LOCUS       GH987_pBI101_1_G        6429 bp    DNA        circular     04-AUG-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     ID   PBI101     preliminary; DNA; SYN; 5350 BP.
COMMENT     XX
COMMENT     AC   U12639; IG1034; X04996; X04995; V00087; J01541; M61152;
            M38354;
COMMENT     AC   S54410; M14641;
COMMENT     XX
COMMENT     DT   01-FEB-1993 (Rel. 7, Created)
COMMENT     DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
COMMENT     XX
COMMENT     DE   Higher plants/E.coli plasmid vector pBI101 - incomplete.
COMMENT     XX
COMMENT     KW   cloning vector.
COMMENT     XX
COMMENT     OS   Cloning vector
COMMENT     OC   Artificial sequences; Cloning vehicles.
COMMENT     XX
COMMENT     RN   [1]
COMMENT     RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
COMMENT     RC   pBI121 from pBI101.1 & CMV 35S promoter
COMMENT     RC   pBI131 from pBI101.1 & tobacco rbcS promoter
COMMENT     RA   Jefferson R.A., Kavanaugh T.A., Bevan M.W.;
COMMENT     RT   "GUS fusions: beta-glucuronidase as a sensitive and versatile
            gene
COMMENT     RT   fusion marker in higher plants";
COMMENT     RL   EMBO J. 6:3901-3907(1987).
COMMENT     XX
COMMENT     RN   [2]
COMMENT     RP   1-5350
COMMENT     RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
COMMENT     RA   Wei W., Lindsey K.;
COMMENT     RT   "T-DNA sequence of the GUS gene fusion vector pBI101";
COMMENT     RL   Unpublished (1994).
COMMENT     XX
COMMENT     RN   [3]
COMMENT     RP   1-5350
COMMENT     RC   pBI101.1
COMMENT     RA   Wei W.;
COMMENT     RT   ;
COMMENT     RL   Submitted (22-JUL-1994) by:
COMMENT     RL   Wei W., Botany, University of Leicester, University Road,
COMMENT     RL   Leicester, LE1 7RH, UK.
COMMENT     XX
COMMENT     CC   NM (pBI101)
COMMENT     CC   CM (no)
COMMENT     CC   NA (ds-DNA)
COMMENT     CC   TP (linear)
COMMENT     CC   ST ()
COMMENT     CC   TY (plasmid)
COMMENT     CC   SP (CLONTECH)
COMMENT     CC   HO (E.coli)(plant)
COMMENT     CC   CP ()
COMMENT     CC   FN (expression)(reporter)
COMMENT     CC   SE ()
COMMENT     CC   PA (pBIN19)
COMMENT     CC   BR (pBI101.2)(pBI101.3)
COMMENT     CC   OF (pBI121)(pBI221)
COMMENT     CC   OR ()
COMMENT     XX
COMMENT     FH   Key             Location/Qualifiers
COMMENT     FH
COMMENT     XX
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             386..1180
                     /note="ANT E. coli neomycin phosphotransferase II gene
                     (NPTII); neomycin resistance gene (neo) E. coli kanamycin
                     resistance gene (kan)"
                     /locus_tag="ANT E. coli neomycin phosphotransferase II
                     gene (N"
                     /label="ANT E. coli neomycin phosphotransferase II gene
                     (N"
                     /ApEinfo_label="ANT E. coli neomycin phosphotransferase II
                     gene (N"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3631..5439
                     /note="GEN E. coli beta-glucuronidase gene (GUS)"
                     /locus_tag="GEN E. coli beta-glucuronidase gene (GUS)"
                     /label="GEN E. coli beta-glucuronidase gene (GUS)"
                     /ApEinfo_label="GEN E. coli beta-glucuronidase gene (GUS)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           5510..5762
                     /note="TER 3'UTR of the nopaline synthase gene"
                     /locus_tag="TER 3'UTR of the nopaline synthase gene"
                     /label="TER 3'UTR of the nopaline synthase gene"
                     /ApEinfo_label="TER 3'UTR of the nopaline synthase gene"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_unit     6405..6429
                     /note="the left border repeat"
                     /locus_tag="the left border repeat"
                     /label="the left border repeat"
                     /ApEinfo_label="the left border repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2504..3606
                     /locus_tag="ProGOXL6"
                     /label="ProGOXL6"
                     /ApEinfo_label="ProGOXL6"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
      181 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
      241 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
      301 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
      361 ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
      421 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
      481 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
      541 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
      601 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      661 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      721 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      781 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
      841 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
      901 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
      961 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     1021 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     1081 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     1141 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     1201 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     1261 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     1321 ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
