LOCUS       pMCL008_28bp            3647 bp ds-DNA     circular     26-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(117..880)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     rep_origin      193..781
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    685..708
                     /label="grna"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    830..851
                     /label="Fw_sequencing"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        896..1307
                     /label="pRPR1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1224..1242
                     /label="TetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1224..1242
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    1247..1266
                     /label="MCL024"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(1354..1359)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     RBS             1463..1474
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1481..2194
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1929..1934
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2206..2277
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2293..2320
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_RNA        2335..2410
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2411..2438
                     /label="24bp Csy4"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      2439..2876
                     /label="RPR1t"
                     /ApEinfo_revcolor=#c4df9b
                     /ApEinfo_fwdcolor=#c4df9b
     promoter        2568..2573
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2894..2928
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2916..2938)
                     /label="MCL023"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2919..2938)
                     /label="Rv_sequencing"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(join(2999..3647,1..11))
                     /label="CmR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
       61 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
      121 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
      181 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      241 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      301 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      361 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      421 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      481 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      541 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      601 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      661 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      721 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      781 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      841 gttctttcct gcgttatccc ctgattctgt ggataaccgt agtcggtctc caacgggggg
      901 atctgccaat tgaacataac atggtagtta catatactag taatatggtt cggcacacat
      961 taaaagtata aaaactatct gaattacgaa ttacatatat tggtcataaa aatcaatcaa
     1021 tcatcgtgtg ttttatatgt ctcttatcta agtataagaa tatccatagt taatattcac
     1081 ttacgctacc ttttaacctg taatcattgt caacaggata tgttaacgac ccacattgat
     1141 aaacgctagt atttcttttt cctcttctta ttggccggct gtctctatac tcccctatag
     1201 tctgtttctt ttcgtttcga ttgtccctat cagtgataga gatggcgcac atggtacgct
     1261 gtggtgctcg cggctgggaa cgaaactctg ggagctgcga ttggcagtga gacggaaagt
     1321 gaaacgtgat ttcatgcgtc attttgaaca ttttgtaaat cttatttaat aatgtgtgcg
     1381 gcaattcaca tttaatttat gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag
     1441 gttcggatct tagctactag agaaagagga gaaatactag atgcgtaaag gcgaagagct
     1501 gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg gtcataagtt
     1561 ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc tgaagttcat
     1621 ctgtactact ggtaaactgc cggttccttg gccgactctg gtaacgacgc tgacttatgg
     1681 tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct tcaagtccgc
     1741 catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg gcacgtacaa
     1801 aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg agctgaaagg
     1861 cattgacttt aaagaggacg gcaatatcct gggccataag ctggaataca attttaacag
     1921 ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga attttaaaat
     1981 tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc aaaacactcc
     2041 aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc aaagcgttct
     2101 gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg taaccgcagc
     2161 gggcatcacg catggtatgg atgaactgta caaatgacca ggcatcaaat aaaacgaaag
     2221 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     2281 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatacgtc tcaggtttta
     2341 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
     2401 gagtcggtgc gttcactgcc gtataggcag ctaagaaacc atatccaact tccaatttaa
     2461 tctttctttt ttaattttca cttatttgcg atacagaaag aaaaaagcga tagtaactat
     2521 tgaattttgt ttggatttgg ttagattaga tatggtttct ctttatattt acatgctaaa
     2581 aatgggctac accagagata cataattaga tatatatacg ccagtacacc ttatcggccc
     2641 aagccttgtc ccaaggcagc gttttgttct tggaaacgct gccctacacg ttcgctatgc
     2701 ttcaagaact tttctgagca cttcatgatg catgtttgtt ccttattggt tagctttgat
     2761 gttgtgaagt cattgacaca gtctgtgaaa catctttcta ccagattaga gtacaaacgc
     2821 atgaaatcct tcatttgctt ttgttccact actttttgga actcttgttg ttctttgctg
     2881 tgagaccaga ccaataaaaa acgcccggcg gcaaccgaGC GTTCTGAACA AATCCAGAtg
     2941 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     3001 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     3061 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     3121 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     3181 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct
     3241 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     3301 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     3361 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     3421 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     3481 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     3541 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     3601 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgatt
//