LOCUS       pCDH-EF1-tetR-iR        8068 bp    DNA     circular UNA 20-OCT-2021
DEFINITION  Ligation of  pCDH-mPGK-tetR-T2A-iRFP* into pCDH-EF1-tetR-mtaqBFP*.
ACCESSION   urn.local...i-dzyvpan
VERSION     urn.local...i-dzyvpan
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    7..234
                     /standard_name="RSV"
     misc_feature    235..414
                     /standard_name="5'LTR"
     misc_feature    1076..1308
                     /standard_name="RRE"
     gene            1798..1916
                     /standard_name="cPPT"
     gene            1928..2473
                     /standard_name="EF1"
     gene            2498..3130
                     /standard_name="tetR"
     gene            3146..3199
                     /standard_name="T2A peptide"
     gene            3206..4156
                     /standard_name="iRFP"
     gene            4163..4753
                     /standard_name="WPRE"
     gene            4825..5058
                     /standard_name="3'^LTR"
     misc_feature    5270..5416
                     /standard_name="SV40 ORI"
     gene            5786..6459
                     /standard_name="ori"
     misc_feature    complement(5786..6459)
                     /standard_name="pUC ORI"
     misc_feature    complement(6604..7464)
                     /standard_name="AmpR"
ORIGIN      
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gctagattag ataaaagtaa
     2521 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
     2581 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
     2641 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
     2701 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
     2761 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
     2821 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
     2881 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
     2941 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     3001 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     3061 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtggttcccc
     3121 aaaaaagaag ggatccgcgg ccgctgaggg cagaggaagt cttctaacat gcggtgacgt
     3181 ggaggagaat cccggcccta tgcatatggc ggaaggatcc gtcgccaggc agcctgacct
     3241 cttgacctgc gacgatgagc cgatccatat ccccggtgcc atccaaccgc atggactgct
     3301 gctcgccctc gccgccgaca tgacgatcgt tgccggcagc gacaaccttc ccgaactcac
     3361 cggactggcg atcggcgccc tgatcggccg ctctgcggcc gatgtcttcg actcggagac
     3421 gcacaaccgt ctgacgatcg ccttggccga gcccggggcg gccgtcggag caccgatcac
     3481 tgtcggcttc acgatgcgaa aggacgcagg cttcatcggc tcctggcatc gccatgatca
     3541 gctcatcttc ctcgagctcg agcctcccca gcgggacgtc gccgagccgc aggcgttctt
     3601 ccgccgcacc aacagcgcca tccgccgcct gcaggccgcc gaaaccttgg aaagcgcctg
     3661 cgccgccgcg gcgcaagagg tgcggaagat taccggcttc gatcgggtga tgatctatcg
     3721 cttcgcctcc gacttcagcg ggtccgtgat cgcagaggat cggtgcgccg aggtcgagtc
     3781 aaaactaggc ctgcactatc ctgcctcatt catcccggcg caggcccgtc ggctctatac
     3841 catcaacccg gtacggatca ttcccgatat caattatcgg ccggtgccgg tcaccccaga
     3901 cctcaatccg gtcaccgggc ggccgattga tcttagcttc gccatcctgc gcagcgtctc
     3961 gcccaaccat ctggagttca tgcgcaacat aggcatgcac ggcacgatgt cgatctcgat
     4021 tttgcgcggc gagcgactgt ggggattgat cgtttgccat caccgaacgc cgtactacgt
     4081 cgatctcgat ggccgccaag cctgcgagct agtcgcccag gttctggcct ggcagatcgg
     4141 cgtgatggaa gagtgagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     4201 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4261 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4321 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4381 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4441 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4501 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     4561 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4621 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4681 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4741 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4801 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc
     4861 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4921 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4981 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5041 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     5101 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     5161 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5221 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5281 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5341 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5401 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt
     5461 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     5521 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5581 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5641 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5701 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5761 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5821 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5881 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5941 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6001 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6061 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6121 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6181 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6241 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     6301 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6361 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6421 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6481 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6541 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6601 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6661 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6721 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6781 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6841 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6901 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6961 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7021 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7081 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7141 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7201 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7261 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7321 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7381 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7441 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7501 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7561 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     7621 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     7681 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     7741 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     7801 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     7861 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     7921 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     7981 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8041 ttgtaaaacg acggccagtg ccaagctg
//
