LOCUS       Exported               11774 bp ds-DNA     circular SYN 24-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   VB200710-1144mbw
VERSION     .
KEYWORDS    pLV-Hygro-EF1a-EGFP-PolB-T2A-myc-SIRT6(PAMmut-R65A)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 24, 2021 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11774
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
                     /note="/application_notes=Strong promoter; drives 
                     transcription of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="5' LTR-Delta-U3"
                     /note="/application_notes=Allows transcription of viral RNA
                     and its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=-Psi
                     /label=nonstandard type: Miscellaneous
                     /note="Psi"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type: Miscellaneous
                     /note="RRE"
                     /note="/application_notes=Rev protein binding site that 
                     allows Rev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type: Miscellaneous
                     /note="cPPT"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1959..3137
                     /label=EF1A
                     /note="EF1A"
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor 1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type: Miscellaneous
                     /note="Kozak"
                     /note="/application_notes=Facilitates translation 
                     initiation of ATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    3168..4889
                     /label=EGFP/hPOLB[NM_002690.3](ns)
                     /label=nonstandard type: ORF
                     /note="EGFP/hPOLB[NM_002690.3](ns)"
                     /note="/application_notes="
                     /note="/official_designation=hPOLB[NM_002690.3]"
                     /note="/uuid=a68e42af4c564821912e073a459bc2aa"
     misc_feature    4890..4952
                     /label=T2A
                     /label=nonstandard type: Linker
                     /note="T2A"
                     /note="/application_notes=Causes co-translational cleavage 
                     of the encoded polypeptide. Multiple proteins can be made 
                     from a polycistronic transcript containing multiple ORFs 
                     separated by 2A. P2A and T2A have higher cleavage 
                     efficiency compared to other 2As."
                     /note="/description=Self-cleaving 2A peptide from Thosea 
                     asigna virus"
                     /note="/full_name=Thosea asigna virus 2A peptide"
                     /note="/official_designation=T2A"
                     /note="/uuid=995274d0c39f4cbdbab66fb78aa938d6"
     misc_feature    4953..6020
                     /label={hSIRT6[NM_016539.4]PAMmut-R65A}
                     /label=nonstandard type: ORF
                     /note="{hSIRT6[NM_016539.4]PAMmut-R65A}"
                     /note="/application_notes="
                     /note="/official_designation=hSIRT6[NM_016539.4]"
                     /note="/uuid=a31261606337458a881952876da2c285"
     misc_feature    6059..6656
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="WPRE"
                     /note="/application_notes=Enhances virus stability in 
                     packaging cells, leading to higher titer of packaged virus;
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     promoter        6675..7185
                     /label=mPGK promoter
                     /note="mPGK promoter"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     misc_feature    7198..8223
                     /label=Hygro
                     /label=nonstandard type: ORF
                     /note="Hygro"
                     /note="/application_notes=Allows cells to be resistant to 
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             8294..8528
                     /label=3' LTR-Delta-U3
                     /note="3' LTR-Delta-U3"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    8601..8735
                     /label=SV40 early pA
                     /note="SV40 early pA"
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     misc_feature    9689..10549
                     /label=AmpiciIIin
                     /label=nonstandard type: ORF
                     /note="AmpiciIIin"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      10720..11308
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC ori"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gtgagcaagg
     3181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3301 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3361 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3601 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3661 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3781 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3841 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagatgagc aaacggaagg
     3901 cgccgcagga gactctcaac gggggaatca ccgacatgct cacagaactc gcaaactttg
     3961 agaagaacgt gagccaagct atccacaagt acaatgctta cagaaaagca gcatctgtta
     4021 tagcaaaata cccacacaaa ataaagagtg gagctgaagc taagaaattg cctggagtag
     4081 gaacaaaaat tgctgaaaag attgatgagt ttttagcaac tggaaaatta cgtaaactgg
     4141 aaaagattcg gcaggatgat acgagttcat ccatcaattt cctgactcga gttagtggca
     4201 ttggtccatc tgctgcaagg aagtttgtag atgaaggaat taaaacacta gaagatctca
     4261 gaaaaaatga agataaattg aaccatcatc agcgaattgg gctgaaatat tttggggact
     4321 ttgaaaaaag aattcctcgt gaagagatgt tacaaatgca agatattgta ctaaatgaag
     4381 ttaaaaaagt ggattctgaa tacattgcta cagtctgtgg cagtttcaga agaggtgcag
     4441 agtccagtgg tgacatggat gttctcctga cccatcccag cttcacttca gaatcaacca
     4501 aacagccaaa actgttacat caggttgtgg agcagttaca aaaggttcat tttatcacag
     4561 ataccctgtc aaagggtgag acaaagttca tgggtgtttg ccagcttccc agtaaaaatg
     4621 atgaaaaaga atatccacac agaagaattg atatcaggtt gatacccaaa gatcagtatt
     4681 actgtggtgt tctctatttc actgggagtg atattttcaa taagaatatg agggctcatg
     4741 ccctagaaaa gggtttcaca atcaatgagt acaccatccg tcccttggga gtcactggag
     4801 ttgcaggaga acccctgcca gtggatagtg aaaaagacat ctttgattac atccagtgga
     4861 aataccggga acccaaggac cggagcgaag gaagcggaga gggcagggga agtcttctaa
