LOCUS       New_DNA                 7197 bp    DNA     circular     20-Nov-2015
DEFINITION  .
SOURCE      .
  ORGANISM  .
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label="T7promotor"
                     /note="; /ApEinfo_fwdcolor=#f3ffa3;
                     /ApEinfo_revcolor=#f3ffa3;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    19..41
                     /label="LacO"
                     /note="; /ApEinfo_fwdcolor=cornflower blue;
                     /ApEinfo_revcolor=cornflower blue;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             91..108
                     /label="6His"
     misc_feature    92..109
                     /label="HIS-tag"
                     /note="; /ApEinfo_fwdcolor=#fff81c;
                     /ApEinfo_revcolor=#fff81c;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    116..142
                     /label="Prescission site"
                     /note="; /ApEinfo_fwdcolor=#0bff9f;
                     /ApEinfo_revcolor=#0bff9f;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    116..139
                     /label="Presscission min"
                     /note="; /ApEinfo_fwdcolor=cyan; /ApEinfo_revcolor=green;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(154..>166,<2159..2171)
                     /label="USER_site"
                     /note="; /ApEinfo_fwdcolor=#f357ff;
                     /ApEinfo_revcolor=#f357ff;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0 [Split]"
     frag            167..2158
                     /date="17-Dec-2021"
                     /note="1 to 1992 of mmCKAP2_CCDS"
     CDS             167..2158
                     /label="mmCKAP2"
     CDS             827..868
                     /note="low complexity"
     CDS             1111..2119
                     /note="CKAP_C"
     misc_feature    2174..2881
                     /label="mNeonGreen"
                     /note="; /ApEinfo_fwdcolor=#19ff1c;
                     /ApEinfo_revcolor=#19ff1c;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2174..2881
                     /label="mNeonGreen"
     misc_feature    2900..2923
                     /label="Strep-tag"
                     /note="; /ApEinfo_fwdcolor=cyan; /ApEinfo_revcolor=green;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2900..2923
                     /label="StreptagII"
     primer_bind     complement(2940..2957)
                     /label="SP6"
                     /note="; /ApEinfo_fwdcolor=cyan; /ApEinfo_revcolor=green;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3029..3076
                     /label="b0016"
                     /note="; /ApEinfo_fwdcolor=#ff0000;
                     /ApEinfo_revcolor=#ff0000;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3106..3174
                     /label="LacZ alpha"
                     /note="; /ApEinfo_fwdcolor=cornflower blue;
                     /ApEinfo_revcolor=cornflower blue;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5087..5106
                     /label="pGEX 3'primer"
                     /note="; /ApEinfo_fwdcolor=#ffff00;
                     /ApEinfo_revcolor=#ffff00;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5535..6194
                     /label="AmpR"
                     /note="; /ApEinfo_fwdcolor=#f8c887;
                     /ApEinfo_revcolor=#f8c887;
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6292..6974
                     /label="ColE1 origin"
                     /note="; /ApEinfo_fwdcolor=gray50;
                     /ApEinfo_revcolor=gray50; /ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
BASE COUNT  1993 A  1756 C  1795 G  1653 T  0 OTHER
ORIGIN
        1 TAATACGACT CACTATAGGG AATTGTGAGC GGATAACAAT TCCTCTAGCA ATAATTTTGT
       61 TTAACTTTAA GAAGGAGATA TATCCATGAG TCATCACCAT CACCATCACT CCATGCTGGA
      121 AGTTCTGTTC CAGGGGCCCA CCATGGGTCG ACAGCTGAGG GGAGCTATGG CAGAGTCCAG
      181 GAAACGCTTC CTTGGCAGGG CTGCGAGGAA TCCCTTGCCG GTGACCCGGG ACCTGCAGCT
      241 GCCCCCGACC AGGCGAGACC AGCCGGCCTT CCGAGAACAA AGAAAACAAA AACTCAAGGA
      301 ATATCTGTTA ATACGAAAAA CAGTGTTTCC ATACAAACAA GAAAATCAGA TATCCAGAGA
