LOCUS       pExpreS2-1-C3-PA-10H-SI 3162 bp ds-DNA     circular     23-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    12..453
                     /label="pActin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        455..592
                     /label="HSP70core"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    470..477
                     /label="TATA box"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_structure  612..746
                     /label="Intrn"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    747..753
                     /label="MCS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    768..821
                     /label="BiP Sig Peptide"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             768..1376
                     /label="Translation 768-1376"
     misc_feature    822..827
                     /label="LINEARIZE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    828..851
                     /label="C3 Cut"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    873..1046
                     /label="ProteinA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1047..1226
                     /label="ProteinA2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1251..1376
                     /label="His Strep"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1356..1376)
                     /label="HisStrep REV"
                     /note="sequence: ggccgcatatgaccggTTCACTTTTCAAACTGTGGATG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1378..1395
                     /label="MCS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     polyA_signal    1398..1560
                     /label="opie2-pAA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1567..2286
                     /label="ColE1/origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2293..2383
                     /label="Actin 5c promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(2384..2439)
                     /label="4004"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        2405..2467
                     /label="KanR promoter, pHP7"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2503..2874
                     /label="ZeoR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2939..3132)
                     /label="sv40paa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 caactactag tGAATGCCCT ACTAGAAGAT GTGTGTTGCA CAAAATGTCC CTGGAATAAC
       61 CAATTTGAAG TGCAGATAGC AGTAAACGTA AGCTAATATG AATATTATTT AACTGTAATG
      121 TTTTAATATC GCTGGACATT ACTAATAAAC CCACTATAAA CACATGTACA TATGTATGTT
      181 TTGGCATACA ATGAGTAGTT GGGGAAAAAA TGTGTAAAAG CACCGTGACC ATCACAGCAT
      241 AAAGATAACC AGCTGAAGTA TCGAATATGA GTAACCCCCA AATTGAATCA CATGCCGCAA
      301 CTGATAGGAC CCATGGAAGT ACACTCTTCA TGGCGATATA CAAGACACAC ACAAGCACGA
      361 ACACCCAGTT GCGGAGGAAA TTCTCCGTAA ATGAAAACCC AATCGGCGAA CAATTCATAC
      421 CCATATATGG TAAAAGTTTT GAACGCGAGC GAActtaaga gcgccggagt ataaatagag
      481 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
      541 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca gaagcttggt
      601 accctcgagc tcgtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      661 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      721 atccactttg cctttctctc cacaggaatt ctgGATCCGA TCTCAATATG AAGTTATGCA
      781 TATTACTGGC CGTCGTGGCC TTTGTTGGCC TCTCGCTCGG GAGATCTttg gaggttctgt
      841 ttcagggtcc aGCCGCCCAG CATGACGAGG CCGTGGATAA CAAGTTCAAT AAGGAGCAGC
      901 AGAACGCCTT CTACGAGATC CTGCACCTGC CCAACCTGAA TGAGGAGCAG CGCAATGCCT
      961 TCATTCAGAG CCTGAAGGAC GATCCATCCC AGTCGGCCAA CCTGCTGGCC GAGGCCAAGA
     1021 AGCTGAATGA CGCCCAGGCC CCGAAGGTGG ACAACAAGTT TAATAAGGAG CAGCAGAATG
     1081 CCTTTTATGA GATCCTGCAT CTGCCAAATC TGAACGAGGA GCAGCGCAAC GCCTTTATCC
     1141 AGTCCCTGAA GGATGATCCA AGCCAGTCCG CCAATTTATT AGCTGAAGCC AAGAAGCTGA
     1201 ACGATGCCCA GGCCCCCAAG GTGGATGCCA ACTCCTCGAG CGTGCCATCG CACCATCATC
     1261 ACCATCACCA TCACCACCAT GGTTCTGGTT GGAGTCACCC ACAATTCGAG AAAGGTGGTG
     1321 GCTCTGGTGG CGGTTCAGGC GGCTCCGCCT GGTCCCATCC ACAGTTTGAA AAGTGAAccg
     1381 gtcatatgcg gccgcggatc gatcgatatc tgactaaatc ttagtttgta ttgtcatgtt
     1441 ttaatacaat atgttatgtt taaatatgtt tttaataaat tttataaaat aatttcaact
     1501 tttattgtaa caacattgtc catttacaca ctcctttcaa gcgcgtggga ctcgatgctc
     1561 ggcgccACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA
     1621 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
     1681 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
     1741 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
     1801 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA
     1861 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT
     1921 CCAAGCTGGG CTGTGTGCAC GAACCCCCCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1981 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     2041 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     2101 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2161 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2221 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     2281 GATTTTgcat gcgctaagcg ggctttataa aacgggctgc gggaccagtt ttcatatcac
     2341 taccgtttga gttcttgtgc tgtgtggata ctcctcccga caccgaatta attcggatct
     2401 ctgcaaggga ttttggtcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     2461 gggtgttcat agtataatac gactcactat aggagggcca ccatggccaa gttgaccagt
     2521 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     2581 ctcgggttca gccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     2641 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     2701 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     2761 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     2821 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgaccg
     2881 acgccgacca acaccgccgg tccgacggcg gcccacgggt cccagggggg tcgacctcGA
     2941 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA
     3001 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT
     3061 TATCATGTCT TCACGTAATA AGTGTGCGGC TAGCAGTCAA CTAGTCCAAA CTCATCAATG
     3121 TATCTTATCA TGtcttcacg taataagtgt gcggctagca gt
//