LOCUS       Exported                4086 bp DNA     circular SYN 20-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   gep
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2241..2353
                     /organism="Pseudoalteromonas translucida KMM 520"
                     /chromosome="I"
                     /strain="KMM 520"
                     /mol_type="genomic DNA"
                     /country="Japan"
                     /isolation_source="seawater"
                     /collection_date=1997
                     /db_xref="taxon:1315283"
                     /culture_collection="DSM:14402"
                     /type_material="type strain of Pseudoalteromonas
                     translucida"
     source          3042..3673
                     /organism="Pseudoalteromonas translucida KMM 520"
                     /chromosome="I"
                     /strain="KMM 520"
                     /mol_type="genomic DNA"
                     /country="Japan"
                     /isolation_source="seawater"
                     /collection_date=1997
                     /db_xref="taxon:1315283"
                     /culture_collection="DSM:14402"
                     /type_material="type strain of Pseudoalteromonas
                     translucida"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
     primer_bind     1844..1861
                     /label=Donor350F
     primer_bind     2206..2222
                     /label=M13 rev
     misc_feature    2241..2353
                     /label=RE
     misc_feature    2354..3041
                     /label=CmR
     misc_feature    3042..3673
                     /label=LE
     misc_feature    3680..3691
                     /label=MCS
     primer_bind     complement(3692..3708)
                     /label=M13 fwd
     primer_bind     complement(3925..3942)
                     /label=Donor-250R
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt ttgggtgttg tttgaagtat aagttgacat atctgtacta
     2281 aaagatggca taaattggaa gtgtaaggtg gcatagtcta gtatttaacc aaatggttaa
     2341 atggttgact cacatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     2401 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     2461 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     2521 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggagt tccgtatggc
     2581 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     2641 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     2701 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     2761 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     2821 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacta tgggcaaata
     2881 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     2941 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     3001 gggcggggcg taattttttt aaggcagtta ttggtgccct tttaattttc cttaattatt
     3061 tttaaagtta gactgattta gacttggaaa agcttaatga ttggagagct aaattgacta
     3121 atagtattca gtcaagttta attagtttta agcgatccat gcataactat ttctgtacaa
     3181 tgctatattt tcaccaatta ataattttta atcaagccta cattatgaaa tatactatac
     3241 ccatttgaac tcttctattt gtaccattgt cggtagcaaa aacttatggg gttttgaacg
     3301 tcacttaaat tgtaagcatt tgcgatggag gcgcgtttag agtcaacctt gattctgata
     3361 tgctccgaat ttttggtaag aatataagtg tgagagtagc taatgtggat acgcctgagt
     3421 taagggaaaa atgtgaaaat gaaataactc gttatcatgc aaagtgacta aggttataat
     3481 cttccgttta tggcacatag cagccaacta aacttgacag tatttttatg tggttggctt
     3541 tataaaacca gcatttggta acattatgcc aatttttact tcaatattat gccaacatac
     3601 actacactaa cggagctgta gcacaataag ctcgtttgta cttatgccaa cttatacttc
     3661 aaacaacatt gggggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact
     3721 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3781 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3841 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3901 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     3961 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4021 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4081 gcgcga
//