     4921 catgcgggga cgtggaggaa aatcccggcc ccatgtcggt gaattacgca gcagggctgt
     4981 cgccgtacgc ggacaagggc aagtgcggcc tcccggagat cttcgacccc ccggaggagc
     5041 tggagcggaa ggtgtgggaa ctggcgaggc tggtctggca gtcttccagt gtggtgttcc
     5101 acacgggtgc cggcatcagc actgcctctg gcatccccga cttcgcgggt ccccacggag
     5161 tctggaccat ggaggagcga ggtctggccc ccaagttcga caccaccttt gagagcgcgc
     5221 ggcccacgca gacccacatg gcgctggtgc agctggagcg cgtgggcctc ctccgcttcc
     5281 tggtcagcca gaacgtggac gggctccatg tgcgctcagg cttccccagg gacaaactgg
     5341 cagagctcca cgggaacatg tttgtggaag aatgtgccaa gtgtaagacg cagtacgtcc
     5401 gagacacagt cgtgggcacc atgggcctga aggccacggg ccggctctgc accgtggcta
     5461 aggcaagggg gctgcgagcc tgcaggggag agctgaggga caccatccta gactgggagg
     5521 actccctgcc cgaccgggac ctggcactcg ccgatgaggc cagcaggaac gccgacctgt
     5581 ccatcacgct gggtacatcg ctgcagatcc ggcccagcgg gaacctgccg ctggctacca
     5641 agcgccgggg aggccgcctg gtcatcgtca acctgcagcc caccaagcac gaccgccatg
     5701 ctgacctccg catccatggc tacgttgacg aggtcatgac ccggctcatg aagcacctgg
     5761 ggctggagat ccccgcctgg gacggccccc gtgtgctgga gagggcgctg ccacccctgc
     5821 cccgcccgcc cacccccaag ctggagccca aggaggaatc tcccacccgg atcaacggct
     5881 ctatccccgc cggccccaag caggagccct gcgcccagca caacggctca gagcccgcca
     5941 gccccaaacg ggagcggccc accagccctg ccccccacag accccccaaa agggtgaagg
     6001 ccaaggcggt ccccagctga acccagcttt cttgtacaaa gtggtgataa tcgaattccg
     6061 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6121 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6181 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6241 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6301 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6361 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6421 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     6481 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6541 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6601 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgggaat
     6661 tcccgcggtt cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     6721 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     6781 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     6841 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     6901 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     6961 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     7021 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     7081 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     7141 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctcacgt ggccaccatg
     7201 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     7261 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     7321 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     7381 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     7441 gaatttagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     7501 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     7561 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     7621 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     7681 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     7741 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     7801 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     7861 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     7921 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     7981 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     8041 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     8101 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     8161 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     8221 tagggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8281 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     8341 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8401 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8461 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8521 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8581 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     8641 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8701 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     8761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8821 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8881 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     8941 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     9001 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     9061 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     9121 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9181 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9241 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9301 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9361 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9421 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9481 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     9541 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    10021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    10081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    10141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    10201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    10261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    10321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10861 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    11041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    11101 acagcgtgag ctatgagaaa gcgccacgct tcccgaagag agaaaggcgg acaggtatcc
    11161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    11221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    11281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    11341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    11401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    11461 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    11521 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    11581 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11641 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11701 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
    11761 ggagctgcaa gctt
//
