LOCUS       pAS139_pHTN_Halo-WIPI2B 7410 bp ds-DNA     circular     25-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1..741
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1067..1957
                     /label="HaloTag"
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_fwdcolor=#800040
     exon            1067..1957
                     /label="Translation 1067-1957"
     misc_feature    1958..1995
                     /label="HaloTag linker"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    1997..2014
                     /label="MCS"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    2021..3331
                     /label="WIPI2B"
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_fwdcolor=#0080c0
     misc_feature    2342..2344
                     /label="R108E"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    2687..2698
                     /label="FRRG PIP3 binding"
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_fwdcolor=#800080
     misc_feature    3332..3339
                     /label="MCS"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    5352..6212
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA
       61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
      121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
      181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
      241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
      301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
      361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA
      421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG
      481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC
      541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
      601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC
      661 CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC
      721 TGGTTTAGTG AACCGTCAGA TCACTAGAAG CTTTATTGCG GTAGTTTATC ACAGTTAAAT
      781 TGCTAACGCA GTCAGTGCTT CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC
      841 GTGAGGCACT GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA
      901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC
      961 TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA
     1021 AGGCTAGAGT ATTAATACGA CTCACTATAG GGCTAGCAAA GCCACCATGG CAGAAATCGG
     1081 TACTGGCTTT CCATTCGACC CCCATTATGT GGAAGTCCTG GGCGAGCGCA TGCACTACGT
     1141 CGATGTTGGT CCGCGCGATG GCACCCCTGT GCTGTTCCTG CACGGTAACC CGACCTCCTC
     1201 CTACGTGTGG CGCAACATCA TCCCGCATGT TGCACCGACC CATCGCTGCA TTGCTCCAGA
     1261 CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT TATTTCTTCG ACGACCACGT
     1321 CCGCTTCATG GATGCCTTCA TCGAAGCCCT GGGTCTGGAA GAGGTCGTCC TGGTCATTCA
     1381 CGACTGGGGC TCCGCTCTGG GTTTCCACTG GGCCAAGCGC AATCCAGAGC GCGTCAAAGG
     1441 TATTGCATTT ATGGAGTTCA TCCGCCCTAT CCCGACCTGG GACGAATGGC CAGAATTTGC
     1501 CCGCGAGACC TTCCAGGCCT TCCGCACCAC CGACGTCGGC CGCAAGCTGA TCATCGATCA
     1561 GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC CGCCCGCTGA CTGAAGTCGA
     1621 GATGGACCAT TACCGCGAGC CGTTCCTGAA TCCTGTTGAC CGCGAGCCAC TGTGGCGCTT
     1681 CCCAAACGAG CTGCCAATCG CCGGTGAGCC AGCGAACATC GTCGCGCTGG TCGAAGAATA
     1741 CATGGACTGG CTGCACCAGT CCCCTGTCCC GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT
     1801 TCTGATCCCA CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC CTGCCTAACT GCAAGGCTGT
     1861 GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC CCGGACCTGA TCGGCAGCGA
     1921 GATCGCGCGC TGGCTGTCGA CGCTCGAGAT TTCCGGCGAG CCAACCACTG AGGATCTGTA
     1981 CTTTCAGAGC GATAACGCGA TCGCTTCCGA ATTCggatcc atgaacctgg cgagccagag
     2041 cggggaggcc ggcgccggcc agctgctctt cgccaacttc aaccaggaca acacgtccct
     2101 agctgttggt agtaagtccg gttataaatt tttctccctt tcttctgtgg ataagctgga
     2161 acagatctat gaatgcaccg atacggaaga tgtgtgcatt gtagagagat tgttctccag
     2221 cagcctagtg gccatcgtga gccttaaagc accaaggaag ctaaaggttt gccactttaa
     2281 gaagggaact gagatctgca actacagcta ctccaacacg attctggctg tgaagctcaa
     2341 cgagcagagg ctgatagtat gcctggagga gtccctgtac atccacaaca ttcgggacat
     2401 gaaggtgctg catacgatca gggagacgcc tccaaaccct gcaggcctgt gtgcgctgtc
     2461 aatcaacaac gacaactgct acttggcgta cccagggagc gcgaccatcg gagaggtgca
     2521 ggtcttcgat accattaatt tgagagctgc aaacatgatt ccggctcacg acagtccttt
     2581 agcggcactg gcctttgacg caagtggaac taaacttgcc acggcttcgg agaaggggac
     2641 cgtgattagg gtattttcca ttccagaagg acaaaaactc tttgagtttc ggagaggagt
     2701 aaagaggtgc gtgagcatct gctccctggc cttcagcatg gacggcatgt tcctctccgc
     2761 ctccagcaac actgagaccg tgcacatctt caaactcgag actgtgaaag aaaaaccccc
     2821 agaggagccc accacctgga ccgggtactt cgggaaagtg ctcatggcct ccaccagcta
     2881 cctgccttcc caagtgacag aaatgttcaa ccagggcaga gccttcgcca cggtccgcct
     2941 gccattctgc ggccacaaaa acatctgctc gctagccaca attcagaaga tcccgcggtt
     3001 gttggtgggt gccgccgacg ggtacctgta catgtacaac ctggaccccc aggagggcgg
     3061 cgagtgtgcc ctgatgaagc agcaccggct ggacggcagt ctggaaacga ccaatgagat
