LOCUS       Exported                9962 bp ds-DNA     circular SYN 11-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   VB181207-1131dns
VERSION     .
KEYWORDS    pLV-Hygro-EF1A-WWE-Linker-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported to SnapGene on Dec 07, 2018
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 23, 2021 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
                     /note="/application_notes=Strong promoter; drives 
                     transcription of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=-Delta-5' LTR
                     /note="Delta-5' LTR"
                     /note="/application_notes=Allows transcription of viral RNA
                     and its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=-Psi
                     /label=nonstandard type: Miscellaneous
                     /note="Psi"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type: Miscellaneous
                     /note="RRE"
                     /note="/application_notes=Rev protein binding site that 
                     allows Rev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type: Miscellaneous
                     /note="cPPT"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1959..3137
                     /label=EF1A
                     /note="EF1A"
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor 1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type: Miscellaneous
                     /note="Kozak"
                     /note="/application_notes=Facilitates translation 
                     initiation of ATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    3168..3434
                     /note="WWE"
     misc_feature    3435..3482
                     /note="Linker"
     misc_feature    3483..4208
                     /label={Modified eGFP}
                     /label=nonstandard type: ORF
                     /note="{Modified eGFP}"
                     /note="/application_notes="
                     /note="/description=Plasmid designed to serve as template 
                     for building in components."
                     /note="/official_designation={Modified eGFP}"
                     /note="/uuid=ea968995ffc447e9b2c9cdfe6247fcf3"
     misc_feature    4247..4844
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="WPRE"
                     /note="/application_notes=Enhances virus stability in 
                     packaging cells, leading to higher titer of packaged virus;
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     promoter        4863..5373
                     /label=mPGK promoter
                     /note="mPGK promoter"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     misc_feature    5386..6411
                     /label=Hygro
                     /label=nonstandard type: Marker
                     /note="Hygro"
                     /note="/application_notes=Allows cells to be resistant to 
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             6482..6716
                     /label=-Delta-U3/3' LTR
                     /note="Delta-U3/3' LTR"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    6789..6923
                     /label=SV40 early pA
                     /note="SV40 early pA"
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     misc_feature    7877..8737
                     /label=Ampicillin
                     /label=nonstandard type: ORF
                     /note="Ampicillin"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      8908..9496
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC ori"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccacg cgtgccacca
     3181 tgatgggaaa tggtgaatat gcatggtatt atgaaggaag aaatgggtgg tggcagtacg
     3241 atgagcgcac tagtagagag ctggaagatg ctttttccaa aggtaaaaag aacactgaaa
     3301 tgttaattgc tggctttctg tatgtcgctg atcttgaaaa catggttcaa tataggagaa
     3361 atgaacatgg acgtcgcagg aagattaagc gagatataat agatatacca aagaagggag
     3421 tagctggact taggtctggt ggcggaggct cgggcggagg tggatccggt ggcggcggat
     3481 caatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3541 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3601 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3661 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     3721 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3781 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3841 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3901 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3961 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4021 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4081 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4141 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4201 aatctagaac ccagctttct tgtacaaagt ggtgataatc gaattccgat aatcaacctc
     4261 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4321 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4381 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4441 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4501 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4561 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4621 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     4681 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4741 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4801 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgggaattc ccgcggttcg
     4861 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     4921 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     4981 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     5041 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     5101 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     5161 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     5221 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     5281 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     5341 tctcctcttc ctcatctccg ggcctttcga cctcacgtgg ccaccatgaa aaagcctgaa
     5401 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     5461 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     5521 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     5581 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga atttagcgag
     5641 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     5701 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     5761 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     5821 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     5881 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     5941 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     6001 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     6061 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     6121 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     6181 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     6241 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     6301 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     6361 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gggtaccttt
     6421 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6481 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6541 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6601 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6661 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6721 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6781 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6841 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6901 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     6961 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7021 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7081 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     7141 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     7201 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     7261 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     7321 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7381 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7441 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7501 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7561 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7621 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7681 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7741 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7801 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7861 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7921 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7981 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8041 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8101 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8161 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8221 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8281 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8341 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8401 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8461 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8521 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8581 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8641 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8701 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8761 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8821 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     9061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9301 atgagaaagc gccacgcttc ccgaagagag aaaggcggac aggtatccgg taagcggcag
     9361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9541 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9601 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9661 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9721 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9781 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9841 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     9901 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     9961 tt
//
