LOCUS       Exported               12683 bp ds-DNA     circular SYN 11-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   VB191212-2224xsf
VERSION     .
KEYWORDS    pLV-Hygro-EF1A-EGFP-PARP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 23, 2021 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12683
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /note="RSV promoter"
                     /note="/application_notes=Strong promoter; drives 
                     transcription of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="5' LTR-Delta-U3"
                     /note="/application_notes=Allows transcription of viral RNA
                     and its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=-Psi
                     /label=nonstandard type: Miscellaneous
                     /note="Psi"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type: Miscellaneous
                     /note="RRE"
                     /note="/application_notes=Rev protein binding site that 
                     allows Rev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type: Miscellaneous
                     /note="cPPT"
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1959..3137
                     /label=EF1A
                     /note="EF1A"
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor 1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type: Miscellaneous
                     /note="Kozak"
                     /note="/application_notes=Facilitates translation 
                     initiation of ATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    3168..3884
                     /label=EGFP(ns)
                     /label=nonstandard type: ORF
                     /note="EGFP(ns)"
                     /note="/application_notes=Commonly used green fluorescent 
                     protein; ranked high in brightness, photostability and pH 
                     stability among all fluorescent proteins."
                     /note="/description=Enhanced green fluorescent protein; 
                     codon optimized based on a variant of wild type GFP from 
                     the jellyfish Aequorea victoria"
                     /note="/full_name=Enhanced green fluorescent protein(ns)"
                     /note="/official_designation=EGFP(ns)"
                     /note="/uuid=5443fd992fa248b69b274cf75346abad"
     misc_feature    3884..3885
                     /label=No linker
                     /label=nonstandard type: Linker
                     /note="No linker"
                     /note="/application_notes="
                     /note="/official_designation=No marker(ns)"
                     /note="/uuid=72e1edd2c1a540b28efbb477521f8696"
     CDS             3885..6929
                     /label=hPARP1[NM_001618.4]
                     /note="hPARP1[NM_001618.4]"
                     /note="/official_designation=NM_001618.3"
                     /note="/uuid=7ffeb1767f464014957e8b22b43298c6"
     misc_feature    6968..7565
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="WPRE"
                     /note="/application_notes=Enhances virus stability in 
                     packaging cells, leading to higher titer of packaged virus;
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     promoter        7584..8094
                     /label=mPGK promoter
                     /note="mPGK promoter"
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     misc_feature    8107..9132
                     /label=Hygro
                     /label=nonstandard type: ORF
                     /note="Hygro"
                     /note="/application_notes=Allows cells to be resistant to 
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             9203..9437
                     /label=3' LTR-Delta-U3
                     /note="3' LTR-Delta-U3"
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    9510..9644
                     /label=SV40 early pA
                     /note="SV40 early pA"
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     misc_feature    10598..11458
                     /label=Ampicillin
                     /label=nonstandard type: ORF
                     /note="Ampicillin"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampicillin."
                     /note="/description=Ampicillin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      11629..12217
                     /direction=RIGHT
                     /label=pUC ori
                     /note="pUC ori"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gtgagcaagg
     3181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3301 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3361 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3601 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3661 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3781 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3841 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagatggcg gagtcttcgg
     3901 ataagctcta tcgagtcgag tacgccaaga gcgggcgcgc ctcttgcaag aaatgcagcg
     3961 agagcatccc caaggactcg ctccggatgg ccatcatggt gcagtcgccc atgtttgatg
     4021 gaaaagtccc acactggtac cacttctcct gcttctggaa ggtgggccac tccatccggc
     4081 accctgacgt tgaggtggat gggttctctg agcttcggtg ggatgaccag cagaaagtca
     4141 agaagacagc ggaagctgga ggagtgacag gcaaaggcca ggatggaatt ggtagcaagg
     4201 cagagaagac tctgggtgac tttgcagcag agtatgccaa gtccaacaga agtacgtgca
     4261 aggggtgtat ggagaagata gaaaagggcc aggtgcgcct gtccaagaag atggtggacc
     4321 cggagaagcc acagctaggc atgattgacc gctggtacca tccaggctgc tttgtcaaga
     4381 acagggagga gctgggtttc cggcccgagt acagtgcgag tcagctcaag ggcttcagcc
     4441 tccttgctac agaggataaa gaagccctga agaagcagct cccaggagtc aagagtgaag
     4501 gaaagagaaa aggcgatgag gtggatggag tggatgaagt ggcgaagaag aaatctaaaa
     4561 aagaaaaaga caaggatagt aagcttgaaa aagccctaaa ggctcagaac gacctgatct
     4621 ggaacatcaa ggacgagcta aagaaagtgt gttcaactaa tgacctgaag gagctactca
     4681 tcttcaacaa gcagcaagtg ccttctgggg agtcggcgat cttggaccga gtagctgatg
     4741 gcatggtgtt cggtgccctc cttccctgcg aggaatgctc gggtcagctg gtcttcaaga
     4801 gcgatgccta ttactgcact ggggacgtca ctgcctggac caagtgtatg gtcaagacac
     4861 agacacccaa ccggaaggag tgggtaaccc caaaggaatt ccgagaaatc tcttacctca
     4921 agaaattgaa ggttaaaaaa caggaccgta tattcccccc agaaaccagc gcctccgtgg
     4981 cggccacgcc tccgccctcc acagcctcgg ctcctgctgc tgtgaactcc tctgcttcag
     5041 cagataagcc attatccaac atgaagatcc tgactctcgg gaagctgtcc cggaacaagg
     5101 atgaagtgaa ggccatgatt gagaaactcg gggggaagtt gacggggacg gccaacaagg
     5161 cttccctgtg catcagcacc aaaaaggagg tggaaaagat gaataagaag atggaggaag
     5221 taaaggaagc caacatccga gttgtgtctg aggacttcct ccaggacgtc tccgcctcca
     5281 ccaagagcct tcaggagttg ttcttagcgc acatcttgtc cccttggggg gcagaggtga
     5341 aggcagagcc tgttgaagtt gtggccccaa gagggaagtc aggggctgcg ctctccaaaa
     5401 aaagcaaggg ccaggtcaag gaggaaggta tcaacaaatc tgaaaagaga atgaaattaa
     5461 ctcttaaagg aggagcagct gtggatcctg attctggact ggaacactct gcgcatgtcc
     5521 tggagaaagg tgggaaggtc ttcagtgcca cccttggcct ggtggacatc gttaaaggaa
     5581 ccaactccta ctacaagctg cagcttctgg aggacgacaa ggaaaacagg tattggatat
     5641 tcaggtcctg gggccgtgtg ggtacggtga tcggtagcaa caaactggaa cagatgccgt
     5701 ccaaggagga tgccattgag cacttcatga aattatatga agaaaaaacc gggaacgctt
     5761 ggcactccaa aaatttcacg aagtatccca aaaagttcta ccccctggag attgactatg
