LOCUS       pMC-X4                  4253 bp ds-DNA     circular     15-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    422..943
                     /label="X-4/UP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    995..1137
                     /label="ConS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     RBS             1297..1308
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1315..2028
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      2040..2111
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2127..2154
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2172..2314
                     /label="ConE"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2430..2880
                     /label="X-4/DW"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(2935..3750)
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      complement(join(3899..4253,1..409))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
ORIGIN
        1 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
       61 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      121 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      181 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      241 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      301 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      361 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgccaa
      421 tcccaaagct aagagtccca ttttattctt ctatatgtat attttcgata ctctaaacca
      481 ccctacaatg tagccctata ctaaatctgc tcaattttca gcttctacaa gtgactcgag
      541 gccacgtgga aagatccaac tactccagca caacgattca atataatcga ttgctccact
      601 cataagaggc aagaacaagc ttcaactttt ggtaagccgc cgtttataaa cagggaagat
      661 gtcctttgtc aagggaggca cagagcatgg ccaatttggc aaattgcagg tttttctgag
      721 tgaaaaatga aaaagcattg tagtagagtc ggctcactga aaaaccgggg aggacgaaaa
      781 ggtttccagc cacagttgta gtcacgtgcg cgccatgctg actaatggca gccgtcgttg
      841 ggcagaagag aattagtatg gtacaggata cgctaattgc gctccaacta ccaaggttgt
      901 tgagggaaca ctggggcaat aggctgtcgc cattcaagag cagattaagt cctcagcgag
      961 ctcgCCCTga attcgcatct agactgatga gacgtggtag agccacaaac agccggtaca
     1021 agcaacgatc tccaggacca tctgaatcat gcgcggatga cacgaactca cgacggcgat
     1081 cacagacatt aacccacagt acagacactg cgacaacgtg gcaattcgtc gcaatacaac
     1141 gtgagaccga aagtgaaacg tgatttcatg cgtcattttg aacattttgt aaatcttatt
     1201 taataatgtg tgcggcaatt cacatttaat ttatgaatgt tttcttaaca tcgcggcaac
     1261 tcaagaaacg gcaggttcgg atcttagcta ctagagaaag aggagaaata ctagatgcgt
     1321 aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc
     1381 aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg
     1441 acgctgaagt tcatctgtac tactggtaaa ctgccggttc cttggccgac tctggtaacg
     1501 acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac
     1561 ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat
     1621 gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc
     1681 attgagctga aaggcattga ctttaaagag gacggcaata tcctgggcca taagctggaa
     1741 tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa
     1801 gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac
     1861 cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc
     1921 acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag
     1981 ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaatg accaggcatc
     2041 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2101 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     2161 ggtctcagct ggaaatctgc tcgtcagtgg tgctcacact gacgaatcat gtacagatca
     2221 taccgatgac tgcctggcga ctcacaacta agcaagacag ccggaaccag cgccggcgaa
     2281 caccactgca tatatggcat atcacaacag tccacgtctc aagcagttac agagatgtta
     2341 cgaaccacta gtgcactgca gtacaactag agatctatgt gaggatccta actcgagatc
     2401 gaGAGTcctg caggactagt gctgaggcat taatacatgg gaagattcgc tttttttttt
     2461 tgaattacaa tagtatgtct gatgtctgca agaagtaaca ggcgtgtgca caagaatacg
     2521 tgtgtgtgcg taagcgtatg cactggtggc ataacttatc taagaagtat atatcactga
     2581 catagaaatg tagatataca ggtatttttc tcgataatcg ataaaaatct cgtcgcgctg
     2641 aaccaaactt ggtggttacg gagagttttt ctctcatcat tactgtcttt cgcattgatt
     2701 tcccctttga ccgataaaat cccttggatt cataagatta aacaaagagg tgatcaaaga
     2761 gaaccctgtg aaagtttatg tttataaccg ggcataaagt gaactagaca ctttcaagaa
     2821 gccaaccaaa gcatgagtaa cgaagcttac cagcatgatc ataccgtaaa tcctcaccag
     2881 attaagacct cagcgCCGAg cggccgcgtg ttacaaccaa ttaaccaatt ctgattagaa
     2941 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     3001 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     3061 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     3121 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     3181 cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt
     3241 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     3301 agcgaggcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     3361 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     3421 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     3481 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     3541 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     3601 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     3661 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctgga
     3721 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     3781 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     3841 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagtc
     3901 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3961 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4021 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4081 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4141 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4201 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctc
//