LOCUS       GH1000_LY32_pGre       11607 bp    DNA        circular     04-AUG-2021
DEFINITION  Agrobacterium binary vector with kanamycin-and
            bialophos/phosphinothricin-resistance genes.
ACCESSION   .
VERSION     .
KEYWORDS    pGreenII 0229
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.
  TITLE     pGreen: a versatile and flexible binary Ti vector for
            Agrobacterium-mediated plant transformation.
  JOURNAL   Plant Mol. Biol. 2000;42:819-32.
  PUBMED    10890530
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   pGreen website
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, 10 Feb, 2016 from SnapGene Viewer 3.0.3
            http://www.snapgene.com
COMMENT     This plasmid must be transformed into an Agrobacterium strain that
            also carries a vector such as pSoup.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      63..498
                     /note="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
                     /locus_tag="pSa ori"
                     /label="pSa ori"
                     /ApEinfo_label="pSa ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    633..655
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
                     /locus_tag="LB T-DNA repeat"
                     /label="LB T-DNA repeat"
                     /ApEinfo_label="LB T-DNA repeat"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      679..930
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
                     /locus_tag="NOS terminator"
                     /label="NOS terminator"
                     /ApEinfo_label="NOS terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(950..1501)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /note="BlpR"
                     /note="confers resistance to bialophos or
                     phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIQTSTVNFRTEPQEPQ
                     E
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLG
                     S
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVG
                     F WQLDFSLPVPPRPVLPVTEI"
                     /locus_tag="phosphinothricin acetyltransferase"
                     /label="phosphinothricin acetyltransferase"
                     /ApEinfo_label="phosphinothricin acetyltransferase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(1544..1721)
                     /note="NOS promoter"
                     /note="nopaline synthase promoter"
                     /locus_tag="NOS promoter"
                     /label="NOS promoter"
                     /ApEinfo_label="NOS promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1968..1984
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 fwd"
                     /label="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1991..2009
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(9297..9315)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(9336..9352)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    9360..9376
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
                     /label="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(9384..9415)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9690..9714
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
                     /locus_tag="RB T-DNA repeat"
                     /label="RB T-DNA repeat"
                     /ApEinfo_label="RB T-DNA repeat"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(9805..10393)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(10564..11379)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     G
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     K
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     A
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     I ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /locus_tag="aminoglycoside phosphotransferase"
                     /label="aminoglycoside phosphotransferase"
                     /ApEinfo_label="aminoglycoside phosphotransferase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9027..9278
                     /note="NOSt (clone first)"
                     /locus_tag="NOSt"
                     /label="NOSt"
                     /ApEinfo_label="NOSt"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8250..9018
                     /locus_tag="Citrine"
                     /label="Citrine"
                     /ApEinfo_label="Citrine"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2024..6501
