LOCUS       GH1001_LY34_pGre       11835 bp    DNA        circular     04-AUG-2021
DEFINITION  Agrobacterium binary vector with kanamycin-and
            bialophos/phosphinothricin-resistance genes.
ACCESSION   .
VERSION     .
KEYWORDS    pGreenII 0229
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.
  TITLE     pGreen: a versatile and flexible binary Ti vector for
            Agrobacterium-mediated plant transformation.
  JOURNAL   Plant Mol. Biol. 2000;42:819-32.
  PUBMED    10890530
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   pGreen website
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, 10 Feb, 2016 from SnapGene Viewer 3.0.3
            http://www.snapgene.com
COMMENT     This plasmid must be transformed into an Agrobacterium strain that
            also carries a vector such as pSoup.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      63..498
                     /note="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
                     /locus_tag="pSa ori"
                     /label="pSa ori"
                     /ApEinfo_label="pSa ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    633..655
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
                     /locus_tag="LB T-DNA repeat"
                     /label="LB T-DNA repeat"
                     /ApEinfo_label="LB T-DNA repeat"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      679..930
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
                     /locus_tag="NOS terminator"
                     /label="NOS terminator"
                     /ApEinfo_label="NOS terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(950..1501)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /note="BlpR"
                     /note="confers resistance to bialophos or
                     phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIQTSTVNFRTEPQEPQ
                     E
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLG
                     S
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVG
                     F WQLDFSLPVPPRPVLPVTEI"
                     /locus_tag="phosphinothricin acetyltransferase"
                     /label="phosphinothricin acetyltransferase"
                     /ApEinfo_label="phosphinothricin acetyltransferase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(1544..1721)
                     /note="NOS promoter"
                     /note="nopaline synthase promoter"
                     /locus_tag="NOS promoter"
                     /label="NOS promoter"
                     /ApEinfo_label="NOS promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1968..1984
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 fwd"
                     /label="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1991..2009
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(9525..9543)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(9564..9580)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    9588..9604
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
                     /label="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(9612..9643)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9918..9942
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
                     /locus_tag="RB T-DNA repeat"
                     /label="RB T-DNA repeat"
                     /ApEinfo_label="RB T-DNA repeat"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(10033..10621)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(10792..11607)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     G
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     K
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     A
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     I ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /locus_tag="aminoglycoside phosphotransferase"
                     /label="aminoglycoside phosphotransferase"
                     /ApEinfo_label="aminoglycoside phosphotransferase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9255..9506
                     /note="NOSt (clone first)"
                     /locus_tag="NOSt"
                     /label="NOSt"
                     /ApEinfo_label="NOSt"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8478..9246
                     /locus_tag="Citrine"
                     /label="Citrine"
                     /ApEinfo_label="Citrine"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2024..6501
                     /locus_tag="RUBYp with 5'UTR"
                     /label="RUBYp with 5'UTR"