     1861 ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
     1921 tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
     1981 aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
     2041 taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
     2101 tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
     2161 ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
     2221 tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
     2281 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2341 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     2401 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     2461 atttcacaca ggaaacagct atgaccatga ttacgccaag cttagaaagc catgaaatta
     2521 gtgtacataa ataactaact aatggagatt acttgtattt tctaaaaccg aagtatatta
     2581 cggaaatcca gactaatctc aagagacatg taaaaacccg gtattcttgt cactactggt
     2641 gtttaccgtg gccaaaaggt taactacaat gattttcata gagagtgtta ctcgatcctc
     2701 gactttgaca tgtatggtga cttggtgagt cctcgacaaa ttcaagagac cattggaaca
     2761 cacactattg gtcacgacta tattacgaga aagattgaat gttatggagt taaggataat
     2821 atgtatttag aagtctttga ttatgtaagt tagattgttg ttagttttca atgcatggtg
     2881 ttttatgttt acactatgtt tgataaagaa agtttatgtt cttaaactac aattacttta
     2941 agtacaatct tgttcacttt gaaaaataat agggtcctac acaattatac gtcaccgcaa
     3001 ttatttatct ttatagacat tgctgttttc agtttcataa agactaacac tagatatata
     3061 gtcacttccc tcaacaagga aaacaattat caacgtcggg acagatctac tctgatgctt
     3121 gttgatgtgc aagtccactg cagccaatta aatgttaaca atgtccgtga catgcatagt
     3181 cattattgtt aagttggctc atttgggaat gtgttaatca tctattaatt atgttagttg
     3241 agagtttgtt gccaatcacg tttctttatt acttttcaaa atatgtgtaa tatgtatagt
     3301 tatgtgtacg tattatgcaa tcctttaccc tttggatcag ccccatgatg gtttgtaagg
     3361 aaatggtcct taactttatg acatcaaatt aagaaattgt ctgcatgacc acgtcattgt
     3421 aaaaaaaagg gcacgagaga cagatacgaa acaccaatgt tttcttctat cacagaaact
     3481 ccacaaatct ataggctgtt ttggggagac gtcttcttac cacacattta aaaatagcaa
     3541 ttgctaataa taaacgtaag tgaaataaca acacctataa atcacgcaga gacaagagct
     3601 tgttctggat ccccgggtgg tcagtccctt atgttacgtc ctgtagaaac cccaacccgt
     3661 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt
     3721 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt
     3781 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag
     3841 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg
     3901 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc
     3961 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc
     4021 accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc
     4081 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccggaatc
     4141 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg
     4201 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt
     4261 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact
     4321 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat
     4381 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc
     4441 atccggtcag tggcagtgaa gggccaacag ttcctgatta accacaaacc gttctacttt
     4501 actggctttg gtcgtcatga agatgcggac ttacgtggca aaggattcga taacgtgctg
     4561 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat
     4621 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat
     4681 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag
     4741 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag
     4801 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
     4861 gccaacgaac cggatacccg tccgcaagtg cacgggaata tttcgccact ggcggaagca
     4921 acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct
     4981 cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg
     5041 tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc
     5101 tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc
     5161 gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat
     5221 atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc
     5281 gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc
     5341 actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg
     5401 aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc tcctggcgca
     5461 ccatcgtcgg ctacagcctc gggaattgct accgagctcg aatttccccg atcgttcaaa
     5521 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     5581 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     5641 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     5701 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     5761 tcgggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     5821 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     5881 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc
     5941 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6001 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     6061 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6121 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6181 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt
     6241 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     6301 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt
     6361 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     6421 atcctgcca     
//