     3121 cttggactct gcctctcacg actgcccctt agtcactcag acatacggcg cagctgcagg
     3181 aaaaggtact tacgtgcctt catccccaac gagacttgcc tacacagacg acctgggtgc
     3241 tgtgggtggc gcctgcctgg aggacgaggc cagcgccctg cgcctggatg aggacagcga
     3301 gcacccgccc atgattcttc ggactgactg aGCGGCCGCT TGCTGAGTTG GCTGCTGCCA
     3361 CCGCTGAGCA ATAACTAGCA TAACCCCTTG GCCGCTTCGA GCAGACATGA TAAGATACAT
     3421 TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT
     3481 TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA
     3541 CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGATG TGGGAGGTTT TTTTAAGCAA
     3601 GTAAAACCTC TACAAATGTG GTAAAATCGA ATTTTAACAA AATATTAACG CTTACAATTT
     3661 CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TACGCGGATC
     3721 TGCGCAGCAC CATGGCCTGA AATAACCTCT GAAAGAGGAA CTTGGTTAGG TACCTTCTGA
     3781 GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC
     3841 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG
     3901 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
     3961 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT
     4021 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT
     4081 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT
     4141 GATTCTTCTG ACACAACAGT CTCGAACTTA AGGCTAGAGC CACCATGATT GAACAAGATG
     4201 GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC
     4261 AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG
     4321 TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC
     4381 GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG
     4441 AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC
     4501 ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC
     4561 TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA
     4621 CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG
     4681 CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG
     4741 TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT
     4801 TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC
     4861 GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA
     4921 TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG
     4981 CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGATGGCC
     5041 GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GAATCGATAG
     5101 CGATAAGGAT CCTCTTTGCG CTTGCGTTTT CCCTTGTCCA GATAGCCCAG TAGCTGACAT
     5161 TCATCCGGGG TCAGCACCGT TTCTGCGGAC TGGCTTTCTA CGTAATGGTT TCTTAGACGT
     5221 CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC
     5281 ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA
     5341 AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT
     5401 TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC
     5461 AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA
     5521 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT CAAAGTTCTG CTATGTGGCG
     5581 CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC
     5641 AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG
     5701 TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC
     5761 TGACAACTAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG
     5821 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG
     5881 ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC
     5941 TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC
     6001 CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG
     6061 AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG
     6121 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG
     6181 AGATAGGTGC CTCACTGATT AAGCATTGGT AATTCGAAAT GACCGACCAA GCGACGCCCA
     6241 ACCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
     6301 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
     6361 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     6421 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     6481 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     6541 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     6601 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     6661 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     6721 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     6781 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA
     6841 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     6901 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATTTCAAGA
     6961 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     7021 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAT AGTCCGGAAA
     7081 TACAGGAACG CACGCTGGAT GGCCCTTCGC TGGGATGGTG AAACCATGAA AAATGGCAGC
     7141 TTCAGTGGAT TAAGTGGGGG TAATGTGGCC TGTACCCTCT GGTTGCATAG GTATTCATAC
     7201 GGTTAAAATT TATCAGGCGC GATTGCGGCA GTTTTTCGGG TGGTTTGTTG CCATTTTTAC
     7261 CTGTCTGCTG CCGTGATCGC GCTGAACGCG TTTTAGCGGT GCGTACAATT AAGGGATTAT
     7321 GGTAAATCCA CTTACTGTCT GCCCTCGTAG CCATCGAGAT AAACCGCAGT ACTCCGGCCA
     7381 CGATGCGTCC GGCGTAGAGG ATCGAGATCT
//