LOCUS       GH986_pBI101_1_G        5930 bp    DNA        circular     04-AUG-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pBI101.1 from 1 to 6215
COMMENT     ID   PBI101     preliminary; DNA; SYN; 5350 BP.
COMMENT     XX
COMMENT     AC   U12639; IG1034; X04996; X04995; V00087; J01541; M61152;
            M38354;
COMMENT     AC   S54410; M14641;
COMMENT     XX
COMMENT     DT   01-FEB-1993 (Rel. 7, Created)
COMMENT     DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
COMMENT     XX
COMMENT     DE   Higher plants/E.coli plasmid vector pBI101 - incomplete.
COMMENT     XX
COMMENT     KW   cloning vector.
COMMENT     XX
COMMENT     OS   Cloning vector
COMMENT     OC   Artificial sequences; Cloning vehicles.
COMMENT     XX
COMMENT     RN   [1]
COMMENT     RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
COMMENT     RC   pBI121 from pBI101.1 & CMV 35S promoter
COMMENT     RC   pBI131 from pBI101.1 & tobacco rbcS promoter
COMMENT     RA   Jefferson R.A., Kavanaugh T.A., Bevan M.W.;
COMMENT     RT   "GUS fusions: beta-glucuronidase as a sensitive and versatile
            gene
COMMENT     RT   fusion marker in higher plants";
COMMENT     RL   EMBO J. 6:3901-3907(1987).
COMMENT     XX
COMMENT     RN   [2]
COMMENT     RP   1-5350
COMMENT     RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
COMMENT     RA   Wei W., Lindsey K.;
COMMENT     RT   "T-DNA sequence of the GUS gene fusion vector pBI101";
COMMENT     RL   Unpublished (1994).
COMMENT     XX
COMMENT     RN   [3]
COMMENT     RP   1-5350
COMMENT     RC   pBI101.1
COMMENT     RA   Wei W.;
COMMENT     RT   ;
COMMENT     RL   Submitted (22-JUL-1994) by:
COMMENT     RL   Wei W., Botany, University of Leicester, University Road,
COMMENT     RL   Leicester, LE1 7RH, UK.
COMMENT     XX
COMMENT     CC   NM (pBI101)
COMMENT     CC   CM (no)
COMMENT     CC   NA (ds-DNA)
COMMENT     CC   TP (linear)
COMMENT     CC   ST ()
COMMENT     CC   TY (plasmid)
COMMENT     CC   SP (CLONTECH)
COMMENT     CC   HO (E.coli)(plant)
COMMENT     CC   CP ()
COMMENT     CC   FN (expression)(reporter)
COMMENT     CC   SE ()
COMMENT     CC   PA (pBIN19)
COMMENT     CC   BR (pBI101.2)(pBI101.3)
COMMENT     CC   OF (pBI121)(pBI221)
COMMENT     CC   OR ()
COMMENT     XX
COMMENT     FH   Key             Location/Qualifiers
COMMENT     FH
COMMENT     XX
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             386..1180
                     /note="ANT E. coli neomycin phosphotransferase II gene
                     (NPTII); neomycin resistance gene (neo) E. coli kanamycin
                     resistance gene (kan)"
                     /locus_tag="ANT E. coli neomycin phosphotransferase II
                     gene (N"
                     /label="ANT E. coli neomycin phosphotransferase II gene
                     (N"
                     /ApEinfo_label="ANT E. coli neomycin phosphotransferase II
                     gene (N"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2504..3107
                     /locus_tag="ProGOXL5"
                     /label="ProGOXL5"
                     /ApEinfo_label="ProGOXL5"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3132..4940
                     /note="GEN E. coli beta-glucuronidase gene (GUS)"
                     /locus_tag="GEN E. coli beta-glucuronidase gene (GUS)"
                     /label="GEN E. coli beta-glucuronidase gene (GUS)"
                     /ApEinfo_label="GEN E. coli beta-glucuronidase gene (GUS)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           5011..5263
                     /note="TER 3'UTR of the nopaline synthase gene"
                     /locus_tag="TER 3'UTR of the nopaline synthase gene"
                     /label="TER 3'UTR of the nopaline synthase gene"
                     /ApEinfo_label="TER 3'UTR of the nopaline synthase gene"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_unit     5906..5930
                     /note="the left border repeat"
                     /locus_tag="the left border repeat"
                     /label="the left border repeat"
                     /ApEinfo_label="the left border repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
      181 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
      241 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
      301 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
      361 ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
      421 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
      481 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
      541 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
      601 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      661 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      721 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      781 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
      841 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
      901 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
