LOCUS       Exported                9791 bp ds-DNA     circular SYN 11-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   VB181207-1131dns
VERSION     .
KEYWORDS    pLV-Hygro-EF1A-FHA-Linker-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported to SnapGene on Dec 07, 2018
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 23, 2021 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
                     /note="/application_notes=Strong promoter; drives 
                     transcription of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=-Delta-5' LTR
                     /note="Delta-5' LTR"
                     /note="/application_notes=Allows transcription of viral RNA
                     and its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=-Psi
                     /label=nonstandard type: Miscellaneous
                     /note="Psi"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type: Miscellaneous
                     /note="RRE"
                     /note="/application_notes=Rev protein binding site that 
                     allows Rev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type: Miscellaneous
                     /note="cPPT"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1959..3137
                     /label=EF1A
                     /note="EF1A"
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor 1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type: Miscellaneous
                     /note="Kozak"
                     /note="/application_notes=Facilitates translation 
                     initiation of ATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    3180..3263
                     /note="FHA"
     misc_feature    3264..3311
                     /note="Linker"
     misc_feature    3312..4037
                     /label={Modified eGFP}
                     /label=nonstandard type: ORF
                     /note="{Modified eGFP}"
                     /note="/application_notes="
                     /note="/description=Plasmid designed to serve as template 
                     for building in components."
                     /note="/official_designation={Modified eGFP}"
                     /note="/uuid=ea968995ffc447e9b2c9cdfe6247fcf3"
     misc_feature    4076..4673
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="WPRE"
                     /note="/application_notes=Enhances virus stability in 
                     packaging cells, leading to higher titer of packaged virus;
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     promoter        4692..5202
                     /label=mPGK promoter
                     /note="mPGK promoter"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     misc_feature    5215..6240
                     /label=Hygro
                     /label=nonstandard type: Marker
                     /note="Hygro"
                     /note="/application_notes=Allows cells to be resistant to 
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             6311..6545
                     /label=-Delta-U3/3' LTR
                     /note="Delta-U3/3' LTR"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    6618..6752
                     /label=SV40 early pA
                     /note="SV40 early pA"
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     misc_feature    7706..8566
                     /label=Ampicillin
                     /label=nonstandard type: ORF
                     /note="Ampicillin"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      8737..9325
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC ori"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccacg cgtgccacca
     3181 tggcagttgt gattgggcgt ggcccagaga ccaagatcac tgataagaaa tgttctcgac
     3241 agcaagtaca gttgaaagca gagtctggtg gcggaggctc gggcggaggt ggatccggtg
     3301 gcggcggatc aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3361 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3421 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3481 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3541 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3601 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3661 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3721 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     3781 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     3841 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3901 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     3961 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4021 tgtacaagta atctagaacc cagctttctt gtacaaagtg gtgataatcg aattccgata
     4081 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4141 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4201 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4261 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4321 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4381 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4441 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4501 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4561 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4621 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcc
     4681 cgcggttcga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     4741 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4801 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4861 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4921 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     4981 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     5041 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     5101 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     5161 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctcacgtggc caccatgaaa
     5221 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     5281 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     5341 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     5401 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     5461 tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     5521 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     5581 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     5641 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     5701 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     5761 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     5821 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     5881 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     5941 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     6001 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     6061 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     6121 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     6181 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     6241 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6301 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     6361 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6421 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6481 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6541 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     6601 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6661 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6721 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     6781 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6841 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6901 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     6961 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     7021 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     7081 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     7141 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7201 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7261 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7321 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7381 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7441 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7501 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     7561 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7621 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7681 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7741 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7801 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7861 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7921 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7981 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8041 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8101 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8161 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8221 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8281 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8341 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8401 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8461 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8521 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8581 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8641 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8881 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagagaga aaggcggaca ggtatccggt
     9181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9421 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9481 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9541 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9601 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9661 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9721 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     9781 gctgcaagct t
//