                     /locus_tag="RUBYp with 5'UTR"
                     /label="RUBYp with 5'UTR"
                     /ApEinfo_label="RUBYp with 5'UTR"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6508..8199
                     /locus_tag="GOXL5"
                     /label="GOXL5"
                     /ApEinfo_label="GOXL5"
                     /ApEinfo_fwdcolor="#a5095a"
                     /ApEinfo_revcolor="#a5095a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc
      541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct
      601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta
      661 tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
      721 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
      781 tcaaaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
      841 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
      901 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat cagatttcgg
      961 tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca
     1021 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccacgg ggggcatatc
     1081 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc
     1141 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc
     1201 ccgtccgctg gtggcggggg gagacgtaca cggttgactc ggccgtccag tcgtaggcgt
     1261 tgcgtgcctt ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga
     1321 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct
     1381 gcggctcggt acggaagttg accgtgcttg tctggatgta gtggttgacg atggtgcaga
     1441 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca
     1501 tggtagatcc ccctcgatcg agttgagagt gaatatgaga ctctaattgg ataccgaggg
     1561 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac
     1621 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac
     1681 tccagaaacc cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa
     1741 acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctcatgtatc
     1801 gataacatta acgtttacaa tttcgcgcca ttcgccattc aggctgcgca actgttggga
     1861 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1921 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1981 cagtgaattg taatacgact cactataggg cgaattgggt accgctaacc caatctcaat
     2041 cgaaccggac caaattagaa tgaaatggtt ttagtgagtt acatggttct taatttgact
     2101 ggatgaaatt aattaaaggc actatataga tgaaactaaa actacaagaa cattgctcta
     2161 acttttacac aactttaaaa aaaaaaaaaa aaaaaatcgt ggaatgttga ttcatttgac
     2221 ctttattctg gattgaaagt gatgcaaaaa tgactttatc taataaataa aaaaaagaag
     2281 actttatctg atgtagtgaa attcttgtat ttgttgatgc aatgatgcta tggattgttt
     2341 tcaaatgttt caattacgta tcaaattatt ttatttgtac caacacatgc aagtatgcaa
     2401 ccatggcctc gtcgtaaaga actttgggca acatacaaat ttgggcaaac atctataaca
     2461 cattggattt ttttgatcga tttaatttga ttcttggaat tactattacc ttcttctttt
     2521 aaaaattcag taagatatgt gttaccgtgt gaaagtctat atgtgtggga acgaaaggtt
     2581 tgaagacgag taagaaagta gaagagcaca atgcgaaact ttattggtat acgtaactgt
     2641 ttattgacga catttgtagg aacaaaaatt gcaagataac acatttttta gtgtttgctt
     2701 tgcttgttta tagtaaccgg taggcagtgg cggaggcaga ataattagat acaggggtca
     2761 ctctttttgt aagccaaata ccaaaaaaaa aaaattaaga aaaatgataa tatcctcata
     2821 aatgataatt tcaaaaaaaa atatatatat gagaaagaaa aaaactgttc tagtaaagta
     2881 aacattaaat ctaaagtaat ttatactgtg ttaaacaata ataatgagat taacttagtt
     2941 taatagactt atttttttat ttataaaatt aattgggtta atttgttgta ggtttaatat
     3001 tttttgttag actatatttg aatatatatt gatgggggtc aataatattt tgggaaaggg
     3061 tcaatttata aaaattaaca aaacctaaag taattttcta aaatccatag gggtcaattg
     3121 acccccatga ttatacactc cctccgccac tgccggtagg gtttgatgtc ttggattgtt
     3181 aagttttaaa gttttgtctc atgatctatg taagtgctta agccggcgct agcataaatt
     3241 gaacctttaa tcagaatgta gtagttgacc tggatggatg acacttttat gcgagatggg
     3301 tcgagcgacg ctaaccatta ggccctcgat cggcagtcgg cagatataac ttagatacag
     3361 tggatagtga catatcctct taataaatat gttttaaatg ttagattaac ttatgaccta
     3421 aagaaaagaa gttagattaa cattttttag aaattattat ttcggatagg tttaataagc
     3481 ataagtcagc gaaacttata aggtatttat ttacaattat aaaatcaaca gcttccaaac
     3541 aaaatctttt ttctttttga cttcttaaaa caaaatctca tactaaccga tttaacatta
     3601 tgtttgtgaa taatatggta acgcgaatga ttagcaacat aaaaaacaaa gagtcaacta
     3661 accgcattca tgaatggaat ggaatgtcac ggtcaatcat tagatttggt gttacggaat
     3721 acatatttat taaatgtata tgaagaaaaa agcatggcca ataataatgt aaataaattg