      961 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     1021 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     1081 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     1141 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     1201 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     1261 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     1321 ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
     1861 ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
     1921 tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
     1981 aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
     2041 taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
     2101 tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
     2161 ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
     2221 tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
     2281 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2341 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     2401 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     2461 atttcacaca ggaaacagct atgaccatga ttacgccaag cttaaagatt tgaatcacct
     2521 gattgcttta ataaccagga actggggaga gagaaaagga gatgagaaga gtcaatagag
     2581 gattgagaat taacatagat tagacaacca ttgatttaaa tctctatggt catttctcca
     2641 aaaaacccaa ttatagattt tggatgcctt tgtttaatcc ttcttttctg gatattttca
     2701 gttatttttg ccttgatcta atattatttt tactgagata attacccaat agcaccttta
     2761 aaacttgagg taactatttt tgtatttact taattaatca tacatactac atatgattat
     2821 taaacgtagt atatatcaag tctctaggtc tattgaaaat aattatcata caagtaatta
     2881 aaaaaattcc ccatggggtc agaagctaat taatggcggg actctctgtt cccccaaaag
     2941 aatttgaaga catgactata aaaaaaaaag aatgaaacaa ttttattttg gactcctgat
     3001 ctgtgaaaga ctgaaacagt tcatacgcaa actgatataa aatccaaaat gttatctcta
     3061 agaatatggt ccgtgatcaa taatacagat ctaaaattca ccaacttgga tccccgggtg
     3121 gtcagtccct tatgttacgt cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg
     3181 gcctgtgggc attcagtctg gatcgcgaaa actgtggaat tgatcagcgt tggtgggaaa
     3241 gcgcgttaca agaaagccgg gcaattgctg tgccaggcag ttttaacgat cagttcgccg
     3301 atgcagatat tcgtaattat gcgggcaacg tctggtatca gcgcgaagtc tttataccga
     3361 aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag
     3421 tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg ctatacgcca tttgaagccg
     3481 atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat caccgtttgt gtgaacaacg
     3541 aactgaactg gcagactatc ccgccgggaa tggtgattac cgacgaaaac ggcaagaaaa
     3601 agcagtctta cttccatgat ttctttaact atgccggaat ccatcgcagc gtaatgctct
     3661 acaccacgcc gaacacctgg gtggacgata tcaccgtggt gacgcatgtc gcgcaagact
     3721 gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg tgatgtcagc gttgaactgc
     3781 gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac tagcgggact ttgcaagtgg
     3841 tgaatccgca cctctggcaa ccgggtgaag gttatctcta tgaactgtgc gtcacagcca
     3901 aaagccagac agagtgtgat atctacccgc ttcgcgtcgg catccggtca gtggcagtga
     3961 agggccaaca gttcctgatt aaccacaaac cgttctactt tactggcttt ggtcgtcatg
     4021 aagatgcgga cttacgtggc aaaggattcg ataacgtgct gatggtgcac gaccacgcat
     4081 taatggactg gattggggcc aactcctacc gtacctcgca ttacccttac gctgaagaga
     4141 tgctcgactg ggcagatgaa catggcatcg tggtgattga tgaaactgct gctgtcggct
     4201 ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa ctgtacagcg
     4261 aagaggcagt caacggggaa actcagcaag cgcacttaca ggcgattaaa gagctgatag
     4321 cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat tgccaacgaa ccggataccc
     4381 gtccgcaagt gcacgggaat atttcgccac tggcggaagc aacgcgtaaa ctcgacccga
     4441 cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat accatcagcg
     4501 atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa agcggcgatt
     4561 tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag aaactgcatc
     4621 agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac tcaatgtaca
     4681 ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac cgcgtctttg
     4741 atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt gcgacctcgc
     4801 aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac cgcaaaccga
     4861 agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt gaaaaaccgc
     4921 agcagggagg caaacaatga atcaacaact ctcctggcgc accatcgtcg gctacagcct
     4981 cgggaattgc taccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     5041 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     5101 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     5161 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     5221 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt
     5281 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5341 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5401 acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5461 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5521 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     5581 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5641 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     5701 aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac
     5761 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     5821 gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt
     5881 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca 
//
