LOCUS       Exported               10056 bp ds-DNA     circular SYN 11-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   VB190815-1112ufq
VERSION     .
KEYWORDS    pLV-EGFP-PolB(K35A:K68A:K72A)-PAMMut-Hygro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported to SnapGene on Oct 24, 2019
REFERENCE   2  (bases 1 to 10056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 23, 2021 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
                     /note="/application_notes=Strong promoter; drives 
                     transcription of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=-Delta-5' LTR
                     /note="Delta-5' LTR"
                     /note="/application_notes=Allows transcription of viral RNA
                     and its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=-Psi
                     /label=nonstandard type: Miscellaneous
                     /note="Psi"
                     /note="/application_notes=Allows packaging of viral DNA 
                     into virus."
                     /note="/description=Adenovirus packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type: Miscellaneous
                     /note="RRE"
                     /note="/application_notes=Rev protein binding site that 
                     allows Rev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type: Miscellaneous
                     /note="cPPT"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1959..2547
                     /label=CMV
                     /note="CMV"
                     /note="/application_notes=Strong promoter; may have 
                     variable strength in some cell types."
                     /note="/description=Human cytomegalovirus immediate early 
                     enhancer/promoter"
                     /note="/full_name=Human cytomegalovirus immediate early 
                     promoter"
                     /note="/official_designation=CMV"
                     /note="/uuid=bbb1016d51c4484eaa5a960df4edf21a"
     misc_feature    2572..2577
                     /label=Kozak
                     /label=nonstandard type: Miscellaneous
                     /note="Kozak"
                     /note="/application_notes=Facilitates translation 
                     initiation of ATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    2578..3294
                     /label=EGFP(ns)
                     /label=nonstandard type: ORF
                     /note="EGFP(ns)"
                     /note="/application_notes=Commonly used green fluorescent 
                     protein; ranked high in brightness, photostability and pH 
                     stability among all fluorescent proteins."
                     /note="/description=Enhanced green fluorescent protein; 
                     codon optimized based on a variant of wild type GFP from 
                     the jellyfish Aequorea victoria"
                     /note="/full_name=Enhanced green fluorescent protein(ns)"
                     /note="/official_designation=EGFP(ns)"
                     /note="/uuid=5443fd992fa248b69b274cf75346abad"
     misc_feature    3294..3295
                     /label=No linker
                     /label=nonstandard type: Linker
                     /note="No linker"
                     /note="/application_notes="
                     /note="/official_designation=No marker(ns)"
                     /note="/uuid=72e1edd2c1a540b28efbb477521f8696"
     misc_feature    3295..4302
                     /label={hPOLB(K35A/K68A/K72A)-PAM Mut}
                     /label=nonstandard type: ORF
                     /note="{hPOLB(K35A/K68A/K72A)-PAM Mut}"
                     /note="/application_notes="
                     /note="/official_designation=hPOLB[NM_002690.3]"
                     /note="/uuid=678fe9502d6c49079e0a902bde56376a"
     misc_feature    4341..4938
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="WPRE"
                     /note="/application_notes=Enhances virus stability in 
                     packaging cells, leading to higher titer of packaged virus;
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     promoter        4957..5467
                     /label=mPGK promoter
                     /note="mPGK promoter"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     misc_feature    5480..6505
                     /label=Hygro
                     /label=nonstandard type: ORF
                     /note="Hygro"
                     /note="/application_notes=Allows cells to be resistant to 
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             6576..6810
                     /label=3' LTR-Delta-U3
                     /note="3' LTR-Delta-U3"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    6883..7017
                     /label=SV40 early pA
                     /note="SV40 early pA"
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     misc_feature    7971..8831
                     /label=Ampicillin
                     /label=nonstandard type: ORF
                     /note="Ampicillin"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      9002..9590
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC ori"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2641 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2701 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2761 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     2821 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2881 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2941 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3001 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     3061 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     3121 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3181 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3241 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagatgagc
     3301 aaacggaagg cgccgcaaga gactctcaac gggggaatca ccgacatgct cacagaactc
     3361 gcaaactttg agaagaacgt gagccaagct atccacgcgt acaatgctta cagaaaagca
     3421 gcatctgtta tagcaaaata cccacacaaa ataaagagtg gagctgaagc taagaaattg
     3481 cctggagtag gaacagcgat tgctgaagcg attgatgagt ttttagcaac tggaaaatta
     3541 cgtaaactgg aaaagattcg gcaggatgat acgagttcat ccatcaattt cctgactcga
     3601 gttagtggca ttggtccatc tgctgcaagg aagtttgtag atgaaggaat taaaacacta
     3661 gaagatctca gaaaaaatga agataaattg aaccatcatc agcgaattgg gctgaaatat
     3721 tttggggact ttgaaaaaag aattcctcgt gaagagatgt tacaaatgca agatattgta
     3781 ctaaatgaag ttaaaaaagt ggattctgaa tacattgcta cagtctgtgg cagtttcaga
     3841 agaggtgcag agtccagtgg tgacatggat gttctcctga cccatcccag cttcacttca
     3901 gaatcaacca aacagccaaa actgttacat caggttgtgg agcagttaca aaaggttcat
     3961 tttatcacag ataccctgtc aaagggtgag acaaagttca tgggtgtttg ccagcttccc
     4021 agtaaaaatg atgaaaaaga atatccacac agaagaattg atatcaggtt gatacccaaa
     4081 gatcagtatt actgtggtgt tctctatttc actgggagtg atattttcaa taagaatatg
     4141 agggctcatg ccctagaaaa gggtttcaca atcaatgagt acaccatccg tcccttggga
     4201 gtcactggag ttgcaggaga acccctgcca gtggatagtg aaaaagacat ctttgattac
     4261 atccagtgga aataccggga acccaaggac cggagcgaat gaacccagct ttcttgtaca
     4321 aagtggtgat aatcgaattc cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4381 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4441 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4501 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4561 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4621 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4681 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     4741 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4801 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4861 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4921 gccgcctccc cgcatcggga attcccgcgg ttcgaattct accgggtagg ggaggcgctt
     4981 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     5041 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     5101 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     5161 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     5221 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     5281 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     5341 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     5401 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     5461 tcgacctcac gtggccacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     5521 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     5581 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     5641 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     5701 tccggaagtg cttgacattg gggaatttag cgagagcctg acctattgca tctcccgccg
     5761 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     5821 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     5881 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     5941 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     6001 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     6061 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     6121 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     6181 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     6241 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     6301 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     6361 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     6421 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     6481 cactcgtccg agggcaaagg aatagggtac ctttaagacc aatgacttac aaggcagctg
     6541 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6601 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6661 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6721 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6781 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6841 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7021 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7141 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7201 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7261 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7321 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7381 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7441 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7501 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7561 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7621 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7681 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7741 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7801 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7861 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8101 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8161 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8221 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8281 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8341 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8401 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8461 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8521 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8581 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8641 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8701 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8761 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8821 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8881 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9421 agagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9841 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9901 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     9961 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    10021 ctcactaaag ggaacaaaag ctggagctgc aagctt
//
