LOCUS       GH985_pBI101_1_G        6215 bp    DNA        circular     04-AUG-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pBI101.1 from 1 to 6215
COMMENT     ID   PBI101     preliminary; DNA; SYN; 5350 BP.
COMMENT     XX
COMMENT     AC   U12639; IG1034; X04996; X04995; V00087; J01541; M61152;
            M38354;
COMMENT     AC   S54410; M14641;
COMMENT     XX
COMMENT     DT   01-FEB-1993 (Rel. 7, Created)
COMMENT     DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
COMMENT     XX
COMMENT     DE   Higher plants/E.coli plasmid vector pBI101 - incomplete.
COMMENT     XX
COMMENT     KW   cloning vector.
COMMENT     XX
COMMENT     OS   Cloning vector
COMMENT     OC   Artificial sequences; Cloning vehicles.
COMMENT     XX
COMMENT     RN   [1]
COMMENT     RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
COMMENT     RC   pBI121 from pBI101.1 & CMV 35S promoter
COMMENT     RC   pBI131 from pBI101.1 & tobacco rbcS promoter
COMMENT     RA   Jefferson R.A., Kavanaugh T.A., Bevan M.W.;
COMMENT     RT   "GUS fusions: beta-glucuronidase as a sensitive and versatile
            gene
COMMENT     RT   fusion marker in higher plants";
COMMENT     RL   EMBO J. 6:3901-3907(1987).
COMMENT     XX
COMMENT     RN   [2]
COMMENT     RP   1-5350
COMMENT     RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
COMMENT     RA   Wei W., Lindsey K.;
COMMENT     RT   "T-DNA sequence of the GUS gene fusion vector pBI101";
COMMENT     RL   Unpublished (1994).
COMMENT     XX
COMMENT     RN   [3]
COMMENT     RP   1-5350
COMMENT     RC   pBI101.1
COMMENT     RA   Wei W.;
COMMENT     RT   ;
COMMENT     RL   Submitted (22-JUL-1994) by:
COMMENT     RL   Wei W., Botany, University of Leicester, University Road,
COMMENT     RL   Leicester, LE1 7RH, UK.
COMMENT     XX
COMMENT     CC   NM (pBI101)
COMMENT     CC   CM (no)
COMMENT     CC   NA (ds-DNA)
COMMENT     CC   TP (linear)
COMMENT     CC   ST ()
COMMENT     CC   TY (plasmid)
COMMENT     CC   SP (CLONTECH)
COMMENT     CC   HO (E.coli)(plant)
COMMENT     CC   CP ()
COMMENT     CC   FN (expression)(reporter)
COMMENT     CC   SE ()
COMMENT     CC   PA (pBIN19)
COMMENT     CC   BR (pBI101.2)(pBI101.3)
COMMENT     CC   OF (pBI121)(pBI221)
COMMENT     CC   OR ()
COMMENT     XX
COMMENT     FH   Key             Location/Qualifiers
COMMENT     FH
COMMENT     XX
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             386..1180
                     /note="ANT E. coli neomycin phosphotransferase II gene
                     (NPTII); neomycin resistance gene (neo) E. coli kanamycin
                     resistance gene (kan)"
                     /locus_tag="ANT E. coli neomycin phosphotransferase II
                     gene (N"
                     /label="ANT E. coli neomycin phosphotransferase II gene
                     (N"
                     /ApEinfo_label="ANT E. coli neomycin phosphotransferase II
                     gene (N"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2504..3392
                     /locus_tag="ProGOXL4"
                     /label="ProGOXL4"
                     /ApEinfo_label="ProGOXL4"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3417..5225
                     /note="GEN E. coli beta-glucuronidase gene (GUS)"
                     /locus_tag="GEN E. coli beta-glucuronidase gene (GUS)"
                     /label="GEN E. coli beta-glucuronidase gene (GUS)"
                     /ApEinfo_label="GEN E. coli beta-glucuronidase gene (GUS)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           5296..5548
                     /note="TER 3'UTR of the nopaline synthase gene"
                     /locus_tag="TER 3'UTR of the nopaline synthase gene"
                     /label="TER 3'UTR of the nopaline synthase gene"
                     /ApEinfo_label="TER 3'UTR of the nopaline synthase gene"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_unit     6191..6215
                     /note="the left border repeat"
                     /locus_tag="the left border repeat"
                     /label="the left border repeat"
                     /ApEinfo_label="the left border repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
      181 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
      241 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
      301 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
      361 ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
      421 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
      481 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
      541 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
      601 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      661 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      721 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      781 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
      841 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
      901 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
      961 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     1021 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     1081 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     1141 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     1201 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     1261 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     1321 ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