                     /ApEinfo_label="RUBYp with 5'UTR"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6508..8427
                     /locus_tag="GOXL6"
                     /label="GOXL6"
                     /ApEinfo_label="GOXL6"
                     /ApEinfo_fwdcolor="#a5095a"
                     /ApEinfo_revcolor="#a5095a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc
      541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct
      601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta
      661 tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
      721 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
      781 tcaaaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
      841 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
      901 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat cagatttcgg
      961 tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca
     1021 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccacgg ggggcatatc
     1081 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc
     1141 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc
     1201 ccgtccgctg gtggcggggg gagacgtaca cggttgactc ggccgtccag tcgtaggcgt
     1261 tgcgtgcctt ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga
     1321 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct
     1381 gcggctcggt acggaagttg accgtgcttg tctggatgta gtggttgacg atggtgcaga
     1441 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca
     1501 tggtagatcc ccctcgatcg agttgagagt gaatatgaga ctctaattgg ataccgaggg
     1561 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac
     1621 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac
     1681 tccagaaacc cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa
     1741 acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctcatgtatc
     1801 gataacatta acgtttacaa tttcgcgcca ttcgccattc aggctgcgca actgttggga
     1861 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1921 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1981 cagtgaattg taatacgact cactataggg cgaattgggt accgctaacc caatctcaat
     2041 cgaaccggac caaattagaa tgaaatggtt ttagtgagtt acatggttct taatttgact
     2101 ggatgaaatt aattaaaggc actatataga tgaaactaaa actacaagaa cattgctcta
     2161 acttttacac aactttaaaa aaaaaaaaaa aaaaaatcgt ggaatgttga ttcatttgac
     2221 ctttattctg gattgaaagt gatgcaaaaa tgactttatc taataaataa aaaaaagaag
     2281 actttatctg atgtagtgaa attcttgtat ttgttgatgc aatgatgcta tggattgttt
     2341 tcaaatgttt caattacgta tcaaattatt ttatttgtac caacacatgc aagtatgcaa
     2401 ccatggcctc gtcgtaaaga actttgggca acatacaaat ttgggcaaac atctataaca
     2461 cattggattt ttttgatcga tttaatttga ttcttggaat tactattacc ttcttctttt
     2521 aaaaattcag taagatatgt gttaccgtgt gaaagtctat atgtgtggga acgaaaggtt
     2581 tgaagacgag taagaaagta gaagagcaca atgcgaaact ttattggtat acgtaactgt
     2641 ttattgacga catttgtagg aacaaaaatt gcaagataac acatttttta gtgtttgctt
     2701 tgcttgttta tagtaaccgg taggcagtgg cggaggcaga ataattagat acaggggtca
     2761 ctctttttgt aagccaaata ccaaaaaaaa aaaattaaga aaaatgataa tatcctcata
     2821 aatgataatt tcaaaaaaaa atatatatat gagaaagaaa aaaactgttc tagtaaagta
     2881 aacattaaat ctaaagtaat ttatactgtg ttaaacaata ataatgagat taacttagtt
     2941 taatagactt atttttttat ttataaaatt aattgggtta atttgttgta ggtttaatat
     3001 tttttgttag actatatttg aatatatatt gatgggggtc aataatattt tgggaaaggg
     3061 tcaatttata aaaattaaca aaacctaaag taattttcta aaatccatag gggtcaattg
     3121 acccccatga ttatacactc cctccgccac tgccggtagg gtttgatgtc ttggattgtt
     3181 aagttttaaa gttttgtctc atgatctatg taagtgctta agccggcgct agcataaatt
     3241 gaacctttaa tcagaatgta gtagttgacc tggatggatg acacttttat gcgagatggg
     3301 tcgagcgacg ctaaccatta ggccctcgat cggcagtcgg cagatataac ttagatacag
     3361 tggatagtga catatcctct taataaatat gttttaaatg ttagattaac ttatgaccta
     3421 aagaaaagaa gttagattaa cattttttag aaattattat ttcggatagg tttaataagc
     3481 ataagtcagc gaaacttata aggtatttat ttacaattat aaaatcaaca gcttccaaac
     3541 aaaatctttt ttctttttga cttcttaaaa caaaatctca tactaaccga tttaacatta
     3601 tgtttgtgaa taatatggta acgcgaatga ttagcaacat aaaaaacaaa gagtcaacta
     3661 accgcattca tgaatggaat ggaatgtcac ggtcaatcat tagatttggt gttacggaat
     3721 acatatttat taaatgtata tgaagaaaaa agcatggcca ataataatgt aaataaattg
     3781 gctcaacatg cgatgtatgt acataattta gagtggaaaa tgaaaagata tatacaatgt
     3841 gtattaatat agatgtacgc agttgtataa aattgatgga catgatttag gttttttttt
     3901 aaaaaattga tggacatttt tcaaatatca aaatgtttaa tttatatttt ctacatataa
     3961 ttgaaattaa acaaaggaga tataagttct acataatttt actttgtatc gttggaacaa
     4021 acacgtacag tagtattatt ttatatatgt gcatgtatgt tttgcgttcc tttaaaaatc
