LOCUS       Exported               12641 bp ds-DNA     circular SYN 11-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   VB191022-1337pcf
VERSION     .
KEYWORDS    pLV-Hygro-EF1a-XRCC1-EGFP-T2A-myc-POLB(D256A:PAMmut)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported to SnapGene on Dec 12, 2019
REFERENCE   2  (bases 1 to 12641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 24, 2021 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12641
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
                     /note="/application_notes=Strong promoter; drives 
                     transcription of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="5' LTR-Delta-U3"
                     /note="/application_notes=Allows transcription of viral RNA
                     and its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=-Psi
                     /label=nonstandard type: Miscellaneous
                     /note="Psi"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type: Miscellaneous
                     /note="RRE"
                     /note="/application_notes=Rev protein binding site that 
                     allows Rev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type: Miscellaneous
                     /note="cPPT"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1959..3137
                     /label=EF1A
                     /note="EF1A"
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor 1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type: Miscellaneous
                     /note="Kozak"
                     /note="/application_notes=Facilitates translation 
                     initiation of ATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    3168..5783
                     /label={XRCC1-eGFP}
                     /label=nonstandard type: ORF
                     /note="{XRCC1-eGFP}"
                     /note="/application_notes="
                     /note="/official_designation={XRCC1-eGFP}"
                     /note="/uuid=9316b92aa566497e8e180d0d399d848b"
     misc_feature    5784..5846
                     /label=T2A
                     /label=nonstandard type: Linker
                     /note="T2A"
                     /note="/application_notes=Causes co-translational cleavage 
                     of the encoded polypeptide. Multiple proteins can be made 
                     from a polycistronic transcript containing multiple ORFs 
                     separated by 2A. P2A and T2A have higher cleavage 
                     efficiency compared to other 2As."
                     /note="/description=Self-cleaving 2A peptide from Thosea 
                     asigna virus"
                     /note="/full_name=Thosea asigna virus 2A peptide"
                     /note="/official_designation=T2A"
                     /note="/uuid=995274d0c39f4cbdbab66fb78aa938d6"
     misc_feature    5847..6887
                     /label={MYC-POLB-D256A(PAMmut)}
                     /label=nonstandard type: ORF
                     /note="{MYC-POLB-D256A(PAMmut)}"
                     /note="/application_notes="
                     /note="/official_designation={MYC-POLB-D256A(PAMmut)}"
                     /note="/uuid=d8cf6ebdb9e342ce8762c646de032431"
     misc_feature    6926..7523
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="WPRE"
                     /note="/application_notes=Enhances virus stability in 
                     packaging cells, leading to higher titer of packaged virus;
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     promoter        7542..8052
                     /label=mPGK promoter
                     /note="mPGK promoter"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     misc_feature    8065..9090
                     /label=Hygro
                     /label=nonstandard type: ORF
                     /note="Hygro"
                     /note="/application_notes=Allows cells to be resistant to 
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             9161..9395
                     /label=3' LTR-Delta-U3
                     /note="3' LTR-Delta-U3"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    9468..9602
                     /label=SV40 early pA
                     /note="SV40 early pA"
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     misc_feature    10556..11416
                     /label=Ampicillin
                     /label=nonstandard type: ORF
                     /note="Ampicillin"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      11587..12175
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC ori"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg ccggagatcc
     3181 gcctccgcca tgtcgtgtcc tgcagcagcc aggactcgac tcactgtgca gaaaatcttc
     3241 tcaaggcaga cacttaccga aaatggcggg cagccaaggc aggcgagaag accatctctg
     3301 tggtcctaca gttggagaag gaggagcaga tacacagtgt ggacattggg aatgatggct
     3361 cagctttcgt ggaggtgctg gtgggcagtt cagctggagg cgctggggag caagactatg
     3421 aggtccttct ggtcacctca tctttcatgt ccccttccga gagccgcagt ggctcaaacc
     3481 ccaaccgcgt tcgcatgttt gggcctgaca agctggtccg ggcagccgcc gagaagcgct
     3541 gggaccgggt caaaattgtt tgcagccagc cctacagcaa ggactccccc tttggcttga
     3601 gttttgtacg gtttcatagc cccccagaca aagatgaggc agaggccccg tcccagaagg
     3661 tgacagtgac caagcttggc cagttccgtg tgaaggagga ggatgagagc gccaactctc
     3721 tgaggccggg ggctctcttc ttcagccgga tcaacaagac atccccagtc acagccagcg
     3781 acccagcagg acctagctat gcagctgcta ccctccaggc ttctagtgct gcctcctcag
     3841 cctctccagt ctccagggcc ataggcagca cctccaagcc ccaggagtct cccaaaggga
     3901 agaggaagtt ggatttgaac caagaagaaa agaagacccc cagcaaacca ccagcccagc
     3961 tgtcgccatc tgttcccaag agacctaaat tgccagctcc aactcgtacc ccagccacag
     4021 ccccagtccc tgcccgagca cagggggcag tgacaggcaa accccgagga gaaggcaccg
     4081 agcccagacg accccgagct ggcccagagg agctggggaa gatccttcag ggtgtggtag
     4141 tggtgctgag tggcttccag aaccccttcc gctccgagct gcgagataag gccctagagc
     4201 ttggggccaa gtatcggcca gactggaccc gggacagcac gcacctcatc tgtgcctttg
     4261 ccaacacccc caagtacagc caggtcctag gcctgggagg ccgcatcgtg cgtaaggagt
     4321 gggtgctgga ctgtcaccgc atgcgtcggc ggctgccctc ccagaggtac ctcatggcag
     4381 ggccaggttc cagcagtgag gaggatgagg cctctcacag cggtggcagc ggagatgaag
     4441 cccccaagct tcctcagaag caaccccaga ccaaaaccaa gcccactcag gcagctggac
     4501 ccagctcacc ccagaagccc ccaacccctg aagagaccaa agcagcctca ccagtgctcc
     4561 aggaagatat agacattgag ggggtacagt cagaaggaca ggacaatggg gcggaagatt
     4621 ctggggacac agaggatgag ctgaggaggg tggcagagca gaaggaacac agactgcccc
     4681 ctggccagga ggagaatggg gaagacccgt atgcaggctc cacggatgag aacacggaca
     4741 gtgaggaaca ccaggagcct cctgatctgc cagtccctga gctcccagat ttcttccagg
     4801 gcaagcactt ctttctttac ggggagttcc ctggggacga gcggcggaaa ctcatccgat
     4861 acgtcacagc cttcaatggg gagctcgagg actatatgag tgaccgggtt cagtttgtga
     4921 tcacagcaca ggaatgggat cccagctttg aggaggccct gatggacaac ccctccctgg
     4981 cattcgttcg tccccgatgg atctacagtt gcaatgagaa gcagaagtta cttcctcacc
     5041 agctctatgg ggtggtgccg caagccatgg tgagcaaggg cgaggagctg ttcaccgggg
     5101 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     5161 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     5221 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     5281 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     5341 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     5401 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     5461 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     5521 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     5581 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5641 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     5701 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5761 tcggcatgga cgagctgtac aagggaagcg gagagggcag gggaagtctt ctaacatgcg
