LOCUS       pCV329                  7073 bp ds-DNA     circular     03-JUL-2021
DEFINITION  .
KEYWORDS    "Key Features:YebS-YebT(P717N) expression plasmid, for             
            complementation." "Backbone:pET17b-yebst" "Construction:Gibson
            cloning into NdeI - XhoI-digested pBEL1620" "Resistance:Amp"
            "Location" "Rack" "Box" "Notes:pBEL1284 source of LEtA and LetB"
            "Sequencing information:y – 6-29-18 – pCV329-3 – CRVDN2479
            CRVDN2480 CRVDN2481 CRVDN2482 CRVDN2483 CRVDN2484  " "Special
            Category"
FEATURES             Location/Qualifiers
     primer          235..254
                     /label="oDCE3563"
                     /note="sequence: caagctgtgaccgtctccgg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(408..429)
                     /label="oDCE3648"
                     /note="sequence: catggcccgctttatcagaagc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          823..842
                     /label="oDCE3564"
                     /note="sequence: cacgttcgctcgcgtatcgg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     967..986
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1047..2330
                     /label="inner membrane PqiA domain protein"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1047..2330
                     /label="Translation 1047-2330"
     CDS             1047..2330
                     /label="Translation 1047-2330"
     CDS             2299..4932
                     /label="yebT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2299..4932
                     /label="Translation 2299-4932"
     misc_feature    2356..2418
                     /label="TM helix (TOPCONS)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2431..2709
                     /label="MCE domain (CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2632..2685
                     /label="pore_loop1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2773..3018
                     /label="MCE domain (weak/partial hit, CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2974..3021
                     /label="Pore loop ring 2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3133..3405
                     /label="MCE domain (CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3334..3381
                     /label="pore_loop_3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3469..3708
                     /label="MCE domain (weak/partial hit, CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3664..3735
                     /label="pore_loop4"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3835..4197
                     /label="Ring_5"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4030..4080
                     /label="pore_loop_5"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4195..4470
                     /label="MCE domain (CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4399..4446
                     /label="pore_loop_6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          4417..4473
                     /label="CVP823"
                     /note="sequence: ggcgttgagcatcttgatactatcctccagAATtatatcaacgtcgaaccaggccgg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(4418..4476)
                     /label="CVP822"
                     /note="sequence: gccccggcctggttcgacgttgatataATTctggaggatagtatcaagatgctcaacgc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4447..4449
                     /label="P717N"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4534..4791
                     /label="MCE domain (CDD)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4735..4803
                     /label="pore_loop_7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(4997..5015)
                     /label="T7 Term"
                     /note="sequence: GCTAGTTATTGCTCAGCGG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      5012..5058
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5461..5481
                     /label="oDCE3560"
                     /note="sequence: gagttttcgccccgaagaacg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             5492..6151
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          6080..6101