      361 TCAGAAAATG ATAACATCTG AGGATCGGGT CCAAGAAGGG AAAAAAGTTG TGAAACTCAA
      421 AACAGAAGTG GCTGATAAAG AAAATATTGA AAGTACAGTT GAGAAAAACT GCATTCCATT
      481 AAAAGCTGGA GAAGTAACCA GCTCAGAAAT ACATAATTCA AAGGATAATG TTCAAGCTGT
      541 GCAGTTATTA TCTACAAGAG ATGATTTGCC AGGTCAGACT GTGACATTAG ACCCGGCATG
      601 TCACCATAAA GACAATAAAA AGATGCAAAT GACTGCAGAG AAGCCCAAAC AAGACAGTAA
      661 TGTGTCTAAA AAGCGAGTGC TTGGCTATTA TCATGGCCAG ATTGTTCAGT CTAAGATTAA
      721 TTCATTTAGA AAGCTTCCCA GTGTCAAAGG CGAGAGTCTT ACAACAACCA AGAAACTTCC
      781 CACTACTGTG TCTAAAGCCA TGAAGGCTCA GAGTGAGCCT GCGAACACCG TCAGCGTAAA
      841 AGCCTCAACT ACAGCTGCTG CCACCAAGTT TGCAGACGCT AAACCTGTGA GCACTGCGTC
      901 GAAGGACACA CTTGTGCGAC CACCTATCCG AAGCCTCCAC AGTAGTTCCC ACGGAGCTGC
      961 GAAACAGGGC CTCAGCAGGC CGCTTGCCAA TGTTACAGTC AGAAAAGGGA TGCTTGACAA
     1021 GGAGTCACAT CGATCAGAAC CAGTTGTGTC CGTTGTCAAA GCCGGTTCTT CTCAGGCCCC
     1081 ATCAAGAAGC ATAGCATCTA AAGATGCAGC CAGGACTGAT TCGTCTAATA CCAGACTGAT
     1141 GGTAAAGCCA AAAGATACTG ACCAGCGCAG ATACACGATT GCAGGAGCAA GTGTTCATAG
     1201 ATCAGCTCAG CTCAAGGACA CCACCGCAGA GAGGAAAGCT CAGATGACGG AGTGGAGAAC
     1261 TGGCAAAGGA AAGGGGCTAA AACGACCTCC TCATTCAGTA GTCACCCAGG CTGAGCCAAA
     1321 GGGACAAAGT GAAAACCCAG TTGGGTCCTT TTGGACTACC ATGGCAGAAG AAGATGAGCA
     1381 GAGATTATTT ACTGAAAAAG TAAACAAAAC TATTTCTGAA TGCCTGAACC TTATTAATGA
     1441 GGGATGCCCA AAAGAAGAAA TTTTGGCTAC ATTGAATGAC CTGATTCATA ATATTCCAGA
     1501 TGCCAAAAAA CTTGTTAAAT ATTGGATATG TCTTGTACGT ATTGAACCAA TCACAAGTCC
     1561 TATTGAAAAT ATTATCTCAA TCTATGAGAA GGCCATCCTG GCAGGGGCTC AGCCTATTGA
     1621 AGAGATGCGT CATATAATCA TAGATATTCT AACAACGAAG AGTCAAGAAA AAGTCAATTT
     1681 GGGAGAAAAT ATTGAGGAGG CTCATGCAAC CAAGGAACCT ATCCAAGAAG TAAATGCTGA
     1741 TGCTAATGTA GGGTCAGGAA AACCAGGAGA GGAGAATGAA CATCATGGCA AAGTTGAGGT
     1801 GTATGAAGAT GATCAAGACA ACAAAATAAA AGATCCGGAT TTAACAACTC CGGACTCAAA
     1861 AACTGAAGCA GGTTGCATAA TTCGATATAA TGTATCTTCT ACACCTCGGC TGCAAAGTAT
     1921 GAAAAAGATG CAACATGATA AAAATTCCAC ACTTAAAGAG CTAAAGTTCC TGACACCAGT
     1981 GAGACGCTCA CGGCGCATTC AAGACAAGAC TTCACGACTG CCAGCCATGT TAAAAGACCA
     2041 CGACCCTTCT GTGTCTTCCC TGGAGCAGTT GTCTGAGTTG GGAGGGGATG CCTTTGTCTG
     2101 CCGCCCTAAT GCAGCACTGT GCCCTCTGTT CTTTGAGACC GACGTAGCGG AAGAGGAAAG
     2161 TTCCCCTCAG CAAATGGTAT CCAAAGGTGA AGAGGACAAC ATGGCAAGCC TGCCGGCGAC
     2221 CCATGAGCTG CACATTTTTG GCTCCATTAA CGGCGTTGAT TTCGATATGG TCGGTCAGGG
     2281 TACTGGCAAT CCAAACGACG GCTACGAAGA ACTGAACCTG AAATCCACGA AAGGTGACCT
     2341 GCAATTTTCT CCTTGGATTC TGGTACCACA CATTGGCTAC GGTTTTCACC AATACCTGCC
     2401 GTATCCAGAT GGTATGAGCC CGTTTCAGGC GGCAATGGTG GATGGCTCTG GCTACCAGGT
     2461 TCATCGCACG ATGCAATTCG AGGACGGCGC GTCCTTAACG GTAAATTATC GTTATACTTA
     2521 TGAAGGCAGC CACATCAAAG GTGAAGCGCA GGTTAAAGGT ACTGGTTTTC CGGCCGATGG
     2581 TCCTGTTATG ACCAACAGCC TGACCGCCGC GGATTGGTGC CGCAGCAAAA AAACCTATCC
     2641 AAACGACAAA ACCATTATTT CTACGTTTAA ATGGTCGTAT ACTACGGGCA ATGGTAAACG
     2701 CTATCGTTCC ACGGCCCGCA CGACCTATAC GTTCGCAAAG CCAATGGCGG CCAACTACCT
     2761 GAAAAATCAG CCGATGTACG TGTTTCGCAA AACGGAACTG AAGCATTCTA AAACGGAACT
     2821 GAACTTTAAG GAATGGCAGA AAGCGTTTAC GGATGTGATG GGCATGGATG AATTGTACAA
     2881 AGGATCCGCG GCCGCGGCCT GGAGCCACCC GCAGTTCGAA AAATGAAAGC TTGAGTATTC
     2941 TATAGTGTCA CCTAAATCCC AGCTTGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA
     3001 GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT
     3061 CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TAACCTGGCG TAATAGCGAA
     3121 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGAAATTG
     3181 TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATATTTG TTAAATCAGC TCATTTTTTA
     3241 ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT
     3301 TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA
     3361 AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACA GCTTGCATGC CTGCAGGTCG
     3421 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT
     3481 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG
     3541 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT TCTTTTCACC
     3601 AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG
