LOCUS       GH984_pBI101_1_G        9309 bp    DNA        circular     04-AUG-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     ID   PBI101     preliminary; DNA; SYN; 5350 BP.
COMMENT     XX
COMMENT     AC   U12639; IG1034; X04996; X04995; V00087; J01541; M61152;
            M38354;
COMMENT     AC   S54410; M14641;
COMMENT     XX
COMMENT     DT   01-FEB-1993 (Rel. 7, Created)
COMMENT     DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
COMMENT     XX
COMMENT     DE   Higher plants/E.coli plasmid vector pBI101 - incomplete.
COMMENT     XX
COMMENT     KW   cloning vector.
COMMENT     XX
COMMENT     OS   Cloning vector
COMMENT     OC   Artificial sequences; Cloning vehicles.
COMMENT     XX
COMMENT     RN   [1]
COMMENT     RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
COMMENT     RC   pBI121 from pBI101.1 & CMV 35S promoter
COMMENT     RC   pBI131 from pBI101.1 & tobacco rbcS promoter
COMMENT     RA   Jefferson R.A., Kavanaugh T.A., Bevan M.W.;
COMMENT     RT   "GUS fusions: beta-glucuronidase as a sensitive and versatile
            gene
COMMENT     RT   fusion marker in higher plants";
COMMENT     RL   EMBO J. 6:3901-3907(1987).
COMMENT     XX
COMMENT     RN   [2]
COMMENT     RP   1-5350
COMMENT     RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
COMMENT     RA   Wei W., Lindsey K.;
COMMENT     RT   "T-DNA sequence of the GUS gene fusion vector pBI101";
COMMENT     RL   Unpublished (1994).
COMMENT     XX
COMMENT     RN   [3]
COMMENT     RP   1-5350
COMMENT     RC   pBI101.1
COMMENT     RA   Wei W.;
COMMENT     RT   ;
COMMENT     RL   Submitted (22-JUL-1994) by:
COMMENT     RL   Wei W., Botany, University of Leicester, University Road,
COMMENT     RL   Leicester, LE1 7RH, UK.
COMMENT     XX
COMMENT     CC   NM (pBI101)
COMMENT     CC   CM (no)
COMMENT     CC   NA (ds-DNA)
COMMENT     CC   TP (linear)
COMMENT     CC   ST ()
COMMENT     CC   TY (plasmid)
COMMENT     CC   SP (CLONTECH)
COMMENT     CC   HO (E.coli)(plant)
COMMENT     CC   CP ()
COMMENT     CC   FN (expression)(reporter)
COMMENT     CC   SE ()
COMMENT     CC   PA (pBIN19)
COMMENT     CC   BR (pBI101.2)(pBI101.3)
COMMENT     CC   OF (pBI121)(pBI221)
COMMENT     CC   OR ()
COMMENT     XX
COMMENT     FH   Key             Location/Qualifiers
COMMENT     FH
COMMENT     XX
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             386..1180
                     /note="ANT E. coli neomycin phosphotransferase II gene
                     (NPTII); neomycin resistance gene (neo) E. coli kanamycin
                     resistance gene (kan)"
                     /locus_tag="ANT E. coli neomycin phosphotransferase II
                     gene (N"
                     /label="ANT E. coli neomycin phosphotransferase II gene
                     (N"
                     /ApEinfo_label="ANT E. coli neomycin phosphotransferase II
                     gene (N"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             6511..8319
                     /note="GEN E. coli beta-glucuronidase gene (GUS)"
                     /locus_tag="GEN E. coli beta-glucuronidase gene (GUS)"
                     /label="GEN E. coli beta-glucuronidase gene (GUS)"
                     /ApEinfo_label="GEN E. coli beta-glucuronidase gene (GUS)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           8390..8642
                     /note="TER 3'UTR of the nopaline synthase gene"
                     /locus_tag="TER 3'UTR of the nopaline synthase gene"
                     /label="TER 3'UTR of the nopaline synthase gene"
                     /ApEinfo_label="TER 3'UTR of the nopaline synthase gene"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_unit     9285..9309
                     /note="the left border repeat"
                     /locus_tag="the left border repeat"
                     /label="the left border repeat"
                     /ApEinfo_label="the left border repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2516..2521
                     /locus_tag="SalI"
                     /label="SalI"
                     /ApEinfo_label="SalI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6487..6492
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2540..6486
                     /locus_tag="ProGOXL3"
                     /label="ProGOXL3"
                     /ApEinfo_label="ProGOXL3"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2522..2539
                     /locus_tag="part of 3'UTR of another gene"
                     /label="part of 3'UTR of another gene"
                     /ApEinfo_label="part of 3'UTR of another gene"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
      181 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
      241 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
      301 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
      361 ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