     3781 gctcaacatg cgatgtatgt acataattta gagtggaaaa tgaaaagata tatacaatgt
     3841 gtattaatat agatgtacgc agttgtataa aattgatgga catgatttag gttttttttt
     3901 aaaaaattga tggacatttt tcaaatatca aaatgtttaa tttatatttt ctacatataa
     3961 ttgaaattaa acaaaggaga tataagttct acataatttt actttgtatc gttggaacaa
     4021 acacgtacag tagtattatt ttatatatgt gcatgtatgt tttgcgttcc tttaaaaatc
     4081 aaatacatgg gaagataaca tgcaacgaac cagatgatac agcgcctgat gagaggtcaa
     4141 aaacgtaacg agtttgactt gtataatatt aatatgtagt tgcatgtttg atgttgacac
     4201 agcatcgcca tcattaattc gttctctgca gtctaccgaa ctaattaatc tcaaataatc
     4261 gatgagtaca tatatgttaa gtccgtcgag gctaatgtat aattcaataa tataaatatg
     4321 ttcattcgag gatatagttc atattattat atatctgttt aaaattattg actccaatca
     4381 tcctataaac taagagggtt gacaccgcag caaagaaaaa aataaaatat accataattc
     4441 atatatttgc acaatctgaa ttataatgga aattaagttt gctcaaacaa aaaaaaaggg
     4501 ttggctccat atacgaaatt gttaatttgt aattttgtag gacttctaat taaaaaaata
     4561 tatatcaata aatgattatt ttatatatgt caattatatc tgatcattaa ttaattaatt
     4621 gtttcgaaaa taattaatta attttgaaat ttaaattaaa gcaatattaa atgtaaaaat
     4681 tttaaattat attaaactag aaatttattg tgagcatttt tgtgtataga tcaagatatg
     4741 atgaatcttg tgggattttt tttattctat aacaggtgtt gtttcatatg ctacgcattt
     4801 ttatatattt aaccaaacat aatgacaagt tgacaacgac tagtatttct tcccatttag
     4861 atgataagat tagtccaaat actccaaacc gtctgttgga ttaagaatga ccggaatacg
     4921 attactccaa aacgcatatg ggtttactgg tcgagaatat acctttggat taagatcatg
     4981 tagagttatt tcattacgat tttgatttct tttggttatt gtgaatatgt tttttacttt
     5041 cttttttctg ttagagatca tttgtgaata tgttaagtgg taagtgtaca atttttcata
     5101 ttttgaatcg agtatcataa atatcattgt acacatacca gtttatttat ttcgtcctga
     5161 tattaacaag taacaacgtc aattttgaga tcaattattt gaaaatatag aaaactgaaa
     5221 gtattaacca atttagtttg attctaatta gagtcatcgt attgtgctag taatcggtga
     5281 cgggaaccac ataaaacgac agtttatcaa cataaaaaaa aatcacaaac acatagaaga
     5341 ctctagtttt gttataaact tgtgctaaat aattaacacg atacataata ctcttcatta
     5401 ttatatatac ttttcatgta taataatgca tggacattac acaaatggaa tgaatagtac
     5461 aattgtatgt gcatgaatgt ttttctaaaa actgacatcc gtttgtatgc tattttatcc
     5521 ttgtttgtta tttaatagta gaaaacttgc gaggaccaaa ataattagca tgcaaagaac
     5581 cagaagaaat cgtgagaact aaaacgtaaa gaactcttgt gacttgtata atatatagtt
     5641 tgtatgcatg tttgatgttg acacaacata tctccatcat taattcgtcc cttgcagtct
     5701 ctcaaactta ttaatctcaa ataatcgatg tttatagatt aagtccctcg agactaataa
     5761 tgtaaatcca atgatacaat gtggttcctt cgacgacgaa tcatattctg atttctgtat
     5821 atctgtaata taaagtacga ttattattat atttgaatac aatcatactt aataaaatat
     5881 tgtgtcatat atatttatct ccataaatgt ttctcttgac tgaaatgtga atacccaaac
     5941 ataaaaacat atacagtaaa tactttgttc aattcccatt aaatttctct ttgaatctaa
     6001 aatcagacat agaaatactg ttgtttagag aaagattttg aatatataga acattaaatc
     6061 gccgcagaac tttgttgttt agagaaagat tttaaatatg ataagaactc aactaaagaa
     6121 aaagaaaaaa aattatgacg tctcaattta gagttttatt ggttcttttg tacagttctg
     6181 taatgttagg aatcgtttta tacacaaagg taacttaaat ctttcaaacg tctacggtag
     6241 tggacggtca ttaaaatcag agttaagaaa acgaacaaac caaatttaat catatcttat
     6301 acactcgaga ccttgattat atattactct tttatatatt aacagaacta gaaataaaaa
     6361 ctatacatta ttagaatatc gtaacgtata ctaatatccc atcgaccagt atataaacga
     6421 cacaagtcac agtacattct catcacatac cacaaaatat ctcataaaga tcaaataaac
     6481 actaaacctg aaagcaaaaa agggcccATG ACGCAAGAAA GATTCAAGAA CAACCTTAAC
     6541 GCCGTCGTGA TATCATTCTT CTTCTTCTTC CTCTGTTCCA CGTCGGATTT GCTCCTTCCA
     6601 CGTTCACCGT TAGCTATTCT CACCGGCGGG AGGTGGGATC TTCTGCAGCC GAGTGTAGGT
     6661 ATATCCGCAA TGCACATGCA GCTTCTACAC AACAACAAAG TCGTCATCTT TGACCGTACG
     6721 GATTATGGAC CTTCGAATGT TTCTCTCCCT TCTCAAACCT GCCAAAACGC CACCGTTTTT
     6781 GACTGCTCTG CTCATTCAAT ACTATACGAC GTCGCTTCAA ATACCTTTCG CCCACTTACA
     6841 CTACGTTACG ACACGTGGTG CTCTTCCGGT TCTTTAAACG CTTCCGGTTC TTTAATCCAA
     6901 ACCGGCGGTT ATGGCAACGG CGAAAGAACT GTACGGGTTT TCACACCTTG CGACGGAGGA
     6961 GTTGGAAGCG TTTCGTGTGA TTGGATTGAG AACAGAGCGT ATCTCTCCTC TCGCCGGTGG
     7021 TATTCCACCA ATCAGATTCT TCCCGATGGT CGGATTATCA TCGTCGGCGG AAGAAGAGCT
     7081 TTCAACTACG AGTTTTACCC CAAGGATCCG GGAGAATCCG TTTTCAATCT CAGATTCTTG
     7141 GCGGAGACGA GAGATCCGAA TGAAGAAAAC AATCTTTATC CGTTTCTTCA TCTTCTCCCC
     7201 GACGGTAATC TCTTCATCTT CGCCAATCGG AGATCGATTT TGTTCGATTT CGTCAATCAC
     7261 CGAATCATCA AGGAGTTTCC ACAGATTCCC GGCGGCGATA AACGGAATTA TCCCAGCACT