     4081 aaatacatgg gaagataaca tgcaacgaac cagatgatac agcgcctgat gagaggtcaa
     4141 aaacgtaacg agtttgactt gtataatatt aatatgtagt tgcatgtttg atgttgacac
     4201 agcatcgcca tcattaattc gttctctgca gtctaccgaa ctaattaatc tcaaataatc
     4261 gatgagtaca tatatgttaa gtccgtcgag gctaatgtat aattcaataa tataaatatg
     4321 ttcattcgag gatatagttc atattattat atatctgttt aaaattattg actccaatca
     4381 tcctataaac taagagggtt gacaccgcag caaagaaaaa aataaaatat accataattc
     4441 atatatttgc acaatctgaa ttataatgga aattaagttt gctcaaacaa aaaaaaaggg
     4501 ttggctccat atacgaaatt gttaatttgt aattttgtag gacttctaat taaaaaaata
     4561 tatatcaata aatgattatt ttatatatgt caattatatc tgatcattaa ttaattaatt
     4621 gtttcgaaaa taattaatta attttgaaat ttaaattaaa gcaatattaa atgtaaaaat
     4681 tttaaattat attaaactag aaatttattg tgagcatttt tgtgtataga tcaagatatg
     4741 atgaatcttg tgggattttt tttattctat aacaggtgtt gtttcatatg ctacgcattt
     4801 ttatatattt aaccaaacat aatgacaagt tgacaacgac tagtatttct tcccatttag
     4861 atgataagat tagtccaaat actccaaacc gtctgttgga ttaagaatga ccggaatacg
     4921 attactccaa aacgcatatg ggtttactgg tcgagaatat acctttggat taagatcatg
     4981 tagagttatt tcattacgat tttgatttct tttggttatt gtgaatatgt tttttacttt
     5041 cttttttctg ttagagatca tttgtgaata tgttaagtgg taagtgtaca atttttcata
     5101 ttttgaatcg agtatcataa atatcattgt acacatacca gtttatttat ttcgtcctga
     5161 tattaacaag taacaacgtc aattttgaga tcaattattt gaaaatatag aaaactgaaa
     5221 gtattaacca atttagtttg attctaatta gagtcatcgt attgtgctag taatcggtga
     5281 cgggaaccac ataaaacgac agtttatcaa cataaaaaaa aatcacaaac acatagaaga
     5341 ctctagtttt gttataaact tgtgctaaat aattaacacg atacataata ctcttcatta
     5401 ttatatatac ttttcatgta taataatgca tggacattac acaaatggaa tgaatagtac
     5461 aattgtatgt gcatgaatgt ttttctaaaa actgacatcc gtttgtatgc tattttatcc
     5521 ttgtttgtta tttaatagta gaaaacttgc gaggaccaaa ataattagca tgcaaagaac
     5581 cagaagaaat cgtgagaact aaaacgtaaa gaactcttgt gacttgtata atatatagtt
     5641 tgtatgcatg tttgatgttg acacaacata tctccatcat taattcgtcc cttgcagtct
     5701 ctcaaactta ttaatctcaa ataatcgatg tttatagatt aagtccctcg agactaataa
     5761 tgtaaatcca atgatacaat gtggttcctt cgacgacgaa tcatattctg atttctgtat
     5821 atctgtaata taaagtacga ttattattat atttgaatac aatcatactt aataaaatat
     5881 tgtgtcatat atatttatct ccataaatgt ttctcttgac tgaaatgtga atacccaaac
     5941 ataaaaacat atacagtaaa tactttgttc aattcccatt aaatttctct ttgaatctaa
     6001 aatcagacat agaaatactg ttgtttagag aaagattttg aatatataga acattaaatc
     6061 gccgcagaac tttgttgttt agagaaagat tttaaatatg ataagaactc aactaaagaa
     6121 aaagaaaaaa aattatgacg tctcaattta gagttttatt ggttcttttg tacagttctg
     6181 taatgttagg aatcgtttta tacacaaagg taacttaaat ctttcaaacg tctacggtag
     6241 tggacggtca ttaaaatcag agttaagaaa acgaacaaac caaatttaat catatcttat
     6301 acactcgaga ccttgattat atattactct tttatatatt aacagaacta gaaataaaaa
     6361 ctatacatta ttagaatatc gtaacgtata ctaatatccc atcgaccagt atataaacga
     6421 cacaagtcac agtacattct catcacatac cacaaaatat ctcataaaga tcaaataaac
     6481 actaaacctg aaagcaaaaa agggcccATG AAAGCCTCAA CAAGAGTCAT ATGGACAATA
     6541 TCTGTCCTTA TGCTCGCTGC TGTGTCCGAG GCCATCTTCC CTCTACCGTT TCTACCGTTC
     6601 CTCCCTGGGT TCAACAACGG CTTTAGGGAC AATGAGGCTG TCAAGGTTGC GAAACCACAG
     6661 CCTGCAGGAG CAGTTGGTGG AAAAGCCGCA AGAAGGCAGC GTGGCGGCGG TTTAGACGCT
     6721 CAGACAACCT GGGGTGGTAA GTGGGAGCTC TTCTTGGAAA ACTCGGGTGT GTCAGGGATG
     6781 CATGCGATCC TTATGCCAGT TATCAACAAG GTCCAGTACT ATGACGCCAC CATTTGGAGA
     6841 ATTTCAAAGA TTAAGTTGCC TCCAGGCGTA CCATGCCATG TCGTGGACGC TAAGACTAAC
     6901 AAGGTCGATT GTTGGGCTCA TTCGATTCTC ATGGATGTCA ACACCGGCGC CCTTAAGCCT
     6961 CTAGGGgtat gttcaaactc acgacaccat cttttttttt tttttttggg cctaggccta
     7021 gacacgggtt atcttcttct cattaaatag aatcatatta tacgaacttg aattaaatat
     7081 ttgcattcac atgtttcatg atagCTTTCC ACCGATACAT GGTGCTCATC CGGAGGTCTG
     7141 ACCGTAAATG GTACATTGGT GAGCACTGGA GGATACGGAG GAGGAGCCAA CACCGCGAGG
     7201 TACCTATCCA GCTGTGAAAA CTGCAAGTGG GAAGAATACC CTCAGGCATT GGCCGCGAAG
     7261 AGATGGTACT CAACGCAAGC AACTCTCCCT GACGGAAAGT TCTTCGTGAT TGGTGGAAGA
     7321 GACGCCTTGA ACTACGAATA CATTCCTGAG GAGGGACAAA ACAACAGGAA GCTCTTTGAC
     7381 TCATTGCTCC TCAGGCAAAC AGACGACCCG GAAGAAAACA ACCTTTACCC TTTCGTCTGG
     7441 CTTAACACCG ACGGTAACCT CTTCATTTTC GCTAACAACC GATCCATCCT TCTAAGCCCC
     7501 AAAACAAACC AAGTCATCAA GGAGTTCCCT CAGCTCCCAG GTGGTGCCCG TAACTACCCG
     7561 GGATCAGGTT CTTCCGCACT CCTCCCCATC CAACTTTACG TGAAAAACCC CAAGGTCATC
     7621 CCAGCCGAGG TTCTCGTCTG TGGTGGTTCC AAACAAGACG CGTATTACAA GGCTGGTAAA
     7681 AAAATATACG AACCAGCACT ACAAGACTGC GCCAGGATAA GGATCAACAG CGCAAAGCCA