      421 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
      481 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
      541 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
      601 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      661 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      721 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      781 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
      841 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
      901 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
      961 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     1021 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     1081 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     1141 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     1201 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     1261 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     1321 ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
     1861 ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
     1921 tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
     1981 aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
     2041 taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
     2101 tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
     2161 ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
     2221 tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
     2281 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2341 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     2401 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     2461 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
     2521 cgagtaaaac aataataacg gcggttaaaa atagtttttt taaaaaaatg ctcaaacgtg
     2581 tgggtgggtt ctctcagttt tggtgatgct agattacttt attgggctta gaggaaaggc
     2641 ccaaacatca gaagataaaa cccaagtttg tggtttgcta gcttataggt tcatcttggt
     2701 tatgtcgctt attaaccact agttttggtg tgatgtattt aggaattatt atgcagcgtg
     2761 atagaatgtt tatgtttctg ttgaggaaag aatatgatgg taaatattac tgcgaaaata
     2821 agattgacgt tgacaacctg aaacaatgaa ttaaaaaatt ataagaatgt taacgtacgg
     2881 ttgatgcaat gagtgtaagc ttgtaaagag ttgatgcaat gaattttaac ggtcaatcat
     2941 cagattcata atatcgatgt tcatgttacg tccataaaaa actgaggtat ggccaaataa
     3001 taataagaaa attataaagc aaagaaatga gagtgaaaca tatcctgtgt gttcatagtt
     3061 tcatgaatga attatgtaat tgtcaacggt ggatcaatca ttgaatttgg tggatatcta
     3121 tatttgtaca catgtataaa cgaatgcatt ccctcaaaga tattatttgg acagattgac
     3181 aacacacata gctagattat ctatataaat catacgataa cagaataaaa atgtaattga
     3241 gaatagagta aatcaaatct gcatcatata tatacagctc atatgaatta gagaataaac
     3301 aatagaaaac aaacatcttg gtcgcttttg ttttatatac gtttcgatgt attatgtata
     3361 tgtacacatg caaacataaa ctgtccagtg cagagattct tatttcttta cagtttgtaa
     3421 tatgaggaaa aaaaaaacat atatatactt aattagagca gcttttggac atggttttag
     3481 atttatatgc aaatggtcaa atgtattaaa acaattaatt atatttttcg acgaaataat
     3541 tgatttattc tgggatatat tttggcgatc ttgatcatta taaaactttt ctttgttgct
     3601 tagaatattt atctggaagg acagttggac caacgagaca gaataaaaat cttctttcta
     3661 catcaaattt atttataaac acgggtttat agtttgaaaa agtacacttt taaacacgcc
     3721 caacgcattt gtttgtcata tttgttattc gtgtcatgta tagaacttgt ataaaccttg
     3781 tatttacgat tcactggatt tttgtggtga cggtggagta cttaaaaagt aaatatcaaa
     3841 agaaattaca cgtatgagat aatcagaaca ttgacttgct taattacaat tatggttatc
     3901 aatttatcat tataatgttt gatgttgaca aagcatcatt tcacgaacaa aatgactaaa
     3961 caatcagcag aattattcaa ccgctcgcgt ataatgttaa tattcaatca tagaaattgt
     4021 tccttcgacg acaattaaca tttaatatta gtacatttca ggtcggttcc acgtcttcat
     4081 aatgctaatt aatcctaacc gagaaaagtt ctcagtattg accgtagtta tttaacttga
     4141 gatatatcac tgtttactaa gcttaagttt taattatttg cataatcaat tcatatacag
     4201 gatttaaaat taagggttta ttagaaaagt agacactttt tcaaacataa tttacaaact
     4261 aggtagtttt tttttaaatt agagaaataa acactttttt tactttttgt gagatagagg
     4321 atttgtgtaa taaactacaa gacaaaatat atatcaaata ctaaaatgcc tttatattaa
     4381 gagataagag aaaaaatata tgggaccact tttctttaaa acatttcctc tttattttta
     4441 tttttatttt atttttttta aatatacttt taaaaataac tttttttttt aatattataa
     4501 tgacttttat atttatatat catcaatctc atgtattcgt acaccagtat tttaaacata
     4561 gttttgtaca ctaacatttg tacaccagat tatcatatac aaaaaaatta ttataatatc
     4621 tcataaatca ttaaatacat cgatttgtct catatttata aattgaagta attattgttc
     4681 ataagattaa tacaaacatg atagtaatgt aaactatagg taatactaaa tatgattgtg
     4741 tacaccatca tgtacaccat taaattgaaa tatgatgata cataccatta aatttgaagt
     4801 aatatttata catttctacg tagtgtacac taatgtttgt acacttcttt atataccaaa
     4861 ttcgtacact attttataaa catcgttttg gacattatca cttgacaaaa attgttctct
     4921 ctttatgtat gtggtgtaca aaataggaag gtgtacaata ttgtagacgg cgcacgaggc
     4981 ggtgtatggt gtgacctatt atctagtcgt tttatgtata cctatataca ctatatttta
