LOCUS       GH999_LY31_pGree       11556 bp    DNA        circular     04-AUG-2021
DEFINITION  Agrobacterium binary vector with kanamycin-and
            bialophos/phosphinothricin-resistance genes.
ACCESSION   .
VERSION     .
KEYWORDS    pGreenII 0229
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.
  TITLE     pGreen: a versatile and flexible binary Ti vector for
            Agrobacterium-mediated plant transformation.
  JOURNAL   Plant Mol. Biol. 2000;42:819-32.
  PUBMED    10890530
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   pGreen website
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, 10 Feb, 2016 from SnapGene Viewer 3.0.3
            http://www.snapgene.com
COMMENT     This plasmid must be transformed into an Agrobacterium strain that
            also carries a vector such as pSoup.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      63..498
                     /note="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
                     /locus_tag="pSa ori"
                     /label="pSa ori"
                     /ApEinfo_label="pSa ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    633..655
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
                     /locus_tag="LB T-DNA repeat"
                     /label="LB T-DNA repeat"
                     /ApEinfo_label="LB T-DNA repeat"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      679..930
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
                     /locus_tag="NOS terminator"
                     /label="NOS terminator"
                     /ApEinfo_label="NOS terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(950..1501)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /note="BlpR"
                     /note="confers resistance to bialophos or
                     phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIQTSTVNFRTEPQEPQ
                     E
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLG
                     S
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVG
                     F WQLDFSLPVPPRPVLPVTEI"
                     /locus_tag="phosphinothricin acetyltransferase"
                     /label="phosphinothricin acetyltransferase"
                     /ApEinfo_label="phosphinothricin acetyltransferase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(1544..1721)
                     /note="NOS promoter"
                     /note="nopaline synthase promoter"
                     /locus_tag="NOS promoter"
                     /label="NOS promoter"
                     /ApEinfo_label="NOS promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1968..1984
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 fwd"
                     /label="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1991..2009
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(9246..9264)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(9285..9301)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    9309..9325
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
                     /label="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(9333..9364)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9639..9663
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
                     /locus_tag="RB T-DNA repeat"
                     /label="RB T-DNA repeat"
                     /ApEinfo_label="RB T-DNA repeat"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(9754..10342)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(10513..11328)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     G
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     K
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     A
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     I ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /locus_tag="aminoglycoside phosphotransferase"
                     /label="aminoglycoside phosphotransferase"
                     /ApEinfo_label="aminoglycoside phosphotransferase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8976..9227
                     /note="NOSt (clone first)"
                     /locus_tag="NOSt"
                     /label="NOSt"
                     /ApEinfo_label="NOSt"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8199..8967
                     /locus_tag="Citrine"