                     /label="oDCE3561"
                     /note="sequence: gggagtcaggcaactatggatg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      6249..6931
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          6695..6716
                     /label="oDCE3562"
                     /note="sequence: ctgagatacctacagcgtgagc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
       61 gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
      121 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
      181 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      241 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
      301 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
      361 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
      421 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
      481 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
      541 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
      601 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
      661 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
      721 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
      781 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
      841 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
      901 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat ctcgatcccg
      961 cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg
     1021 tttaacttta agaaggagat atacatatgg ctcttaacac accacaaatt acgccgacaa
     1081 aaaagataac agtgagggca atcggcgagg aactgccgcg tggtgattac caacgttgcc
     1141 cgcaatgtga catgctgttt agcctgcccg agataaattc tcatcaaagt gcctattgtc
     1201 cgcgctgtca ggcaaaaatt cgtgacgggc gcgactggtc gctaacgcgc ctggcggcaa
     1261 tggccttcac catgctgttg cttatgccgt ttgcctgggg cgaaccgctg ttgcatatct
     1321 ggctgttagg cattcgtatt gacgccaacg ttatgcaagg catctggcaa atgaccaaac
     1381 agggcgatgc gataacgggg tcgatggtct ttttctgcgt tattggtgcc ccgctcattc
     1441 tggtgacctc catagcttat ttatggtttg gtaaccgact gggaatgaat ttacgtccgg
     1501 tactgctgat gcttgagcga cttaaagagt gggtaatgct cgatatctac ctggtcggca
     1561 ttggcgttgc ttctataaag gtacaggatt atgcccatat ccaggctggt gtcggcttgt
     1621 tctcttttgt ggcgttggtg attttaacga cggtgacgtt gtcacatctt aatgtcgaag
     1681 aactgtggga gcgattttat ccgcagcgcc ccgctacgcg tagggacgag aaacttcgtg
     1741 tctgtcttgg gtgccatttt accggctatc cagatcagcg tggtcgctgc ccgcgttgcc
     1801 atatcccgct acgcctgcgt cgccgtcata gtctgcaaaa atgctgggcg gcgctgttag
     1861 cgtcaatcgt tttattgtta cctgccaacc tgttgcctat ttctatcatt tatctgaacg
     1921 gaggacggca ggaagataca attctttccg gaattatgtc gctggcaagt agcaacattg
     1981 cggttgcagg aatcgtgttt atcgccagta ttctggtacc gtttactaaa gtgatcgtca
     2041 tgttcacttt actgttgagc attcatttta aatgccagca aggtttacgc acacgcattc
     2101 tgttactgcg gatggtgacc tggattggtc gctggtcgat gctcgacctg tttgtcatat
     2161 ctttaaccat gtcgctgatt aatcgcgatc agatcctcgc ttttactatg ggaccggctg
     2221 cgttttattt cggcgcagcg gtaattttga ctattcttgc tgtggaatgg ctggacagcc
     2281 gcttactttg ggatgcacat gagtcaggaa acgcccgctt cgacgactga agcgcagatt
     2341 aaaaataaac gccgtatctc acctttctgg ctgctgccgt tcatcgcgct aatgattgcc
     2401 agttggctga tttgggacag ttatcaggac cggggtaata ccgtcaccat cgactttatg
     2461 tcggcggatg gtattgttcc aggccgtacg cctgttcgtt atcagggcgt tgaagtcgga
     2521 acagtgcagg atatcagcct cagcgacgat cttcgtaaga ttgaagtcaa ggtcagcatc
     2581 aagtccgata tgaaagatgc gctgcgcgaa gagactcagt tctggctggt gacgccaaaa
     2641 gcatcgttgg caggtgtctc cgggctggac gccctcgtcg gtgggaacta tatcggcatg
     2701 atgccgggta aaggtaaaga gcaggatcac tttgtcgcac tcgataccca accgaaatat
     2761 cggctggaca atggcgatct gatgatccac ctgcaagccc ccgatctcgg ttcgctgaac
     2821 agcggttcat tggtctattt ccgcaagatc ccggtgggaa aagtctacga ctatgccatc
     2881 aatcccaaca agcaaggcgt ggtgattgat gtcctgatcg agcggcgttt taccgatctg
     2941 gtgaaaaaag gtagccgttt ctggaacgtt tccggcgttg atgccaacgt cagtatcagt
     3001 ggcgcgaagg tgaaactgga aagcctggcg gcactggtta acggtgcgat tgccttcgat
     3061 tcaccagaag agtcgaaacc tgccgaggcg gaagatacct ttggtctgta tgaagatctg
     3121 gcccacagcc agcgtggcgt aataataaaa ctggaactgc cgagtggggc cggattaacc
     3181 gccgactcga cgccgttaat gtatcagggg ctggaagtcg gacagctgac taaactggat
