LOCUS       pENTR/D-Topo_genomic_IMP1_(no_stop)        5574 bp    DNA     circular UNA 23-AUG-2021
DEFINITION  pENTR/D-Topo genomic IMP1 (no stop).
ACCESSION   urn.local...37-drp1wdf
VERSION     urn.local...37-drp1wdf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Terminator      complement(268..295)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="rrnB_T2 term"
                     /Transferred_Similarity="100.00%"
                     /label="rrnB_T2 term"
     Terminator      complement(427..470)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="rrnB_T1 term"
                     /Transferred_Similarity="100.00%"
                     /label="rrnB_T1 term"
     attL1           569..665
                     /label="attL1"
     gene            689..3682
                     /created_by="lwirthmu"
                     /modified_by="lwirthmu"
                     /label="genomic IMPORTIN ALPHA ISOFORM 1 (AT3G06720)"
     attL2           complement(3702..3798)
                     /label="attL2"
     Promoter        complement(3816..3834)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     Marker          3968..4777
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="kan2 marker"
                     /Transferred_Similarity="98.90%"
                     /label="kan2 marker"
     misc_feature    4908..5527
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat gtcactgaga cccaacgcta agacggaggt
      721 tcgccggaac cgatacaaag ttgcagtgga tgcggaggaa ggacgacgga ggagagaaga
      781 caacatggtt gagatccgta aaagcaagcg tgaggagagt ttgatgaaga agagacgtga
      841 aggtatgcaa gctcttcagg gtttcccttc agcttccgcc gcctccgtcg ataagaaggt
      901 acaattctgg tttttagatg attcgattgt actccttgtt gtcggttttc taggtttttg
      961 tttgtgattt tcatgtcggc ttactgaggt tttagggttt cgtttttggg ttctggttac
     1021 ggtagttgtc ttgtgattga ttggctttaa aaaaacattt gtagttggat agtctgaagg
     1081 atatggtcgc tggggtttgg tccgatgacc ctgccttgca gcttgagtcc actactcagt
     1141 tcaggaagct tctctctatt ggtaattcct tttcttaatt ctcttttatt tcccgttttg
     1201 atataatttg gagtgtcccc tttaactttt gtctctctgt ctctattata attgcataga
     1261 gagaagtcct ccaatcgagg aagtgataag tgctggtgtt gttcctaggt ttgttgagtt
     1321 tcttaagaaa gaagattacc ccgccattca ggtgagataa ttaatgtctc ttattgattc
     1381 attcttcact gtaatcttct cttctcatct tttatgattt ttattttagt ttgaggcagc
     1441 ttgggctcta acaaacattg catctggaac atcggatcac actaaggttg taatcgatca
     1501 caatgctgtt ccaatctttg ttcagcttct tgcttcccct agcgatgatg ttcgtgaaca
     1561 ggtatggaac ttctgatgac tacagaatgt cttctcattt attatgagag attatgcaat
     1621 tgaaaatttt gtgaaaccct gatcttaaga tttcgctcta acctgcaggc tgtatgggct
     1681 ttgggtaacg ttgctggtga ttcaccacgg tgccgtgatc ttgttcttgg atgtggggca
     1741 ctgcttccgc tgctcaatca gcttaatgag catgctaaat tgtccatgct tcgaaatgcc
     1801 acttggacct tgtcaaactt ctgtcgtggc aagcctcagc ctcactttga ccaggtctat
     1861 ttttttcccc tccaagcttg tttcgtcaat gtttatgtct agtattcttc taacatatga
     1921 agcatgttta acaggtcaaa cctgctcttc ccgcccttga acgactaatt cattcagatg
     1981 atgaagaagt cttgacagat gcctgttggg ctctttctta cctctctgat gggaccaatg
     2041 acaaaatcca gactgtcatc caggccggtg ttgtcccaaa acttgttgaa cttctcctgt
     2101 cagtaccatt ttataattcc cttatttcat gtccacttca cacatttgtt gacaaataac
     2161 ttgatacctt gctcttgatt tttagccatc attctccatc tgtgctaatt cctgctcttc
     2221 gcactgttgg aaatatagtt actggagatg atatacaaac acaggtatgg atctaaatcc
     2281 tgtctattga atttctcaca gtatttcggt tttgcacatt gtatatgata gtatatattt
     2341 ttcctactat gccaaagaat atctttgtgg ttatgcaatt ctttggcctt taggaatagg
     2401 tctgaagata ccactagaac ttttcattaa ttgaaccgat agcaaaaaca tgtcgaagtt
     2461 agtgatatac catgcatatc atctaatcca agactggtaa ttgcgatgag gtgttgttca
     2521 aatgtatggc accctgttgc tattattcat atccaacatt ttatgtgaaa aatgggagta
     2581 ttgaatgctt atcatgagat tctccctctg ttgccactct gtccatatta gcagatagga
     2641 tgcagtgcct tttttgctat atattgcttg tgtttgagtt ttgttacaat taattcttgt
     2701 tttaaagttt aaaccaacaa catgcacaaa ttttttgctt ggtgaaattt ggtttttcat
     2761 gagtttagca aaggtgattt ttctatatct gaaatagttt acatcgtttt acttggaaat
     2821 ctgattttac ttcttctcca gtgcgtgatc aatagtggtg ctctaccttg tcttgccaac
     2881 ctgctcactc aaaaccataa gaaaagcata aagaaggaag cttgctggac gatttcaaac
     2941 atcacagcag gcaacaaaga tcagatccag gtaggcaatt agtactgttt catcatatgt
     3001 tttgtaccgt ttcccatcca gacttctata cctattcatt aattgtgttg tttttcccct
     3061 gatggttgca gacggtagtt gaggctaatt tgatttcccc gttggtgagt ctgcttcaaa
     3121 atgctgaatt tgacattaag aaggaagctg catgggcaat ttcaaatgca acttcaggtg
     3181 gttctcatga tcaaataaag tatgtcactg tcttttttgc gttcctctaa cttccagttt
     3241 cttctctcat atatatactt tgtgtatata tatattgatg ttctgttccc gttggttata
     3301 cagatacctg gtggagcaag ggtgcataaa accattatgc gatctcctgg tttgcccaga
     3361 tccaagaatc ataactgtct gccttgaagg attggaaaac attttgaagg tgggagaggc
     3421 agagaagaac ttgggtcaca caggagacat gaactattac gctcagctga ttgatgatgc
     3481 agaagggcta gaaaagatcg agaaccttca gagccatgac aacaatgaga tctatgagaa
     3541 ggctgttaaa attctggaga catactggct tgaagaggaa gatgatgaga cacagcaacc
     3601 tccaggtgtt gatggttctc aggctgggtt ccagtttgga gggaatcaag ctccagtacc
     3661 atccggagga ttcaacttca gcaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     3721 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     3781 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     3841 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     3901 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     3961 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     4021 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     4081 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     4141 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     4201 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     4261 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     4321 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     4381 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     4441 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     4501 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     4561 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     4621 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     4681 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     4741 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     4801 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     4861 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4921 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4981 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5041 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5101 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5161 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5221 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5281 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     5341 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5401 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5461 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5521 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