                     /label="Citrine"
                     /ApEinfo_label="Citrine"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2024..6501
                     /locus_tag="RUBYp with 5'UTR"
                     /label="RUBYp with 5'UTR"
                     /ApEinfo_label="RUBYp with 5'UTR"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6508..8148
                     /locus_tag="GOXL4"
                     /label="GOXL4"
                     /ApEinfo_label="GOXL4"
                     /ApEinfo_fwdcolor="#a5095a"
                     /ApEinfo_revcolor="#a5095a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc
      541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct
      601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta
      661 tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
      721 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
      781 tcaaaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
      841 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
      901 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat cagatttcgg
      961 tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca
     1021 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccacgg ggggcatatc
     1081 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc
     1141 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc
     1201 ccgtccgctg gtggcggggg gagacgtaca cggttgactc ggccgtccag tcgtaggcgt
     1261 tgcgtgcctt ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga
     1321 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct
     1381 gcggctcggt acggaagttg accgtgcttg tctggatgta gtggttgacg atggtgcaga
     1441 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca
     1501 tggtagatcc ccctcgatcg agttgagagt gaatatgaga ctctaattgg ataccgaggg
     1561 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac
     1621 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac
     1681 tccagaaacc cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa
     1741 acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctcatgtatc
     1801 gataacatta acgtttacaa tttcgcgcca ttcgccattc aggctgcgca actgttggga
     1861 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1921 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1981 cagtgaattg taatacgact cactataggg cgaattgggt accgctaacc caatctcaat
     2041 cgaaccggac caaattagaa tgaaatggtt ttagtgagtt acatggttct taatttgact
     2101 ggatgaaatt aattaaaggc actatataga tgaaactaaa actacaagaa cattgctcta
     2161 acttttacac aactttaaaa aaaaaaaaaa aaaaaatcgt ggaatgttga ttcatttgac
     2221 ctttattctg gattgaaagt gatgcaaaaa tgactttatc taataaataa aaaaaagaag
     2281 actttatctg atgtagtgaa attcttgtat ttgttgatgc aatgatgcta tggattgttt
     2341 tcaaatgttt caattacgta tcaaattatt ttatttgtac caacacatgc aagtatgcaa
     2401 ccatggcctc gtcgtaaaga actttgggca acatacaaat ttgggcaaac atctataaca
     2461 cattggattt ttttgatcga tttaatttga ttcttggaat tactattacc ttcttctttt
     2521 aaaaattcag taagatatgt gttaccgtgt gaaagtctat atgtgtggga acgaaaggtt
     2581 tgaagacgag taagaaagta gaagagcaca atgcgaaact ttattggtat acgtaactgt
     2641 ttattgacga catttgtagg aacaaaaatt gcaagataac acatttttta gtgtttgctt
     2701 tgcttgttta tagtaaccgg taggcagtgg cggaggcaga ataattagat acaggggtca
     2761 ctctttttgt aagccaaata ccaaaaaaaa aaaattaaga aaaatgataa tatcctcata
     2821 aatgataatt tcaaaaaaaa atatatatat gagaaagaaa aaaactgttc tagtaaagta
     2881 aacattaaat ctaaagtaat ttatactgtg ttaaacaata ataatgagat taacttagtt
     2941 taatagactt atttttttat ttataaaatt aattgggtta atttgttgta ggtttaatat
     3001 tttttgttag actatatttg aatatatatt gatgggggtc aataatattt tgggaaaggg
     3061 tcaatttata aaaattaaca aaacctaaag taattttcta aaatccatag gggtcaattg
     3121 acccccatga ttatacactc cctccgccac tgccggtagg gtttgatgtc ttggattgtt
     3181 aagttttaaa gttttgtctc atgatctatg taagtgctta agccggcgct agcataaatt
     3241 gaacctttaa tcagaatgta gtagttgacc tggatggatg acacttttat gcgagatggg
     3301 tcgagcgacg ctaaccatta ggccctcgat cggcagtcgg cagatataac ttagatacag
     3361 tggatagtga catatcctct taataaatat gttttaaatg ttagattaac ttatgaccta
     3421 aagaaaagaa gttagattaa cattttttag aaattattat ttcggatagg tttaataagc
     3481 ataagtcagc gaaacttata aggtatttat ttacaattat aaaatcaaca gcttccaaac
     3541 aaaatctttt ttctttttga cttcttaaaa caaaatctca tactaaccga tttaacatta
     3601 tgtttgtgaa taatatggta acgcgaatga ttagcaacat aaaaaacaaa gagtcaacta