     3661 CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT TGATGGTGGT TGACGGCGGG
     3721 ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA CCGAGATATC CGCACCAACG
     3781 CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG CCATCTGATC GTTGGCAACC
     3841 AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA TGGTTTGTTG AAAACCGGAC
     3901 ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT
     3961 TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC
     4021 GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC CCAGTCGCGT ACCGTCTTCA
     4081 TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA CATCAAGAAA TAACGCCGGA
     4141 ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT CATCCAGCGG ATAGTTAATG
     4201 ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCGACG
     4261 CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA GTTGATCGGC GCGAGATTTA
     4321 ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC TGGAGGTGGC AACGCCAATC
     4381 AGCAACGACT GTTTGCCCGC CAGTTGTTGT GCCACGCGGT TGGGAATGTA ATTCAGCTCC
     4441 GCCATCGCCG CTTCCACTTT TTCCCGCGTT TTCGCAGAAA CGTGGCTGGC CTGGTTCACC
     4501 ACGCGGGAAA CGGTCTGATA AGAGACACCG GCATACTCTG CGACATCGTA TAACGTTACT
     4561 GGTTTCACAT TCACCACCCT GAATTGACTC TCTTCCGGGC GCTATCATGC CATACCGCGA
     4621 AAGGTTTTGC ACCATTCGAT GGTGTCGGGT ACCGAGCTCG AATTTACGTG AACCATCACC
     4681 CAAATCAAGT TTTTTGCGGT CGAGGTGCCG TAAAGCTCTA AATCGGAACC CTAAAGGGAG
     4741 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
     4801 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
     4861 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCG CCTGATGCGG TATTTTCTCC
     4921 TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA ATCTGCTCTG
     4981 ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG
     5041 CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT
     5101 GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC
     5161 TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC
     5221 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC
     5281 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA
     5341 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT
     5401 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG
     5461 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG
     5521 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TCATACACTA TTATCCCGTA
     5581 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GGGCGCGGTA TTCTCAGAAT GACTTGGTTG
     5641 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA
     5701 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG
     5761 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC
     5821 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG
     5881 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC
     5941 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG
     6001 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG
     6061 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA
     6121 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC
     6181 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA
     6241 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA
     6301 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG
     6361 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
     6421 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA
     6481 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC
     6541 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG
     6601 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC
     6661 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
     6721 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC
     6781 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
     6841 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC
     6901 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG
     6961 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT
     7021 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
     7081 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC
     7141 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGAT
//
