LOCUS       5768_SspB-HaloTag-DHPH- 6341 bp ds-DNA     circular     26-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    955..1845
                     /label="HaloTagV7 Cys Free"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1848..1854
                     /label="mCherry"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    604..936
                     /label="sspb Consensus"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2991..3005
                     /label="MCS"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     source          1848..2990
                     /label="Lck_mCherry_C1__"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             4237..5031
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PCR_primer      1386..1402
                     /label="f.halo_v7 seq.33.733"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PCR_primer      1562..1578
                     /label="f.halo_v7.33.876"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2977..2979
                     /label="END PH Joachim"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1876..2979
                     /label="mTiam1 (64-437)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    601..3024
                     /label="ORF"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     PCR_primer      1119..1139
                     /label="f.halo_v7 seq.33.732"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagCCACC
      601 ATGagctccc cgaaacgccc taagctgCTG CGTGAATATT ACGATTGGCT GGTTGATAAC
      661 AGCTTTACCC CATATCTGGT GGTGGATGCC ACATACCTGG GCGTGAACGT GCCCGTGGAG
      721 TATGTGAAAG ACGGTCAGAT CGTGCTGAAT CTGTCTGCAA GTGCGACCGG CAACCTGCAA
      781 CTGACAAATG ATTTTATCCA GTTCAACGCC CGCTTTAAGG GCGTGTCTCG TGAACTGTAT
      841 ATCCCGATGG GTGCCGCTCT GGCCATTTAC GCTCGCGAGA ACGGCGATGG TGTGATGTTC
      901 GAACCAGAAG AAATCTATGA CGAGCTGAAT ATTGGTAGTG GACCGGTCGC CACCatggcc
      961 gagatcggca ccggcttccc cttcgacccc cactacgtgg aggtgctggg cgagcgcatg
     1021 cactacgttg acgtgggccc ccgcgacggc acccccgtgc tgttcctgca cggcaacccc
     1081 accagcagct acgtgtggcg caacatcatc ccccacgtgg cccccaccca ccgcACTatc
     1141 gcccccgacc tgatcggcat gggcaagagc gacaagcccg acctgggcta cttcttcgac
     1201 gaccacgtgc gcttcatgga cgccttcatc gaggccctgg gcctggagga ggtggtgctg
     1261 gtgatccacg actggggcag cgccctgggc ttccactggg ccaagcgcaa ccccgagcgc
     1321 gtgaagggca tcgccttcat ggagttcatc cgccccatcc ccacctggga cgagtggccc
     1381 gagttcgccc gcgagacctt ccaggccttc cgcaccaccg acgtgggccg caagctgatc
     1441 atcgaccaga acgtgttcat cgagggcacc ctgcccatgg gcgtggtgcg ccccctgacc
     1501 gaggtggaga tggaccacta ccgcgagccc ttcctgaacc ccgtggaccg cgagcccctg
     1561 tggcgcttcc ccaacgagct gcccatcgcc ggcgagcccg ccaacatcgt ggccctggtg
     1621 gaggagtaca tggactggct gcaccagagc cccgtgccca agctgctgtt ctggggcacc
     1681 cccggcgtgc tgatcccccc cgccgaggcc gcccgcctgg ccaagagcct gcccaacGTT
     1741 aaggccgtgg acatcggccc cggcctgaac ctgctccagg aggacaaccc cgacctgatc
     1801 ggcagcgaga tcgcccgctg gctgagcacc ctggagatca gcggcGTgta caagtccgga
     1861 ctcagatcTC GAGaacagct gtcggatgcg gataagctgc gcaaggtgat ctgtgaactg
     1921 ctggagactg aacgcaccta tgtgaaagac ttaaactgcc tcatggagag atacctgaag
     1981 ccccttcaga aggagacctt tctcacccag gatgagcttg atgtactttt tggaaattta
     2041 accgaaatgg tggagtttca agtcgagttc cttaagactc tagaagatgg agtaagactg
     2101 gtccctgact tggaaaagct ggagaaggtt gaccagttca agaaagtgct cttctctctg
     2161 gggggatcct tcctgtacta cgcggaccgc ttcaagctct acagtgcctt ctgtgcgagc
     2221 cacacaaaag tccccaaggt cctggtgaaa gccaagacgg acacagcctt caaggcgttc
     2281 ctcgatgccc agaacccgag gcagcagcac tcatccacgc tggagtctta cctcatcaag
     2341 cccatccaga gggtcctcaa gtacccgctt ctgctcaggg agctgtttgc gctgaccgat
     2401 gcggagagcg aggaacacta ccacctggac gtggccatca agactatgaa caaggtcgcc
     2461 agtcacatca atgagatgca gaagatccac gaagagtttg gtgctgtgtt tgaccagctg
     2521 attgctgagc agacgggaga gaagaaagag gttgcagatc tgagcatggg tgacctgctc
     2581 ttgcacacca gcgtcatctg gctgaaccca cccgcctcac tgggaaagtg gaaaaaggag
     2641 ccagaattag cagccttcgt cttcaaaacg gccgtggtcc ttgtatataa agacggttcc
     2701 aagcagaaga agaaacttgt tggctctcac aggctgtcaa tctacgagga gtgggaccct
     2761 ttccggtttc gccacatgat ccctaccgaa gctttgcagg tccgagctct gcccagtgca
     2821 gatgcagagg caaatgcggt atgcgaaatt gtccacgtga aatcagagtc agaagggagg
     2881 cccgagcggg ttttccacct ctgctgcagt tccccagaga gcagaaagga ctttctgaag
     2941 tctgtgcatt cgatcctgcg agataaacac agaagacagG cgacggtacc gcgggcccgg
     3001 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     3061 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3121 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3241 atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     3301 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3361 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3421 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3481 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3541 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     3601 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3661 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     3721 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3781 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3841 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3901 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3961 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4021 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4201 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     4261 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4321 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4381 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     4441 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     4501 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     4561 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     4621 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     4681 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4741 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     4801 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4861 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4921 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     4981 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5041 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     5101 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5161 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     5221 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     5281 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     5341 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     5401 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     5461 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     5521 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5581 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5761 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     5821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6001 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6301 tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//