LOCUS       Exported                7981 bp ds-DNA     circular SYN 05-FEB-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 23, 2021 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    863..882
                     /label=T7
                     /note="color: #ffff00"
     misc_feature    895..900
                     /label=NheI
                     /note="color: #0080ff"
     misc_feature    901..2766
                     /label=DDX41
                     /note="color: #00ffff"
     misc_feature    1533..1552
                     /label=RTf primer
                     /note="color: #0080ff"
     misc_feature    1533..1552
                     /label=New Feature
                     /note="color: #ff0000"
     misc_feature    1611..1612
                     /label=Mutation
                     /note="color: #ffff00"
     misc_feature    1809..1828
                     /label=RTr primer
                     /note="color: #ff0000"
     misc_feature    2767..2772
                     /label=AgeI
                     /note="color: #80ff80"
     misc_feature    2812..3528
                     /label=EGFP
                     /note="color: #ff0000"
     misc_feature    2857..2878
                     /label=EGFP-N reverse primer
                     /note="color: #ffff00"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atggaggagt cggaacccga acggaagcgg gctcgcaccg acgaggtgcc tgccggagga
      961 agccgctccg aggcggaaga tgaggacgac gaggactacg tgccctatgt gccgttacgg
     1021 cagcgccggc agctactgct ccagaagctg ctgcagcgaa gacgcaaggg agctgcggag
     1081 gaagagcagc aggacagcgg tagtgaaccc cggggagatg aggacgacat cccgctaggc
     1141 cctcagtcca acgtcagcct cctggatcag caccagcacc ttaaagagaa ggctgaagcg
     1201 cgcaaagagt ctgccaagga gaagcagctg aaggaagaag agaagatcct ggagagtgtt
     1261 gccgagggcc gagcattgat gtcagtgaag gagatggcta agggcattac gtatgatgac
     1321 cccatcaaaa ccagctggac tccaccccgt tatgttctga gcatgtctga agagcgacat
     1381 gagcgcgtgc ggaagaaata ccacatcctg gtggagggag acggtatccc accacccatc
     1441 aagagcttca aggaaatgaa gtttcctgca gccatcctga gaggcctgaa gaagaaaggc
     1501 attcaccacc caacacccat tcagatccag ggcatcccca ccattctatc tggccgtgac
     1561 atgataggca tcgctttcac gggttcaggc aagacactgg tgttcacgtt gcccgtcatc
     1621 atgttctgcc tggaacaaga gaagaggtta cccttctcaa agcgcgaggg gccctatgga
     1681 ctcatcatct gcccctcgcg ggagctggcc cggcagaccc atggcatcct ggagtactac
     1741 tgccgcctgc tgcaggagga cagctcacca ctcctgcgct gcgccctctg cattgggggc
     1801 atgtccgtga aagagcagat ggagaccatc cgacacggtg tacacatgat ggtggccacc
     1861 ccggggcgcc tcatggattt gctgcagaag aagatggtca gcctagacat ctgtcgctac
     1921 ctggccctgg acgaggctga ccgcatgatc gacatgggct tcgagggtga catccgtacc
     1981 atcttctcct acttcaaggg ccagcgacag accctgctct tcagtgccac catgccgaag
     2041 aagattcaga actttgctaa gagtgccctt gtaaagcctg tgaccatcaa tgtggggcgc
     2101 gctggggctg ccagcctgga tgtcatccag gaggtagaat atgtgaagga ggaggccaag
     2161 atggtgtacc tgctcgagtg cctgcagaag acacccccgc ctgtactcat ctttgcagag
     2221 aagaaggcag acgtggacgc catccacgag tacctgctgc tcaagggggt tgaggccgta
     2281 gccatccatg ggggcaaaga ccaggaggaa cggactaagg ccatcgaggc attccgggag
     2341 ggcaagaagg atgtcctagt agccacagac gttgcctcca agggcctgga cttccctgcc
     2401 atccagcacg tcatcaatta tgacatgcca gaggagattg agaactatgt acaccggatt
     2461 ggccgcaccg ggcgctcggg aaacacaggc atcgccacta ccttcatcaa caaagcgtgt
     2521 gatgagtcag tgctgatgga cctcaaagcg ctgctgctag aagccaagca gaaggtgccg
     2581 cccgtgctgc aggtgctgca ttgcggggat gagtccatgc tggacattgg aggagagcgc
     2641 ggctgtgcct tctgcggggg cctgggtcat cggatcactg actgccccaa actcgaggct
     2701 atgcagacca agcaggtcag caacatcggt cgcaaggact acctggccca cagctccatg
     2761 gacttcaccg gttctggtgg gtcaggaggg ggcggaggta agcttggtac catggtgagc
     2821 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2881 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2941 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     3001 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     3061 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     3121 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     3181 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3241 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     3301 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3361 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3421 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3481 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagga attctaactc
     3541 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     3601 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3661 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     3721 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     3781 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     3841 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3901 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3961 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4021 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4081 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4141 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4201 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4261 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4321 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4441 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4501 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4561 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4621 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     4681 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4741 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4801 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4861 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4921 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4981 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5041 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5101 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5161 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5221 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5281 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5341 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5401 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5461 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5521 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5581 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5641 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5701 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5761 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     5821 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5881 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5941 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6001 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6061 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6121 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6181 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6241 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6301 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6361 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6421 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6481 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6541 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6601 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6661 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6721 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     6781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6901 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6961 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7081 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7141 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7261 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7861 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7921 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7981 c
//