     5041 cacatctttg tacaccatca tattaggtag ttgtacacaa ctatatattt tcgtttgtac
     5101 ggtgacgtac actatcatat atggtatatc acttctcaca ttaatattat acattacttc
     5161 ttacattatc gtcgtacact actatttgta caccattttt tacactatcg tgtacaccgt
     5221 cggattgaga ttaaaaaaaa agtgttacgt attttcgaaa tataactaaa tttagacttt
     5281 ttagactcac ataaaagttt taaagattaa tttgattatg tggtaatttt tttatgaaaa
     5341 attactatca aataaagtaa aaatattgaa gtaaaaagaa gaaagataaa aagtaggtta
     5401 tgacaagatt tttttcttta tcttgttaca taacaaaaca ataaaaggtt atataagtta
     5461 tttactaaaa tctctattag acaaagaatt gtattataga caaaagagaa aagtgtctat
     5521 ttcacctatt tttaaaaaaa ctacctagtt tacaaattat ttttgaaaaa agtgtctagt
     5581 ttgccactta tctctaaaac taatccggtg gataaactaa ttaagattaa ggtgatgtga
     5641 atatacatga aatgacctta gtaaaatatt cctttcgcct ttgatatatt tgccatgtgc
     5701 ggaatcttta ttggcttcat agttacacaa atgttgcatg gaatttaaat tagagaagaa
     5761 accttttgat aacgttgggg atatcccaat cgtgccaacc caactattcc aattttacat
     5821 ttacatgtct aaaactaaaa ggttcagcaa gaaaaattat tttcgatgca aaaataaaca
     5881 ggtttagctt taaaagtatc aaacatataa taaaaaagtt tatataaaaa catacaatat
     5941 agtgtgaggc aagcattgtg cttttattta agttaatttt cattttttct tacgtgtttt
     6001 atagtattaa ttaccaatgg aagatttgac caaggaagtt agaaaggggg aaattttacc
     6061 cagattttta ttgttagtca acttgaatta attaactttc cagttttgaa ctattggaaa
     6121 ttataatatc agctttgacg cagaattaaa ggaaatacgt gtgtgactta ttaaaacaaa
     6181 acaaaaatgg gaccatcttt ttggtgttgt tgttcatatt agctcatttt atgtaagtaa
     6241 acttaatgag acttccatga ctataagtaa taaaaataaa aataaaaatt agcatgatga
     6301 tttaggaata atatctagag gttataatta gcataccttt acattaataa tttatttttt
     6361 ttccaaacct tgtataaatt agagctcata gacagctaca caaacaagaa gaaaaatagt
     6421 atatataaat aaataaaaat caatacgtga cataactcaa aattccaacc aacaattttt
     6481 ttttttggat ccccgggtgg tcagtccctt atgttacgtc ctgtagaaac cccaacccgt
     6541 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt
     6601 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt
     6661 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag
     6721 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg
     6781 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc
     6841 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc
     6901 accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc
     6961 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccggaatc
     7021 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg
     7081 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt
     7141 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact
     7201 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat
     7261 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc
     7321 atccggtcag tggcagtgaa gggccaacag ttcctgatta accacaaacc gttctacttt
     7381 actggctttg gtcgtcatga agatgcggac ttacgtggca aaggattcga taacgtgctg
     7441 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat
     7501 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat
     7561 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag
     7621 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag
     7681 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
     7741 gccaacgaac cggatacccg tccgcaagtg cacgggaata tttcgccact ggcggaagca
     7801 acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct
     7861 cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg
     7921 tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc
     7981 tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc
     8041 gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat
     8101 atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc
     8161 gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc
     8221 actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg
     8281 aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc tcctggcgca
     8341 ccatcgtcgg ctacagcctc gggaattgct accgagctcg aatttccccg atcgttcaaa
     8401 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     8461 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     8521 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     8581 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     8641 tcgggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8701 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8761 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc
     8821 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8881 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     8941 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9001 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9061 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt
     9121 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     9181 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt
     9241 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     9301 atcctgcca     
//
