LOCUS       pTub-alpha1-Lifeact-GFP        5292 bp    DNA     circular      5-NOV-2021
ACCESSION   DNA_pEGFP-N1
VERSION     DNA_pEGFP-N1.0
SOURCE      
  ORGANISM  
COMMENT     
            
            VNTREPLTYPE|Plasmid
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-14118624|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTub-alpha1-Lifeact-GFP|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        2837..3105
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
                     /vntifkey="29"
                     /label=sv40
                     /note="Unknown feature type:eukaryotic"
     gene            3191..3979
                     /type="gene"
                     /vntifkey="60"
                     /note="Resistance gene"
     polyA_site      4157..4426
                     /type="polyA_site"
                     /vntifkey="26"
                     /label=TK_PA_Terminator
     oriT            4574..5193
                     /type="oriT"
                     /vntifkey="104"
                     /label=pB322_origen
     polyA_signal    1976..2201
                     /type="polyA_signal"
                     /vntifkey="25"
                     /label=SV40_PA
     oriT            2340..2646
                     /type="oriT"
                     /vntifkey="104"
                     /label=f1_origin
     misc_structure  1140..1165
                     /type="misc_structure"
                     /vntifkey="88"
                     /label=MCS
     promoter        6..1102
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
                     /vntifkey="29"
                     /label=Tub-alpha-1
                     /note="Unknown feature type:eukaryotic"
     misc_marker     1166..1957
                     /type="misc_marker"
                     /vntifkey="22"
                     /label=LifeAct-GFP
     misc_marker     1238..1957
                     /type="misc_marker"
                     /vntifkey="22"
                     /label=EGFP
                     /note="EGFP"
     misc_feature    1217..1237
                     /type="misc_feature"
                     /vntifkey="21"
                     /label=Linker
     misc_structure  1166..1216
                     /type="misc_structure"
                     /vntifkey="88"
                     /label=LifeAct
                     /note="LifeAct eptide sequence"
BASE COUNT     1297 a      1412 c      1366 g      1217 t 
ORIGIN
        1 tagttattat gctgaattcc gtattaggag ggatggctca tttctaggga caaaataaca 
       61 caggctctgg ggtgaggtag ggtcgggtaa gggggcgagc ggggaggggt gatgggagag 
      121 cactaccgct aaagaaagca agctctctag taaagcgtta agtactactt tgtatattgt 
      181 ttctcttttt tcctttctct ctctctctct ctttgtttgt ttttggtttt tgagacagga 
      241 tatcacacta tatatccagg ccgggtacaa aataaccgca gtcctccgct tcccaatgct 
      301 gggatttaca ggcataaacc actaagggcg gttatgatga ccttgagctg caagtcttcc 
      361 tgcctctgcc tcccgggtgc tgttgagggt cataggcgtg gtctattcat actgagcttc 
      421 tgaattttgc tcaaatatta ataataatag taataataat aaccacaata atacaactgt 
      481 aaaactaaac atttacccac gcctttttga ccatcattcc catagctctt gctactttat 
      541 ttaaagcgaa cagagatgtt gaatcctgac ggaacgtatt taaatttagt gtagtataaa 
      601 tgaaaagctg gaatttacca taaagaatct caacacaaat tctgtgatta agtgttgggg 
      661 aaaaccttaa attatcctaa ctacagttta aggtttaagc tccgtataat cacccaaccc 
      721 ccgttttctt tcttccctct ctacccctcc ccagctccac cccataatgg atgctcggct 
      781 agttgctttt gcgaggcctt tgtctgaagg atgcaaaatc tacggatgct agcgaggggg 
      841 ggaagggggg agagattacc tcataccatg tcgcttgcac caatcaccac tctcctgtcg 
      901 cggcttctct gggcagacgg aggggtctgg accaacagga aaaggccttg gcccatcccc 
      961 atggtgaccg agctgtatat aaggaggcgc atccgcccaa gtgccgcagg ttctcttaca 
     1021 tcgaccgctt aagagtcgcg ctgtaagaag caacacctcc tcctcgcctc cgccatccac 
     1081 ccggcagccg cgaagcagca acaccggtga gcgggggatc cactagcgct accggactca 
     1141 gatctcgagc tcaagcttcg aattcatggg tgtcgcagat ttgatcaaga aattcgaaag 
     1201 catctcaaag gaagaagggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct 
     1261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 
     1321 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 
     1381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 
     1441 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 
     1501 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 
     1561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 
     1621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 
     1681 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 
     1741 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 
     1801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 
     1861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 
     1921 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata 
     1981 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 
     2041 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 
     2101 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 
     2161 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt 
     2221 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata 
     2281 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 
     2341 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 
     2401 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 
     2461 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 
     2521 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 
     2581 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 
     2641 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 
     2701 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 
     2761 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg 
     2821 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 
     2881 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 
     2941 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 
     3001 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 
     3061 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 
     3121 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc 
     3181 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 
     3241 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 
     3301 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 
     3361 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 
     3421 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 
     3481 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 
     3541 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 
     3601 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 
     3661 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat 
     3721 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 
     3781 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 
     3841 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 
     3901 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 
     3961 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg 
     4021 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 
     4081 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 
     4141 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga 
     4201 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt 
     4261 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat 
     4321 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa 
     4381 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca 
     4441 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 
     4501 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 
     4561 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 
     4621 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 
     4681 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 
     4741 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 
     4801 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 
     4861 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 
     4921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 
     4981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 
     5041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 
     5101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 
     5161 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 
     5221 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 
     5281 accgccatgc at 
//