     5821 gggacgtgga ggaaaatccc ggccccatgg aacaaaaact catctcagaa gaggatctga
     5881 tgagcaaacg gaaggcgccg caagagactc tcaacggggg aatcaccgac atgctcacag
     5941 aactcgcaaa ctttgagaag aacgtgagcc aagctatcca caagtacaat gcttacagaa
     6001 aagcagcatc tgttatagca aaatacccac acaaaataaa gagtggagct gaagctaaga
     6061 aattgcctgg agtaggaaca aaaattgctg aaaagattga tgagttttta gcaactggaa
     6121 aattacgtaa actggaaaag attcggcagg atgatacgag ttcatccatc aatttcctga
     6181 ctcgagttag tggcattggt ccatctgctg caaggaagtt tgtagatgaa ggaattaaaa
     6241 cactagaaga tctcagaaaa aatgaagata aattgaacca tcatcagcga attgggctga
     6301 aatattttgg ggactttgaa aaaagaattc ctcgtgaaga gatgttacaa atgcaagata
     6361 ttgtactaaa tgaagttaaa aaagtggatt ctgaatacat tgctacagtc tgtggcagtt
     6421 tcagaagagg tgcagagtcc agtggtgaca tggatgttct cctgacccat cccagcttca
     6481 cttcagaatc aaccaaacag ccaaaactgt tacatcaggt tgtggagcag ttacaaaagg
     6541 ttcattttat cacagatacc ctgtcaaagg gtgagacaaa gttcatgggt gtttgccagc
     6601 ttcccagtaa aaatgatgaa aaagaatatc cacacagaag aattgctatc aggttgatac
     6661 ccaaagatca gtattactgt ggtgttctct atttcactgg gagtgatatt ttcaataaga
     6721 atatgagggc tcatgcccta gaaaagggtt tcacaatcaa tgagtacacc atccgtccct
     6781 tgggagtcac tggagttgca ggagaacccc tgccagtgga tagtgaaaaa gacatctttg
     6841 attacatcca gtggaaatac cgggaaccca aggaccggag cgaatgaacc cagctttctt
     6901 gtacaaagtg gtgataatcg aattccgata atcaacctct ggattacaaa atttgtgaaa
     6961 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7021 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7081 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7141 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7201 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7261 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7321 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7381 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7441 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7501 tttgggccgc ctccccgcat cgggaattcc cgcggttcga attctaccgg gtaggggagg
     7561 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     7621 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     7681 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     7741 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     7801 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     7861 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     7921 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     7981 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     8041 gcctttcgac ctcacgtggc caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     8101 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     8161 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     8221 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     8281 ccgattccgg aagtgcttga cattggggaa tttagcgaga gcctgaccta ttgcatctcc
     8341 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     8401 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     8461 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     8521 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     8581 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     8641 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     8701 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     8761 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     8821 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     8881 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     8941 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     9001 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     9061 cccagcactc gtccgagggc aaaggaatag ggtaccttta agaccaatga cttacaaggc
     9121 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     9181 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9241 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9301 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9361 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9421 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     9481 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9541 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     9601 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     9781 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     9841 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     9901 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     9961 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    10021 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    10081 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    10141 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    10201 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    10261 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    10321 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    10381 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
    10441 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10501 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    10561 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    10621 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10681 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10741 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    10801 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    10861 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    10921 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    10981 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    11041 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    11101 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    11161 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    11221 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    11281 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    11341 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    11401 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    11461 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    11521 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    11581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    11641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    11701 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    11761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    11821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    11881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    11941 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    12001 cgaagagaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    12061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    12121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    12181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    12241 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    12301 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    12361 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    12421 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    12481 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    12541 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
    12601 taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
