAAAACGG GCTGCGGGAC CAGTTTTCAT ATCACTACCG
     2821 TTTGAGTTCT TGTGCTGTGT GGATACTCCT CCCGACACCG AATT
//