     1861 ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
     1921 tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
     1981 aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
     2041 taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
     2101 tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
     2161 ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
     2221 tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
     2281 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2341 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     2401 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     2461 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgaggaag accttttttc
     2521 aaactgagct caggtgaact aaaatgacca ttttgacctt tacatgaaat atgtaatgaa
     2581 attaatttgg ttaaaccgag actaaaaccg gtcttaacac aaagcaagaa ctaaactgaa
     2641 tttaaaaagt cagcgttttg ttaacatgcc ataattgaat gctgtaattc aaaatttacg
     2701 gagacattct gccgagtatg tatatatgga gacattcttg aggctgagca atttactccc
     2761 aaagttccag cttttttgtt ttttagattt tgatcagccg gtctatggag acattcttaa
     2821 ggtgatattt ttgtaagtga gtgatatttt tgtcattttg atttataaac atataagaat
     2881 aaggaaatat ttgtctgata atttcttaag tttttttttt tgggcattct ctttgcaact
     2941 tgcggaatta tagaaatcat catgcaggtg tacattgttt tctctattca tttcatactt
     3001 atttattata tgtgttcctt acttatttat ttccaaatat tacgacaatt tcaaagagtg
     3061 atgataccag aatttagaaa gaaaatatta catcctttaa tttgattaaa actatctata
     3121 gtttgatgca attctactac gttttcttaa agactgtcta aaactccggc atgaatgtag
     3181 tagagtcaaa gagtttgctt gtccaaaatt ccaaattaaa tatataaaac aagaatcgtc
     3241 aatctaataa aaataacaat tttatgtgaa agaatatata attggacgta ttactccaac
     3301 catactactc ttcaacaaga aaatagataa accaacctct aacttaaaat tcaccatcaa
     3361 accaagaaca aaggaagaaa ccaccaacca ctggatcccc gggtggtcag tcccttatgt
     3421 tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact cgacggcctg tgggcattca
     3481 gtctggatcg cgaaaactgt ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa
     3541 gccgggcaat tgctgtgcca ggcagtttta acgatcagtt cgccgatgca gatattcgta
     3601 attatgcggg caacgtctgg tatcagcgcg aagtctttat accgaaaggt tgggcaggcc
     3661 agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg gtcaataatc
     3721 aggaagtgat ggagcatcag ggcggctata cgccatttga agccgatgtc acgccgtatg
     3781 ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg aactggcaga
     3841 ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttacttcc
     3901 atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc acgccgaaca
     3961 cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg
     4021 ttgactggca ggtggtggcc aatggtgatg tcagcgttga actgcgtgat gcggatcaac
     4081 aggtggttgc aactggacaa ggcactagcg ggactttgca agtggtgaat ccgcacctct
     4141 ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc cagacagagt
     4201 gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc agtgaagggc caacagttcc
     4261 tgattaacca caaaccgttc tactttactg gctttggtcg tcatgaagat gcggacttac
     4321 gtggcaaagg attcgataac gtgctgatgg tgcacgacca cgcattaatg gactggattg
     4381 gggccaactc ctaccgtacc tcgcattacc cttacgctga agagatgctc gactgggcag
     4441 atgaacatgg catcgtggtg attgatgaaa ctgctgctgt cggctttaac ctctctttag
     4501 gcattggttt cgaagcgggc aacaagccga aagaactgta cagcgaagag gcagtcaacg
     4561 gggaaactca gcaagcgcac ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc
     4621 acccaagcgt ggtgatgtgg agtattgcca acgaaccgga tacccgtccg caagtgcacg
     4681 ggaatatttc gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct
     4741 gcgtcaatgt aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc
     4801 tgtgcctgaa ccgttattac ggatggtatg tccaaagcgg cgatttggaa acggcagaga
     4861 aggtactgga aaaagaactt ctggcctggc aggagaaact gcatcagccg attatcatca
     4921 ccgaatacgg cgtggatacg ttagccgggc tgcactcaat gtacaccgac atgtggagtg
     4981 aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg
     5041 tcgtcggtga acaggtatgg aatttcgccg attttgcgac ctcgcaaggc atattgcgcg
     5101 ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa accgaagtcg gcggcttttc
     5161 tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac
     5221 aatgaatcaa caactctcct ggcgcaccat cgtcggctac agcctcggga attgctaccg
     5281 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     5341 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     5401 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     5461 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     5521 cgcgcggtgt catctatgtt actagatcgg gaattcactg gccgtcgttt tacaacgtcg
     5581 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5641 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5701 gaatggcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5761 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5821 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     5881 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5941 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     6001 tttcggaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     6061 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     6121 gaaaagaaaa accaccccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     6181 gcgtcaattt gtttacacca caatatatcc tgcca  
//