     5821 gccaggatga agaggcagtg aagaagctga cagtaaatcc tggcaccaag tccaagctcc
     5881 ccaagccagt tcaggacctc atcaagatga tctttgatgt ggaaagtatg aagaaagcca
     5941 tggtggagta tgagatcgac cttcagaaga tgcccttggg gaagctgagc aaaaggcaga
     6001 tccaggccgc atactccatc ctcagtgagg tccagcaggc ggtgtctcag ggcagcagcg
     6061 actctcagat cctggatctc tcaaatcgct tttacaccct gatcccccac gactttggga
     6121 tgaagaagcc tccgctcctg aacaatgcag acagtgtgca ggccaaggtg gaaatgcttg
     6181 acaacctgct ggacatcgag gtggcctaca gtctgctcag gggagggtct gatgatagca
     6241 gcaaggatcc catcgatgtc aactatgaga agctcaaaac tgacattaag gtggttgaca
     6301 gagattctga agaagccgag atcatcagga agtatgttaa gaacactcat gcaaccacac
     6361 acaatgcgta tgacttggaa gtcatcgata tctttaagat agagcgtgaa ggcgaatgcc
     6421 agcgttacaa gccctttaag cagcttcata accgaagatt gctgtggcac gggtccagga
     6481 ccaccaactt tgctgggatc ctgtcccagg gtcttcggat agccccgcct gaagcgcccg
     6541 tgacaggcta catgtttggt aaagggatct atttcgctga catggtctcc aagagtgcca
     6601 actactgcca tacgtctcag ggagacccaa taggcttaat cctgttggga gaagttgccc
     6661 ttggaaacat gtatgaactg aagcacgctt cacatatcag caagttaccc aagggcaagc
     6721 acagtgtcaa aggtttgggc aaaactaccc ctgatccttc agctaacatt agtctggatg
     6781 gtgtagacgt tcctcttggg accgggattt catctggtgt gaatgacacc tctctactat
     6841 ataacgagta cattgtctat gatattgctc aggtaaatct gaagtatctg ctgaaactga
     6901 aattcaattt taagacctcc ctgtggtaaa cccagctttc ttgtacaaag tggtgataat
     6961 cgaattccga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7021 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7081 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7141 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7201 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7261 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7321 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7381 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7441 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7501 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7561 atcgggaatt cccgcggttc gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     7621 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     7681 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     7741 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     7801 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     7861 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     7921 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     7981 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     8041 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctcacgtg
     8101 gccaccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     8161 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     8221 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     8281 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     8341 gacattgggg aatttagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     8401 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     8461 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     8521 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     8581 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     8641 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     8701 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     8761 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     8821 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     8881 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     8941 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     9001 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     9061 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     9121 gcaaaggaat agggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     9181 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     9241 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9301 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9361 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9421 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     9481 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     9541 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9601 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     9661 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9721 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9781 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     9841 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     9901 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     9961 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
    10021 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    10081 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    10141 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    10201 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    10261 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    10321 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    10381 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    10441 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
    10501 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    10561 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    10621 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10681 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10741 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10801 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
    10861 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10921 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10981 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    11041 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    11101 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    11161 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    11221 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    11281 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    11341 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    11401 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    11461 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    11521 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    11581 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    11641 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    11701 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11761 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11821 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11881 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11941 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    12001 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaagaga gaaaggcgga
    12061 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    12121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    12181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    12241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    12301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    12361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    12421 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    12481 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    12541 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    12601 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
    12661 acaaaagctg gagctgcaag ctt
//