     7741 CGATGGAAGA CGGAGATGAT GCCAACCCCA CGAATCATGA GTGATACTGT CATCCTCCCT
     7801 AACGGAGACA TCCTTCTAGT CAACGGAGCT AAACGTGGTT GTTCAGGCTG GGGATATGGT
     7861 AAAGACCCTG CCTTTGCTCC CCTCTTGTAC AAGCCCCATG CTGCACGTGG CAAGCGTTTC
     7921 AGACAGCTCA AGCCCACCAC CATCCCACGT ATGTACCACT CCAGCGCCAT CATTCTTCCA
     7981 GACGGTAAGG TTCTTGTCGG TGGCAGCAAC ACCAACGACG GTTACAAGTA CAACGTTGAG
     8041 TTCCCAACGG AGCTTCGCGT TGAGAAATTC TCCCCACCTT ACCTCGACCC GGCTCTAGCC
     8101 AACATAAGGC CAAAGATCGT CACCACCGGC ACCCCAAAAC AGGTCAAATA CGGACAATTC
     8161 TTCAACGTGA AGGTCGACCT TAAAGAAAAG GGAGCAACCA AGGGAAATCT CAAGGTTACC
     8221 ATGTTAGCCC CTGCCTTCAC AACACACAGT ATCTCAATGA ACATGAGGAT GCTCATCTTG
     8281 GGTGTCAATA ATGTCAAACC TGCTGGTGCC GGTTACGATA TCCAGGCCGT TGCACCACCA
     8341 AATGGAAACA TCGCTCCACC GGGTTACTAT CTTATCTTTG CCATCTACAA AGGTGTTCCC
     8401 AGCACTGGAG AATGGATTCA GGTCGTCGGa agcttgatat cgaattcctg cagcccgggg
     8461 gatccactag ttctagagCC TGGAGGTGGA GGTGGAGCTG TGAGCAAGGG CGAGGAGCTG
     8521 TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC
     8581 AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC
     8641 TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCTT CGGCTACGGC
     8701 CTGATGTGCT TCGCCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC
     8761 ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG
     8821 ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC
     8881 ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC
     8941 CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC
     9001 CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC
     9061 ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG
     9121 AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC
     9181 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGATCCTG CTGGTGCTGC TGCGCCTCTA
     9241 GCATAAgcgg ccgcgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     9301 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     9361 ttaacatgta atgcatgacg ttatttatga gatgggtttt ttgattagag tcccgcaatt
     9421 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     9481 cgcggtgtca tctatgttac tagatcgagc tccagctttt gttcccttta gtgagggtta
     9541 attccgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9601 acaattccac acaacatacg agccggaagh cataaagtgt aaagcctggg gtgcctaatg
     9661 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9721 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9781 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9841 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9901 aaagaacatg aaggccttga caggatatat tggcgggtaa actaagtcgc tgtatgtgtt
     9961 tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10021 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10081 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10141 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10201 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10261 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10321 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10381 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10441 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10501 gaagccagtt accttcggaa gaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10561 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10621 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10681 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10741 atgaagtttt aaatcaatct aaagtatata tgtgtaacat tggtctagtg attagaaaaa
    10801 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
    10861 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
    10921 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
    10981 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
    11041 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
    11101 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
    11161 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
    11221 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
    11281 tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt
    11341 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
    11401 catctcatct gtaacaacat tggcaacgct acctttgcca tgtttcagaa acaactctgg
    11461 cgcatcgggc ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg
    11521 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca
    11581 agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga
    11641 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
    11701 agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac
    11761 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg
    11821 gtccacctac aacaa    
//