     3661 accgcattca tgaatggaat ggaatgtcac ggtcaatcat tagatttggt gttacggaat
     3721 acatatttat taaatgtata tgaagaaaaa agcatggcca ataataatgt aaataaattg
     3781 gctcaacatg cgatgtatgt acataattta gagtggaaaa tgaaaagata tatacaatgt
     3841 gtattaatat agatgtacgc agttgtataa aattgatgga catgatttag gttttttttt
     3901 aaaaaattga tggacatttt tcaaatatca aaatgtttaa tttatatttt ctacatataa
     3961 ttgaaattaa acaaaggaga tataagttct acataatttt actttgtatc gttggaacaa
     4021 acacgtacag tagtattatt ttatatatgt gcatgtatgt tttgcgttcc tttaaaaatc
     4081 aaatacatgg gaagataaca tgcaacgaac cagatgatac agcgcctgat gagaggtcaa
     4141 aaacgtaacg agtttgactt gtataatatt aatatgtagt tgcatgtttg atgttgacac
     4201 agcatcgcca tcattaattc gttctctgca gtctaccgaa ctaattaatc tcaaataatc
     4261 gatgagtaca tatatgttaa gtccgtcgag gctaatgtat aattcaataa tataaatatg
     4321 ttcattcgag gatatagttc atattattat atatctgttt aaaattattg actccaatca
     4381 tcctataaac taagagggtt gacaccgcag caaagaaaaa aataaaatat accataattc
     4441 atatatttgc acaatctgaa ttataatgga aattaagttt gctcaaacaa aaaaaaaggg
     4501 ttggctccat atacgaaatt gttaatttgt aattttgtag gacttctaat taaaaaaata
     4561 tatatcaata aatgattatt ttatatatgt caattatatc tgatcattaa ttaattaatt
     4621 gtttcgaaaa taattaatta attttgaaat ttaaattaaa gcaatattaa atgtaaaaat
     4681 tttaaattat attaaactag aaatttattg tgagcatttt tgtgtataga tcaagatatg
     4741 atgaatcttg tgggattttt tttattctat aacaggtgtt gtttcatatg ctacgcattt
     4801 ttatatattt aaccaaacat aatgacaagt tgacaacgac tagtatttct tcccatttag
     4861 atgataagat tagtccaaat actccaaacc gtctgttgga ttaagaatga ccggaatacg
     4921 attactccaa aacgcatatg ggtttactgg tcgagaatat acctttggat taagatcatg
     4981 tagagttatt tcattacgat tttgatttct tttggttatt gtgaatatgt tttttacttt
     5041 cttttttctg ttagagatca tttgtgaata tgttaagtgg taagtgtaca atttttcata
     5101 ttttgaatcg agtatcataa atatcattgt acacatacca gtttatttat ttcgtcctga
     5161 tattaacaag taacaacgtc aattttgaga tcaattattt gaaaatatag aaaactgaaa
     5221 gtattaacca atttagtttg attctaatta gagtcatcgt attgtgctag taatcggtga
     5281 cgggaaccac ataaaacgac agtttatcaa cataaaaaaa aatcacaaac acatagaaga
     5341 ctctagtttt gttataaact tgtgctaaat aattaacacg atacataata ctcttcatta
     5401 ttatatatac ttttcatgta taataatgca tggacattac acaaatggaa tgaatagtac
     5461 aattgtatgt gcatgaatgt ttttctaaaa actgacatcc gtttgtatgc tattttatcc
     5521 ttgtttgtta tttaatagta gaaaacttgc gaggaccaaa ataattagca tgcaaagaac
     5581 cagaagaaat cgtgagaact aaaacgtaaa gaactcttgt gacttgtata atatatagtt
     5641 tgtatgcatg tttgatgttg acacaacata tctccatcat taattcgtcc cttgcagtct
     5701 ctcaaactta ttaatctcaa ataatcgatg tttatagatt aagtccctcg agactaataa
     5761 tgtaaatcca atgatacaat gtggttcctt cgacgacgaa tcatattctg atttctgtat
     5821 atctgtaata taaagtacga ttattattat atttgaatac aatcatactt aataaaatat
     5881 tgtgtcatat atatttatct ccataaatgt ttctcttgac tgaaatgtga atacccaaac
     5941 ataaaaacat atacagtaaa tactttgttc aattcccatt aaatttctct ttgaatctaa
     6001 aatcagacat agaaatactg ttgtttagag aaagattttg aatatataga acattaaatc
     6061 gccgcagaac tttgttgttt agagaaagat tttaaatatg ataagaactc aactaaagaa
     6121 aaagaaaaaa aattatgacg tctcaattta gagttttatt ggttcttttg tacagttctg
     6181 taatgttagg aatcgtttta tacacaaagg taacttaaat ctttcaaacg tctacggtag
     6241 tggacggtca ttaaaatcag agttaagaaa acgaacaaac caaatttaat catatcttat
     6301 acactcgaga ccttgattat atattactct tttatatatt aacagaacta gaaataaaaa
     6361 ctatacatta ttagaatatc gtaacgtata ctaatatccc atcgaccagt atataaacga
     6421 cacaagtcac agtacattct catcacatac cacaaaatat ctcataaaga tcaaataaac
     6481 actaaacctg aaagcaaaaa agggcccATG ATCAACTCCA AAAATACATT CATCGTCGCT
     6541 ACAACCATCT TATGCCTCTC CATGGCCATA TTATCCGAAG GTCAAGCTAA CCCTTTTCTA
     6601 TTGCAACTCG ATCGGTGGGA AATGTTGCTA CCGAGCATCG GTATATCCGC CATGCACATG
     6661 CAACTCCTCC ACAACGGCAT GGTTATAATG TTCGACCGTA CCGATTTCGG TACCTCAAAT
     6721 GTTTCTCTAC CCGGAGGCAT ATGTCGTTAC GACCCTACTG ACACCGCAGA AAAGTTCGAT
     6781 TGCAGCGCAC ACTCAGTTCT CTACGATGTT GTCTCCAACA CATACCGTCC TTTGAATGTC
     6841 CAAACAGATA CATGGTGTTC TTCTGGTGCG GTTCTTCCCA ACGGAACATT GGTTCAAACC
     6901 GGTGGATACA ACGATGGTGA GCGTGCTGCT CGAATGTTTA GCCCTTGTGG TTATAGTGAT
     6961 ACATGTGATT GGATTGAGTT TCCTCAGTAT CTATCCCAAC GTAGATGGTA TGCCACCAAC
     7021 CAAATATTAC CCGATGGACG GATCATTGTG GTCGGAGGAA GAAGACAGTT CAACTATGAA
     7081 CTTTTTCCCC GACATGATTC TCGGTCTAGA AGCTCAAGAC TTGAGTTTCT TAGAGAAACT