     7321 GGCTCCTCCG TGCTCCTCCC TCTCTTTCTC ACCGGAGACA TTAACAGAAC GAAGATAACT
     7381 GCTGAGGTTA TGGTATGCGG CGGTGCTCCG CCGGGAGCGT TCTTCAAAGC GGCGAGGACG
     7441 ATTCCTAAAA TCTTCGTCGC GGGATCACGC ACGTGTGGGA GATTGAAAGT GACCGACCCG
     7501 GATCCGAAAT GGGTCATGGA GCAAATGCCA TCGCCACGTG TCATGTCTGA TATGTTACTG
     7561 TTACCAAACG GTGACGTTTT GATCATTAAC GGTGCTGCTA ACGGAACCGC TGGTTGGGAA
     7621 GACGCGACGA ACGCTGTTCT CAACCCGATT TTATACTTAC CCGAGGAGCC TGACCAGACC
     7681 CGGAGATTCG AAATTCTCAC CCCGACTCGA ATCCCTAGAA TGTACCATTC GGCGTCTCTT
     7741 CTTCTGTCAG ACGGCAGAGT CTTAGTCGGA GGAAGCAATC CTCATCGAAA TTACAATTTC
     7801 ACGGCGAGGC CGTACCCAAC GGAGCTCAGC CTCGAGGCTT ATCTCCCGCG TTACCTGGAT
     7861 CCACAGTACG CACGTGTGAG ACCGACGATC ATCACGGTGG AGCTCGCCGG GAATATGTTG
     7921 TACGGTCAGG CTTTTGCCGT AACGTTCGCG ATTCCGGCAT TCGGAATGTT CGACGGAGGG
     7981 GTATCGGTGA GGCTCGTGGC GCCGTCGTTC AGCACTCACT CAACGGCGAT GAACCAGAGG
     8041 TTGTTGGTTT TGCGCGTCAG GCGCGTGAGT CAGTTATCTG TTTTCGCTTA TAAAGCTGAC
     8101 GTGGACGGGC CTACTAACTC CTACGTGGCA CCACCTGGGT ATTATATGAT GTTCGTGGTT
     8161 CACCGTGGAA TTCCGAGTGT AGCTGTTTGG GTTAAGATTG Gaagcttgat atcgaattcc
     8221 tgcagcccgg gggatccact agttctagag CCTGGAGGTG GAGGTGGAGC TGTGAGCAAG
     8281 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
     8341 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
     8401 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
     8461 TTCGGCTACG GCCTGATGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
     8521 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
     8581 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
     8641 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
     8701 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
     8761 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
     8821 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC
     8881 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
     8941 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGGATCC TGCTGGTGCT
     9001 GCTGCGCCTC TAGCATAAgc ggccgcgatc gttcaaacat ttggcaataa agtttcttaa
     9061 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     9121 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt ttttgattag
     9181 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     9241 taaattatcg cgcgcggtgt catctatgtt actagatcga gctccagctt ttgttccctt
     9301 tagtgagggt taattccgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9361 tgttatccgc tcacaattcc acacaacata cgagccggaa ghcataaagt gtaaagcctg
     9421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9481 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9541 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9661 ggataacgca ggaaagaaca tgaaggcctt gacaggatat attggcgggt aaactaagtc
     9721 gctgtatgtg tttgtttgag atctcatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9781 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9841 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9901 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10261 ctgcgctctg ctgaagccag ttaccttcgg aagaagagtt ggtagctctt gatccggcaa
    10321 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10441 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10501 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgtgtaac attggtctag
    10561 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
    10621 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
    10681 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
    10741 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
    10801 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
    10861 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
    10921 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
    10981 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
    11041 atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc
    11101 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
    11161 gtttagtctg accatctcat ctgtaacaac attggcaacg ctacctttgc catgtttcag
    11221 aaacaactct ggcgcatcgg gcttcccata caatcggtag attgtcgcac ctgattgccc
    11281 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
    11341 cggccttgag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
    11401 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
    11461 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
    11521 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
    11581 aatcaccaac tggtccacct acaacaa   
//
