LOCUS       pExpreS2-1-C3-PA        3036 bp ds-DNA     circular     23-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    29..470
                     /label="pActin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        472..609
                     /label="HSP70core"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    487..494
                     /label="TATA box"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_structure  629..763
                     /label="Intrn"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    764..770
                     /label="MCS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    785..838
                     /label="BiP Sig Peptide"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             785..1267
                     /label="Translation 785-1267"
     misc_feature    839..844
                     /label="LINEARIZE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    845..868
                     /label="C3 Cut"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    890..1063
                     /label="ProteinA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1064..1243
                     /label="ProteinA2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1265..1267
                     /label="Stop"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1269..1286
                     /label="MCS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     polyA_signal    1289..1451
                     /label="opie2-pAA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1458..2177
                     /label="ColE1/origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2184..2274
                     /label="Actin 5c promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(2275..2330)
                     /label="4004"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        2296..2358
                     /label="KanR promoter, pHP7"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2394..2765
                     /label="ZeoR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2830..3023)
                     /label="sv40paa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 agtgtgcggc tagcagtcaa ctactagtGA ATGCCCTACT AGAAGATGTG TGTTGCACAA
       61 AATGTCCCTG GAATAACCAA TTTGAAGTGC AGATAGCAGT AAACGTAAGC TAATATGAAT
      121 ATTATTTAAC TGTAATGTTT TAATATCGCT GGACATTACT AATAAACCCA CTATAAACAC
      181 ATGTACATAT GTATGTTTTG GCATACAATG AGTAGTTGGG GAAAAAATGT GTAAAAGCAC
      241 CGTGACCATC ACAGCATAAA GATAACCAGC TGAAGTATCG AATATGAGTA ACCCCCAAAT
      301 TGAATCACAT GCCGCAACTG ATAGGACCCA TGGAAGTACA CTCTTCATGG CGATATACAA
      361 GACACACACA AGCACGAACA CCCAGTTGCG GAGGAAATTC TCCGTAAATG AAAACCCAAT
      421 CGGCGAACAA TTCATACCCA TATATGGTAA AAGTTTTGAA CGCGAGCGAA cttaagagcg
      481 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag
      541 tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca
      601 atctgcagaa gcttggtacc ctcgagctcg taagtatcaa ggttacaaga caggtttaag
      661 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac
      721 ctattggtct tactgacatc cactttgcct ttctctccac aggaattctg GATCCGATCT
      781 CAATATGAAG TTATGCATAT TACTGGCCGT CGTGGCCTTT GTTGGCCTCT CGCTCGGGAG
      841 ATCTttggag gttctgtttc agggtccaGC CGCCCAGCAT GACGAGGCCG TGGATAACAA
      901 GTTCAATAAG GAGCAGCAGA ACGCCTTCTA CGAGATCCTG CACCTGCCCA ACCTGAATGA
      961 GGAGCAGCGC AATGCCTTCA TTCAGAGCCT GAAGGACGAT CCATCCCAGT CGGCCAACCT
     1021 GCTGGCCGAG GCCAAGAAGC TGAATGACGC CCAGGCCCCG AAGGTGGACA ACAAGTTTAA
     1081 TAAGGAGCAG CAGAATGCCT TTTATGAGAT CCTGCATCTG CCAAATCTGA ACGAGGAGCA
     1141 GCGCAACGCC TTTATCCAGT CCCTGAAGGA TGATCCAAGC CAGTCCGCCA ATTTATTAGC
     1201 TGAAGCCAAG AAGCTGAACG ATGCCCAGGC CCCCAAGGTG GATGCCAACT CCTCGAGCGT
     1261 GCCATAAAcc ggtcatatgc ggccgcggat cgatcgatat ctgactaaat cttagtttgt
     1321 attgtcatgt tttaatacaa tatgttatgt ttaaatatgt ttttaataaa ttttataaaa
     1381 taatttcaac ttttattgta acaacattgt ccatttacac actcctttca agcgcgtggg
     1441 actcgatgct cggcgccACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA
     1501 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
     1561 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1621 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1681 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1741 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1801 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC CGGTAAGACA CGACTTATCG
     1861 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
     1921 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC
     1981 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
     2041 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
     2101 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2161 TCACGTTAAG GGATTTTgca tgcgctaagc gggctttata aaacgggctg cgggaccagt
     2221 tttcatatca ctaccgtttg agttcttgtg ctgtgtggat actcctcccg acaccgaatt
     2281 aattcggatc tctgcaaggg attttggtca tgaacaataa aactgtctgc ttacataaac
     2341 agtaatacaa ggggtgttca tagtataata cgactcacta taggagggcc accatggcca
     2401 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     2461 ggaccgaccg gctcgggttc agccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     2521 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     2581 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     2641 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     2701 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     2761 aggactgacc gacgccgacc aacaccgccg gtccgacggc ggcccacggg tcccaggggg
     2821 gtcgacctcG AAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
     2881 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
     2941 TCAATGTATC TTATCATGTC TTCACGTAAT AAGTGTGCGG CTAGCAGTCA ACTAGTCCAA
     3001 ACTCATCAAT GTATCTTATC ATGtcttcac gtaata
//