LOCUS       pMCL009                 5589 bp ds-DNA     circular     10-APR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(77..207)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(208..971)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    789..811
                     /label="snr52"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    complement(980..983)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    984..1252
                     /label="pSNR52 misc_feature"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1134..1158
                     /label="pmcl9_seq_fw"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    complement(1254..1259)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1260..1389
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     RBS             1390..1425
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1426..2142
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      2143..2271
                     /label="BBa_B0015 Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    2272..2277
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2279..2357
                     /label="spacer RNA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2321..2346)
                     /label="pmcl9_seq_rv"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2358..2377
                     /label="tSUP4 misc_feature"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(2377..2403)
                     /label="pmcl19_seq_rv_correct"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2383..2630
                     /label="tCYC1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    2631..2634
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        2635..2811
                     /label="ScURA3 Promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2694..2714)
                     /label="term"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2812..3615
                     /label="URA3"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3534..3556
                     /label="2mic"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      3616..3693
                     /label="ScURA3 Terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    complement(3694..3697)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      3698..4660
                     /label="2micron"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      4661..4698
                     /label="FRT site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    complement(4699..4702)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      complement(4711..4807)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(join(4808..5589,1..76))
                     /label="AmpR"
                     /ApEinfo_revcolor=#9f4240
                     /ApEinfo_fwdcolor=#9f4240
ORIGIN
        1 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
       61 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
      121 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      181 gcacatttcc ccgaaaagtg ccacctgtca tgaccaaaat cccttaacgt gagttttcgt
      241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      901 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      961 tggataaccg tgcggccgcc ccttctttga aaagataatg tatgattatg ctttcactca
     1021 tatttataca gaaacttgat gttttctttc gagtatatac aaggtgatta catgtacgtt
     1081 tgaagtacaa ctctagattt tgtagtgccc tcttgggcta gcggtaaagg tgcgcatttt
     1141 ttcacaccct acaatgttct gttcaaaaga ttttggtcaa acgctgtaga agtgaaagtt
     1201 ggtgcgcatg tttcggcgtt cgaaacttct ccgcagtgaa agataaatga tctgagacgg
     1261 aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt
     1321 gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac
     1381 ggcaggttcg gatcttagct actagagaaa gaggagaaat actagatgcg taaaggcgaa
     1441 gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat
     1501 aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag
     1561 ttcatctgta ctactggtaa actgccggtt ccttggccga ctctggtaac gacgctgact
     1621 tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag
     1681 tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg
     1741 tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg
     1801 aaaggcattg actttaaaga ggacggcaat atcctgggcc ataagctgga atacaatttt
     1861 aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt
     1921 aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac
     1981 actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc
     2041 gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc
     2101 gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat gaccaggcat caaataaaac
     2161 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2221 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat acgtctcagt
     2281 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     2341 caccgagtcg gtggtgcttt ttttgttttt tatgtcttcg agtcatgtaa ttagttatgt
     2401 cacgcttacg ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac
     2461 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat
     2521 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac
     2581 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc tacacggttt
     2641 ccttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac
     2701 agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta
     2761 ttcttaaccc aactgcacag aacaaaaacg tgcaggaaac gaagataaat catgtcgaaa
     2821 gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat
     2881 atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa
     2941 ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat
     3001 atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag
     3061 tacaattttt tactcttcga ggacagaaaa tttgctgaca ttggtaatac agtcaaattg
     3121 cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt
     3181 gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa
     3241 cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa
     3301 tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt
     3361 gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt
     3421 gtgggtttag atgacaaggg agatgcattg ggtcaacagt atagaaccgt ggatgatgtg
     3481 gtttctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat
     3541 gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc
     3601 ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt
     3661 agagcttcaa tttaattata tcagttatta cccgagtaac gaagcatctg tgcttcattt
     3721 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     3781 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
     3841 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     3901 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
     3961 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
     4021 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
     4081 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
     4141 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
     4201 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
     4261 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
     4321 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
     4381 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
     4441 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
     4501 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
     4561 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
     4621 agtgcgtcat cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
     4681 ctagagaata ggaacttccc gagcggccgc gattatcaaa aaggatcttc acctagatcc
     4741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4921 gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa
     4981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5101 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5401 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5581 ccagcgttt
//