LOCUS       pExpreS2-1-CR-PA        3030 bp ds-DNA     circular     23-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    17..458
                     /label="pActin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        460..597
                     /label="HSP70core"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    475..482
                     /label="TATA box"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_structure  617..751
                     /label="Intrn"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    752..758
                     /label="MCS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    773..826
                     /label="BiP Sig Peptide"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             773..1249
                     /label="Translation 773-1249"
     misc_feature    833..850
                     /label="Thrombin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(833..852)
                     /label="Reverse Overhang"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    872..1045
                     /label="ProteinA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1046..1225
                     /label="ProteinA2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1247..1249
                     /label="Stop"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1251..1268
                     /label="MCS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     polyA_signal    1271..1433
                     /label="opie2-pAA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1440..2159
                     /label="ColE1/origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2166..2256
                     /label="Actin 5c promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(2257..2312)
                     /label="4004"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        2278..2340
                     /label="KanR promoter, pHP7"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2376..2747
                     /label="ZeoR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2812..3005)
                     /label="sv40paa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gcagtcaact actagtGAAT GCCCTACTAG AAGATGTGTG TTGCACAAAA TGTCCCTGGA
       61 ATAACCAATT TGAAGTGCAG ATAGCAGTAA ACGTAAGCTA ATATGAATAT TATTTAACTG
      121 TAATGTTTTA ATATCGCTGG ACATTACTAA TAAACCCACT ATAAACACAT GTACATATGT
      181 ATGTTTTGGC ATACAATGAG TAGTTGGGGA AAAAATGTGT AAAAGCACCG TGACCATCAC
      241 AGCATAAAGA TAACCAGCTG AAGTATCGAA TATGAGTAAC CCCCAAATTG AATCACATGC
      301 CGCAACTGAT AGGACCCATG GAAGTACACT CTTCATGGCG ATATACAAGA CACACACAAG
      361 CACGAACACC CAGTTGCGGA GGAAATTCTC CGTAAATGAA AACCCAATCG GCGAACAATT
      421 CATACCCATA TATGGTAAAA GTTTTGAACG CGAGCGAAct taagagcgcc ggagtataaa
      481 tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc
      541 taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagaagc
      601 ttggtaccct cgagctcgta agtatcaagg ttacaagaca ggtttaagga gaccaataga
      661 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
      721 ctgacatcca ctttgccttt ctctccacag gaattctgGA TCCGATCTCA ATATGAAGTT
      781 ATGCATATTA CTGGCCGTCG TGGCCTTTGT TGGCCTCTCG CTCGGGAGAT CTCTGGTGCC
      841 ACGCGGATCG GCCGCCCAGC ATGACGAGGC CGTGGATAAC AAGTTCAATA AGGAGCAGCA
      901 GAACGCCTTC TACGAGATCC TGCACCTGCC CAACCTGAAT GAGGAGCAGC GCAATGCCTT
      961 CATTCAGAGC CTGAAGGACG ATCCATCCCA GTCGGCCAAC CTGCTGGCCG AGGCCAAGAA
     1021 GCTGAATGAC GCCCAGGCCC CGAAGGTGGA CAACAAGTTT AATAAGGAGC AGCAGAATGC
     1081 CTTTTATGAG ATCCTGCATC TGCCAAATCT GAACGAGGAG CAGCGCAACG CCTTTATCCA
     1141 GTCCCTGAAG GATGATCCAA GCCAGTCCGC CAATTTATTA GCTGAAGCCA AGAAGCTGAA
     1201 CGATGCCCAG GCCCCCAAGG TGGATGCCAA CTCCTCGAGC GTGCCATAAA ccggtcatat
     1261 gcggccgcgg atcgatcgat atctgactaa atcttagttt gtattgtcat gttttaatac
     1321 aatatgttat gtttaaatat gtttttaata aattttataa aataatttca acttttattg
     1381 taacaacatt gtccatttac acactccttt caagcgcgtg ggactcgatg ctcggcgccA
     1441 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
     1501 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
     1561 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1621 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1681 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1741 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1801 GGGCTGTGTG CACGAACCCC CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
     1861 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
     1921 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
     1981 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
     2041 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
     2101 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTg
     2161 catgcgctaa gcgggcttta taaaacgggc tgcgggacca gttttcatat cactaccgtt
     2221 tgagttcttg tgctgtgtgg atactcctcc cgacaccgaa ttaattcgga tctctgcaag
     2281 ggattttggt catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2341 catagtataa tacgactcac tataggaggg ccaccatggc caagttgacc agtgccgttc
     2401 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     2461 tcagccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     2521 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     2581 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     2641 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     2701 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga ccgacgccga
     2761 ccaacaccgc cggtccgacg gcggcccacg ggtcccaggg gggtcgacct cGAAACTTGT
     2821 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG
     2881 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG
     2941 TCTTCACGTA ATAAGTGTGC GGCTAGCAGT CAACTAGTCC AAACTCATCA ATGTATCTTA
     3001 TCATGtcttc acgtaataag tgtgcggcta
//