     3241 ttaaatcctg gtggtaaagt cacaggggaa atgaccgttg atcccagcgt cgttaccctg
     3301 cttcgtgaaa atacccgcat cgaattacgc aacccgaaat tatcccttag cgatgctaat
     3361 ctcagcgccc tgctgaccgg caaaaccttc gagctggtgc ccggcgatgg cgagccacgc
     3421 aaagagttcg ttgttgtgcc aggcgaaaaa gcactgctgc atgaacctga tgttctgacg
     3481 ctgaccctga ccgcgccgga aagttacggt attgatgcgg gtcagccgct cattcttcac
     3541 ggcgtgcagg taggccaggt tatcgatcgt aaactcacca gcaaaggcgt cacctttacc
     3601 gtcgccatcg agcctcagca tcgcgaactg gtaaaaggcg atagcaaatt tgtcgtcaac
     3661 agccgtgtcg atgtgaaggt ggggctggat ggcgttgagt ttctcggtgc cagcgcctca
     3721 gaatggatca atggcgggat acgtattctg ccgggcgata aaggtgagat gaaagccagc
     3781 tatccactgt atgccaattt ggaaaaagcg ctggagaaca gccttagcga tttacccacc
     3841 acaaccgtga gtttgagtgc agagacgctg ccggatgtgc aggcaggatc ggtagtgctg
     3901 taccgtaaat ttgaagttgg tgaagtgatt accgtgcgtc cgcgagctaa cgcgtttgat
     3961 atcgatctgc atattaagcc ggagtatcgc aaccttctga ccagcaatag cgtgttctgg
     4021 gcagaaggcg gggcgaaagt tcagctgaat ggtagtggtc tgaccgtaca ggcatccccg
     4081 ctctccagag cattaaaggg agccattagc ttcgacaacc tcagcggtgc cagcgccagt
     4141 cagcgtaaag gcgataagcg tattctgtat gcttccgaaa cagcggcccg tgcggttggc
     4201 gggcagatta cgcttcacgc tttcgatgcc ggaaaactgg cggtcgggat gccaattcgc
     4261 tatctcggta ttgatatcgg gcaaatccag acgctggatc tgattaccgc acgcaatgaa
     4321 gtacaggcaa aggcggtact ttacccggaa tatgtccaga cctttgctcg cggtggtacg
     4381 cgcttctcag tggtcacacc gcaaatttcg gcagctggcg ttgagcatct tgatactatc
     4441 ctccagAATt atatcaacgt cgaaccaggc cggggcaatc ctcgccgcga ctttgaatta
     4501 caagaggcca ccattactga ttcgcgttac ctggatggct taagcattat tgttgaagcg
     4561 ccggaagccg gttcgttagg catcggtacg cctgtgctgt tccgtggtct ggaagtcggt
     4621 acggttacag gaatgacgct ggggacattg tcagatcgcg tgatgattgc gatgcgcatc
     4681 agtaaacgct atcaacacct ggtgcgtaac aattccgtct tctggttggc atcgggttac
     4741 agtctggact ttggtctgac gggcggcgta gtgaaaaccg gcacctttaa ccagtttatc
     4801 cgtggcggca tcgccttcgc cacgcctccg ggtacgccac tggcaccgaa agcccaggaa
     4861 ggcaaacact tcctgttgca ggaaagtgaa ccgaaagagt ggcgtgaatg gggaactgcg
     4921 cttcccaaat aaTGCCCACc tcgagcagat ccggctgcta acaaagcccg aaaggaagct
     4981 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     5041 gtcttgaggg gttttttgct gaaaggagga actatatccg gataattctt gaagacgaaa
     5101 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5161 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5221 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5281 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5341 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5401 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5461 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5521 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     5581 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5641 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5701 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5761 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5821 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5881 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5941 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6001 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6061 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6121 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6181 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6241 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6301 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6361 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6421 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6481 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6541 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6601 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6661 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6721 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6781 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6841 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6901 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6961 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7021 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agt
//