LOCUS       pExpreS2-1-CR-PA-10H-SI 3156 bp ds-DNA     circular     23-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    29..470
                     /label="pActin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        472..609
                     /label="HSP70core"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    487..494
                     /label="TATA box"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_structure  629..763
                     /label="Intrn"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    764..770
                     /label="MCS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    785..838
                     /label="BiP Sig Peptide"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             785..1388
                     /label="Translation 785-1388"
     misc_feature    845..862
                     /label="Thrombin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    884..1057
                     /label="ProteinA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1058..1237
                     /label="ProteinA2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1259..1278
                     /label="HisStrep FWD"
                     /note="sequence: CCAACTCCTCGAGCGTGCCATCGCACCATCATCACCATCA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1262..1387
                     /label="His Strep"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1367..1387)
                     /label="HisStrep REV"
                     /note="sequence: ggccgcatatgaccggTTCACTTTTCAAACTGTGGATG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1389..1406
                     /label="MCS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     polyA_signal    1409..1571
                     /label="opie2-pAA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1578..2297
                     /label="ColE1/origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2304..2394
                     /label="Actin 5c promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(2395..2450)
                     /label="4004"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        2416..2478
                     /label="KanR promoter, pHP7"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2514..2885
                     /label="ZeoR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2950..3143)
                     /label="sv40paa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 agtgtgcggc tagcagtcaa ctactagtGA ATGCCCTACT AGAAGATGTG TGTTGCACAA
       61 AATGTCCCTG GAATAACCAA TTTGAAGTGC AGATAGCAGT AAACGTAAGC TAATATGAAT
      121 ATTATTTAAC TGTAATGTTT TAATATCGCT GGACATTACT AATAAACCCA CTATAAACAC
      181 ATGTACATAT GTATGTTTTG GCATACAATG AGTAGTTGGG GAAAAAATGT GTAAAAGCAC
      241 CGTGACCATC ACAGCATAAA GATAACCAGC TGAAGTATCG AATATGAGTA ACCCCCAAAT
      301 TGAATCACAT GCCGCAACTG ATAGGACCCA TGGAAGTACA CTCTTCATGG CGATATACAA
      361 GACACACACA AGCACGAACA CCCAGTTGCG GAGGAAATTC TCCGTAAATG AAAACCCAAT
      421 CGGCGAACAA TTCATACCCA TATATGGTAA AAGTTTTGAA CGCGAGCGAA cttaagagcg
      481 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag
      541 tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca
      601 atctgcagaa gcttggtacc ctcgagctcg taagtatcaa ggttacaaga caggtttaag
      661 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac
      721 ctattggtct tactgacatc cactttgcct ttctctccac aggaattctg GATCCGATCT
      781 CAATATGAAG TTATGCATAT TACTGGCCGT CGTGGCCTTT GTTGGCCTCT CGCTCGGGAG
      841 ATCTCTGGTG CCACGCGGAT CGGCCGCCCA GCATGACGAG GCCGTGGATA ACAAGTTCAA
      901 TAAGGAGCAG CAGAACGCCT TCTACGAGAT CCTGCACCTG CCCAACCTGA ATGAGGAGCA
      961 GCGCAATGCC TTCATTCAGA GCCTGAAGGA CGATCCATCC CAGTCGGCCA ACCTGCTGGC
     1021 CGAGGCCAAG AAGCTGAATG ACGCCCAGGC CCCGAAGGTG GACAACAAGT TTAATAAGGA
     1081 GCAGCAGAAT GCCTTTTATG AGATCCTGCA TCTGCCAAAT CTGAACGAGG AGCAGCGCAA
     1141 CGCCTTTATC CAGTCCCTGA AGGATGATCC AAGCCAGTCC GCCAATTTAT TAGCTGAAGC
     1201 CAAGAAGCTG AACGATGCCC AGGCCCCCAA GGTGGATGCC AACTCCTCGA GCGTGCCATC
     1261 GCACCATCAT CACCATCACC ATCACCACCA TGGTTCTGGT TGGAGTCACC CACAATTCGA
     1321 GAAAGGTGGT GGCTCTGGTG GCGGTTCAGG CGGCTCCGCC TGGTCCCATC CACAGTTTGA
     1381 AAAGTGAAcc ggtcatatgc ggccgcggat cgatcgatat ctgactaaat cttagtttgt
     1441 attgtcatgt tttaatacaa tatgttatgt ttaaatatgt ttttaataaa ttttataaaa
     1501 taatttcaac ttttattgta acaacattgt ccatttacac actcctttca agcgcgtggg
     1561 actcgatgct cggcgccACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA
     1621 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
     1681 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1741 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1801 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1861 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1921 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC CGGTAAGACA CGACTTATCG
     1981 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
     2041 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC
     2101 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
     2161 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
     2221 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2281 TCACGTTAAG GGATTTTgca tgcgctaagc gggctttata aaacgggctg cgggaccagt
     2341 tttcatatca ctaccgtttg agttcttgtg ctgtgtggat actcctcccg acaccgaatt
     2401 aattcggatc tctgcaaggg attttggtca tgaacaataa aactgtctgc ttacataaac
     2461 agtaatacaa ggggtgttca tagtataata cgactcacta taggagggcc accatggcca
     2521 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     2581 ggaccgaccg gctcgggttc agccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     2641 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     2701 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     2761 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     2821 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     2881 aggactgacc gacgccgacc aacaccgccg gtccgacggc ggcccacggg tcccaggggg
     2941 gtcgacctcG AAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
     3001 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
     3061 TCAATGTATC TTATCATGTC TTCACGTAAT AAGTGTGCGG CTAGCAGTCA ACTAGTCCAA
     3121 ACTCATCAAT GTATCTTATC ATGtcttcac gtaata
//