     7141 TCAGATGGAA GTAACGAGAA CAACTTATAT CCGTTCATAC ATCTTTTACC TGATGGAAAC
     7201 TTATTTGTCT TCGCTAACAC AAGATCCATT GTGTTCGATT ACAAAAAGAA CCGAATCGTG
     7261 AAAGAGTTTC CAGAGATTCC AGGTGGTGAT CCAAGGAACT ATCCAAGCAG TGGTTCTTCT
     7321 ATACTATTTC CATTAGATGA TACCAACGAT GCTAACGTAG AAGTTGAGAT AATGGTATGT
     7381 GGAGGCTCTC CTAAAGGCGG CTTCTCACGT GGATTCACAC GTGCCACCTC CACATGCGGG
     7441 AGGCTAAAAC TATCCGATCA GAGTCCAAGT TGGGAAATGG AAACCATGCC ATTGCCACGT
     7501 GTTATGGGAG ACATGCTACT CTTACCAACA GGTGACGTCA TCATCGTAAA CGGAGCCGGA
     7561 GCTGGCACGG CCGGTTGGGA GAAAGCACGT GACCCGATTA TCCAACCTGT GATATATCAA
     7621 CCGTTTGATC ATCTCTTCAC CGTTATGTCC ACACCATCAC GTCCTAGGAT GTACCATTCC
     7681 TCAGCGATTT TATTACCAGA CGGTCGGGTT TTAGTTGGTG GAAGTAATCC TCACGTTTAT
     7741 TACAACTTTA CCAACGTCGA GTATCCAACG GATTTAAGCC TCGAGGCTTA TTCTCCACCA
     7801 TATCTATTTT TCACATCCGA CCCAATACGT CCCAAGATAT TATTGACAAG CGACAAAGTA
     7861 TTGAGTTACA AGAGATTGTT TAACGTGGAT TTCTCCATAG CTCAGTTTCT GACCGTTGAT
     7921 CTTCTCTCTG TGAGGATAGT TGCACCATCT TTCACTACAC ACTCCTTTGC GATGAATCAG
     7981 AGGATGGTAA TCTTGAAGCT ACTCTCTGTG ACGCGTGACC AGCTAACAAA TTCTTATAGA
     8041 GTCAGTGCAT TGGGTCCATC AACGGCTGAG ATTGCTCCTC CGGGATACTA TATGATATTT
     8101 CTGGTGCATG CAGGTATACC AAGCTCAGCC GCTTGGGTGC AGATTGAGGG aagcttgata
     8161 tcgaattcct gcagcccggg ggatccacta gttctagagC CTGGAGGTGG AGGTGGAGCT
     8221 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
     8281 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
     8341 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
     8401 GTGACCACCT TCGGCTACGG CCTGATGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG
     8461 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
     8521 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
     8581 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
     8641 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
     8701 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
     8761 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
     8821 CTGAGCTACC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
     8881 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGATCCT
     8941 GCTGGTGCTG CTGCGCCTCT AGCATAAgcg gccgcgatcg ttcaaacatt tggcaataaa
     9001 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     9061 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     9121 tttgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     9181 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgag ctccagcttt
     9241 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
     9301 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag hcataaagtg
     9361 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     9421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9481 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9601 agaatcaggg gataacgcag gaaagaacat gaaggccttg acaggatata ttggcgggta
     9661 aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc
     9721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    10201 atttggtatc tgcgctctgc tgaagccagt taccttcgga agaagagttg gtagctcttg
    10261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgtgtaaca
    10501 ttggtctagt gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
    10561 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
    10621 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
    10681 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
    10741 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
    10801 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
    10861 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
    10921 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
    10981 tgaatcagga tattcttcta atacctggaa tgctgttttc cctgggatcg cagtggtgag
    11041 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
    11101 cgtcagccag tttagtctga ccatctcatc tgtaacaaca ttggcaacgc tacctttgcc
    11161 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc
    11221 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
    11281 atttaatcgc ggccttgagc aagacgtttc ccgttgaata tggctcataa caccccttgt
    11341 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
    11401 aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc
    11461 tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca
    11521 aaagttcaaa atcaccaact ggtccaccta caacaa  
//
